{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T12:16:27Z","timestamp":1772021787540,"version":"3.50.1"},"reference-count":25,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2013,6,17]],"date-time":"2013-06-17T00:00:00Z","timestamp":1371427200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A novel and integrated membrane sensing platform for DNA detection is developed based on an anodic aluminum oxide (AAO) membrane. Platinum electrodes (~50\u2013100 nm thick) are coated directly on both sides of the alumina membrane to eliminate the solution resistance outside the nanopores. The electrochemical impedance technique is employed to monitor the impedance changes within the nanopores upon DNA binding. Pore resistance (Rp) linearly increases in response towards the increasing concentration of the target DNA in the range of 1 \u00d7 10\u221212 to 1 \u00d7 10\u22126 M. Moreover, the biosensor selectively differentiates the complementary sequence from single base mismatched (MM-1) strands and non-complementary strands. This study reveals a simple, selective and sensitive method to fabricate a label-free DNA biosensor.<\/jats:p>","DOI":"10.3390\/s130607774","type":"journal-article","created":{"date-parts":[[2013,6,17]],"date-time":"2013-06-17T11:18:49Z","timestamp":1371467929000},"page":"7774-7785","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":74,"title":["Impedimetric DNA Biosensor Based on a Nanoporous Alumina Membrane for the Detection of the Specific Oligonucleotide Sequence of Dengue Virus"],"prefix":"10.3390","volume":"13","author":[{"given":"Jiajia","family":"Deng","sequence":"first","affiliation":[{"name":"Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chee-Seng","family":"Toh","sequence":"additional","affiliation":[{"name":"Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2013,6,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Bian, G., Xu, Y., Lu, P., Xie, Y., and Xi, Z. (2010). The endosymbiotic bacterium Wolbachia induces resistance to dengue virus in Aedes aegypti. PLoS Pathog., 6.","DOI":"10.1371\/journal.ppat.1000833"},{"key":"ref_2","first-page":"44","article-title":"Dengue in the Americas","volume":"26","author":"Wilson","year":"2002","journal-title":"Dengue Bull."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"693","DOI":"10.4269\/ajtmh.1999.60.693","article-title":"Rapid serologic diagnosis of dengue virus infection using a commercial capyure ELISA that distinguishes primary and secondary infections","volume":"60","author":"Vaughn","year":"1999","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jviromet.2009.10.001","article-title":"A real-time PCR procedure for detection of dengue virus serotypes 1, 2, and 3, and their quantitation in clinical and laboratory samples","volume":"163","author":"Conceicao","year":"2010","journal-title":"J. Virol. Methods"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"7556","DOI":"10.1021\/nn301368z","article-title":"Nanochannels preparation and application in biosensing","volume":"6","year":"2012","journal-title":"ACS Nano"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.trac.2012.11.007","article-title":"Nanoporous anodic aluminum oxide for chemical sensing and biosensors","volume":"44","author":"Santos","year":"2013","journal-title":"Trends Anal. Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/j.talanta.2008.12.001","article-title":"A novel electrochemical biosensor based on dynamic polymerase-extending hybridization for E. coli O157:H7 DNA detection","volume":"78","author":"Wang","year":"2009","journal-title":"Talanta"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1039\/b608084g","article-title":"Hydrothermally shrunk alumina nanopores and their application to DNA sensing","volume":"131","author":"Takmakov","year":"2006","journal-title":"Analyst"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"6417","DOI":"10.1021\/nn101050r","article-title":"A nanochannel array-based electrochemical device for quantitative label-free DNA analysis","volume":"4","author":"Li","year":"2010","journal-title":"ACS Nano"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.bioelechem.2012.04.006","article-title":"Electrochemical impedance spectroscopy characterization of nanoporous alumina dengue virus biosensor","volume":"88","author":"Nguyen","year":"2012","journal-title":"Bioelectrochemistry"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Rai, V., Hapuarachchi, H.C., Ng, L.C., Soh, S.H., Leo, Y.S., and Toh, C.-S. (2012). Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor. PLoS One, 7.","DOI":"10.1371\/journal.pone.0042346"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.talanta.2012.06.055","article-title":"Electrochemical nanoporous alumina membrane-based label-free DNA biosensor for the detection of Legionella sp","volume":"98","author":"Rai","year":"2012","journal-title":"Talanta"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5616","DOI":"10.3390\/s110605616","article-title":"Label-free electrochemical detection of the specific oligonucleotide sequence of dengue virus type 1 on pencil graphite electrodes","volume":"11","author":"Souza","year":"2011","journal-title":"Sensors"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S0003-2670(02)00670-0","article-title":"Electrochemical detection of DNA hybridization based on silver-enhanced gold nanoparticle label","volume":"469","author":"Cai","year":"2002","journal-title":"Anal. Chim. Acta"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"9007","DOI":"10.1039\/c0cc02683b","article-title":"Nanoparticle based enhancement of electrochemical DNA hybridization signal using nanoporous electrodes","volume":"46","year":"2010","journal-title":"Chem. Comm."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.ab.2006.01.018","article-title":"Optical detection of DNA hybridization based on fluorescence quenching of tagged oligonucleotide probes by gold nanoparticles","volume":"353","author":"Wu","year":"2006","journal-title":"Anal. Biochem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2579","DOI":"10.1016\/j.bios.2010.04.026","article-title":"Localized surface plasmon-enhanced fluorescence spectroscopy for highly-sensitive real-time detection of DNA hybridization","volume":"25","author":"Touahir","year":"2010","journal-title":"Biosens. Bioelectron."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1543","DOI":"10.1016\/j.bios.2010.07.108","article-title":"DNA microarrays for hybridization detection by surface Plasmon resonance spectroscopy","volume":"26","author":"Kick","year":"2010","journal-title":"Biosens. Bioelectron."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.snb.2006.08.012","article-title":"Enhanced surface Plasmon resonance for detection of DNA hybridization based on layer-by-layer assembly films","volume":"123","author":"Wang","year":"2007","journal-title":"Sens.Actuators B"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/j.bios.2005.01.006","article-title":"Comparison of surface Plasmon resonance spectroscopy and quartz crystal microbalance techniques for studying DNA assembly and hybridization","volume":"21","author":"Su","year":"2005","journal-title":"Biosens. 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