{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T04:08:11Z","timestamp":1768450091833,"version":"3.49.0"},"reference-count":17,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2013,4,18]],"date-time":"2013-04-18T00:00:00Z","timestamp":1366243200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>This study was conducted to develop a simple, rapid, and accurate lateral flow immunoassay (LFIA) detection method for point-of-care diagnosis. The one-dot LFIA for aflatoxin B1 (AFB1) was based on the modified competitive binding format using competition between AFB1 and colloidal gold-AFB1-BSA conjugate for antibody binding sites in the test zone. A Smartphone-based reading system consisting of a Samsung Galaxy S2 Smartphone, a LFIA reader, and a Smartphone application for the image acquisition and data analysis. The detection limit of one-dot LFIA for AFB1 is 5 \u03bcg\/kg. This method provided semi-quantitative analysis of AFB1 samples in the range of 5 to 1,000 \u03bcg\/kg. Using combination of the one-dot LFIA and the Smartphone-based reading system, it is possible to conduct a more fast and accurate point-of-care diagnosis.<\/jats:p>","DOI":"10.3390\/s130405109","type":"journal-article","created":{"date-parts":[[2013,4,18]],"date-time":"2013-04-18T11:45:04Z","timestamp":1366285504000},"page":"5109-5116","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":104,"title":["Performance Improvement of the One-Dot Lateral Flow Immunoassay for Aflatoxin B1 by Using a Smartphone-Based Reading System"],"prefix":"10.3390","volume":"13","author":[{"given":"Sangdae","family":"Lee","sequence":"first","affiliation":[{"name":"Post-Harvest & Food Engineering Division, Department of Agricultural Engineering,  National Academy of Agricultural Sciences, Suwon 441707, Korea"}]},{"given":"Giyoung","family":"Kim","sequence":"additional","affiliation":[{"name":"Post-Harvest & Food Engineering Division, Department of Agricultural Engineering,  National Academy of Agricultural Sciences, Suwon 441707, Korea"}]},{"given":"Jihea","family":"Moon","sequence":"additional","affiliation":[{"name":"Post-Harvest & Food Engineering Division, Department of Agricultural Engineering,  National Academy of Agricultural Sciences, Suwon 441707, Korea"}]}],"member":"1968","published-online":{"date-parts":[[2013,4,18]]},"reference":[{"key":"ref_1","unstructured":"Galvano, F., Ritieni, A., Piva, G., and Pietri, A. 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Actuators B"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"11684","DOI":"10.3390\/s120911684","article-title":"A fast and sensitive quantitative lateral flow immunoassay for Cry1Ab based on a novel signal amplification conjugate","volume":"12","author":"Chen","year":"2012","journal-title":"Sensors"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"634","DOI":"10.3390\/ma5040634","article-title":"A gold nanoparticle and aflatoxin B1-BSA conjugates based lateral flow assay method for the analysis of aflatoxin B1","volume":"5","author":"Moon","year":"2012","journal-title":"Materials"}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/13\/4\/5109\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:46:13Z","timestamp":1760219173000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/13\/4\/5109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4,18]]},"references-count":17,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2013,4]]}},"alternative-id":["s130405109"],"URL":"https:\/\/doi.org\/10.3390\/s130405109","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4,18]]}}}