{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T17:12:40Z","timestamp":1770484360239,"version":"3.49.0"},"reference-count":32,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2013,6,27]],"date-time":"2013-06-27T00:00:00Z","timestamp":1372291200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A monoclonal antibody specifically recognizing dibutyl phthalate (DBP) was prepared based on a hapten (di-n-butyl-4-aminophthalate). After optimizing various parameters such as concentrations of antibody, coating antigen and composition of the assay buffer, an inhibition curve was plotted with the 50% inhibition concentration value (IC50) 33.6 \u00b1 2.5 ng\/mL. A low level of cross-reactivity (&lt;5%) was found for other phthalate esters. Recovery tests were conducted using liquor simulant (a mixture of water and ethanol) at two fortification levels (100 ng\/mL and 300 ng\/mL). The recovery rates ranged from 84.7% to 94.5% with a coefficient of variation between 7.1% and 12.8%. Nine liquor samples of different alcoholic strengths were detected using the proposed measure and confirmatory analysis was performed using liquid chromatography-mass spectroscopy (LC-MS). The detection results showed good consistency between the two measures and all the data above indicated that the proposed ELISA could be applied in DBP screening.<\/jats:p>","DOI":"10.3390\/s130708331","type":"journal-article","created":{"date-parts":[[2013,6,28]],"date-time":"2013-06-28T03:27:52Z","timestamp":1372390072000},"page":"8331-8339","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Development of an Enzyme-Linked Immunosorbent Assay for Dibutyl Phthalate in Liquor"],"prefix":"10.3390","volume":"13","author":[{"given":"Hua","family":"Kuang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liqiang","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liguang","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingling","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Libing","family":"Wang","sequence":"additional","affiliation":[{"name":"Research Centre of Hunan Entry\u2013Exit Inspection and Quarantine Bureau, Changsha 410001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuanlai","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2013,6,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6789","DOI":"10.1007\/s10661-011-2458-z","article-title":"Distributions of phthalic esters carried by total suspended particulates in Nanjing, China","volume":"184","author":"Wang","year":"2012","journal-title":"Environ. 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