{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:31:11Z","timestamp":1760243471685,"version":"build-2065373602"},"reference-count":26,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2013,3,14]],"date-time":"2013-03-14T00:00:00Z","timestamp":1363219200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The study aimed to clarify the role of electric pulses in combination with chemotherapy on the viability of keratinocyte cell line HaCaT, in the context of its application as a new therapeutic approach for psoriasis. The data show that electroporation of HaCaT cells in combination with rifampicin induces cytoskeleton disruption and increases permeability of cell monolayer due to cell-cell junctions\u2019 interruption, visualized by fluorescent imaging of E-cadherin and actin integrity. This was accompanied with synergistic reduction of cell viability. The study proposes a new opportunity for more effective skin treatment than chemotherapy. The future application of this electrochemotherapeutic approach for combined local treatment of psoriasis may have serous benefits because of a high possibility to avoid side-effects of conventional chemotherapy.<\/jats:p>","DOI":"10.3390\/s130303625","type":"journal-article","created":{"date-parts":[[2013,3,14]],"date-time":"2013-03-14T12:22:04Z","timestamp":1363263724000},"page":"3625-3634","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Fluorescent Imaging for Assessment of the Effect of Combined Application of Electroporation and Rifampicin on HaCaT Cells as a New Therapeutic Approach for Psoriasis"],"prefix":"10.3390","volume":"13","author":[{"given":"Biliana","family":"Nikolova","sequence":"first","affiliation":[{"name":"Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences,  Acad.G. Bonchev Str., bl. 21, Sofia 1113, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anelia","family":"Kostadinova","sequence":"additional","affiliation":[{"name":"Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences,  Acad.G. Bonchev Str., bl. 21, Sofia 1113, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Borislav","family":"Dimitrov","sequence":"additional","affiliation":[{"name":"Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences,  Acad.G. Bonchev Str., bl. 21, Sofia 1113, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhivko","family":"Zhelev","sequence":"additional","affiliation":[{"name":"Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences,  Acad.G. Bonchev Str., bl. 21, Sofia 1113, Bulgaria"},{"name":"Medical Faculty, Trakia University, 11 Armeiska Str., Stara Zagora 6000, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rumiana","family":"Bakalova","sequence":"additional","affiliation":[{"name":"Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan"},{"name":"Medical Faculty, Sofia University, 1 Koziak Str., Sofia 1407, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ichio","family":"Aoki","sequence":"additional","affiliation":[{"name":"Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsuneo","family":"Saga","sequence":"additional","affiliation":[{"name":"Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iana","family":"Tsoneva","sequence":"additional","affiliation":[{"name":"Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences,  Acad.G. Bonchev Str., bl. 21, Sofia 1113, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2013,3,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1016\/S0006-3495(96)79288-3","article-title":"Calcium mediated DNA adsorption to yeast cells and kinetiks of cell transformation by electroporation","volume":"71","author":"Neumann","year":"1996","journal-title":"Biophys. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2336","DOI":"10.1038\/bjc.1998.388","article-title":"Effective treatment of cutaneous and subcutaneous malignant tumors by electrochemotherapy","volume":"77","author":"Mir","year":"1998","journal-title":"Br. J. Cancer"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2522","DOI":"10.5504\/BBEQ.2011.0059","article-title":"Treatment of Melanoma by electroporation of bacillus Calmette-Guerin","volume":"25","author":"Nikolova","year":"2011","journal-title":"Biotechnol. Biotechnol. Eq."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Tsoneva, I., Iordanov, I., Berger, A., Tomov, T., Nikolova, B., Mudrov, N., and Berger, M.R. (2010). Electrodelivery of drugs into cancer cells in the presence of poloxamer 188. J. Biomed. Biotechnol.","DOI":"10.1155\/2010\/314213"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.bioelechem.2006.10.006","article-title":"Electrochemotherapy of Mycosis fungoides by interferon-alpha","volume":"70","author":"Peycheva","year":"2007","journal-title":"Bioelectrochemistry"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Dotsinsky, I., Nikolova, B., Peycheva, E., and Tsoneva, I. (2012). New modality for electrochemotherapy of surface tumors. Biotechnol. Biotechnolog. Eq., 26.","DOI":"10.5504\/BBEQ.2012.0098"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1111\/j.1939-1676.2011.0678.x","article-title":"Evaluation of Cisplatin as an electrochemotherapy agent for the treatment of incompletely excised mast cell tumors in dogs","volume":"25","author":"Spugnini","year":"2011","journal-title":"J. Vet. Intern. Med."},{"key":"ref_8","first-page":"372","article-title":"Rifampicin-from tuberculosis to psoriasis?","volume":"30","author":"Nissen","year":"2011","journal-title":"Aus. Pharm."},{"key":"ref_9","first-page":"21","article-title":"Eruptive psoriasis and treatment of rifampicin","volume":"3","author":"Tsankov","year":"2005","journal-title":"Trakia J. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1080\/09546630500375643","article-title":"Old drug-new indication. Rifampicin in psoriasis","volume":"17","author":"Tsankov","year":"2006","journal-title":"J. Dermatol. Treat."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1111\/j.1468-3083.2008.02721.x","article-title":"Rifampicin in the treatment of psoriasis","volume":"23","author":"Tsankov","year":"2009","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1128\/AAC.00691-09","article-title":"Pretreatment of epithelian cells with rifaximin alters bacterial attachment and internalization profiles","volume":"54","author":"Brown","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.taap.2009.06.022","article-title":"Altered integrity and decreased expression of hepatocyte tight junctions in rifampicin-induced cholestasis in mice","volume":"240","author":"Chen","year":"2009","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1007\/s12013-011-9244-y","article-title":"Time-dependent micromechanical responses of breast cancer cells and adjacent fibroblasts to electric treatment","volume":"61","author":"Yizraeli","year":"2011","journal-title":"Cell. Biochem. Biophys."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3145","DOI":"10.1158\/1535-7163.MCT-06-0410","article-title":"The endothelial cytoskeleton as a target of electroporatio-based therapies","volume":"5","author":"Kanthou","year":"2006","journal-title":"Mol. Cancer Ther."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Pehlivanova, V., Tsoneva, I., and Tzoneva, R. (2012). Multiple effects of electroporation on the adhesive behavior of breast cancer cells and fibroblst. Cancer Cell. Int., 12.","DOI":"10.1186\/1475-2867-12-9"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1038\/mt.2010.303","article-title":"The actin cytoskeleton has an active role in the electrotransfer of plasmid DNA in mammalian cells","volume":"19","author":"Rosazza","year":"2011","journal-title":"Mol. Ther."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1016\/j.bpj.2008.09.035","article-title":"Regulation of cell cytoskeleton and membrane mechanics by electric field: Role of linker proteins","volume":"96","author":"Titushkin","year":"2009","journal-title":"Biophys. J."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0014-4835(02)00257-9","article-title":"Bi-directional migration of lens epithelial cells in a physiological electrical field","volume":"76","author":"Wang","year":"2003","journal-title":"Exp. Eye Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1111\/j.1748-5827.2010.00913.x","article-title":"Bilateral pleomorphic rhabdomyosarcoma in a cat treated with electrochemotherapy","volume":"51","author":"Spugnini","year":"2010","journal-title":"J. Small Anim. Pract."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/ijc.2910200102","article-title":"Binding of soluble form of fibroblast surface protein, fibronectin, to collagen","volume":"20","author":"Engvall","year":"1977","journal-title":"Int. J. Cancer"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/0092-8674(92)90115-S","article-title":"Integrins: Versatility modulation and signaling in cell adhesion","volume":"69","author":"Hynes","year":"1992","journal-title":"Cell"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e52713","DOI":"10.1371\/journal.pone.0052713","article-title":"Differential cellular effects of electroporation and electrochemotherapy in monolyers of human microvascular endothelial cells","volume":"7","author":"Meulenberg","year":"2012","journal-title":"PLoS One"},{"key":"ref_25","first-page":"83","article-title":"Bacterial interference with host epithelial junctional complexes: Probiotic bacteria vs. A\/E lesion-forming Escherichia coli","volume":"1","author":"Stephanova","year":"2012","journal-title":"J. BioSci. Biotech."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1042\/CBI20100464","article-title":"Effect of actin cytoskeleton disruption on electric pulse-induced apoptosis and electroporation in tumour cells","volume":"35","author":"Xiao","year":"2011","journal-title":"Cell. Biol. Int."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/13\/3\/3625\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:45:32Z","timestamp":1760219132000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/13\/3\/3625"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3,14]]},"references-count":26,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2013,3]]}},"alternative-id":["s130303625"],"URL":"https:\/\/doi.org\/10.3390\/s130303625","relation":{},"ISSN":["1424-8220"],"issn-type":[{"type":"electronic","value":"1424-8220"}],"subject":[],"published":{"date-parts":[[2013,3,14]]}}}