{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T21:50:26Z","timestamp":1761429026152,"version":"build-2065373602"},"reference-count":44,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2013,9,3]],"date-time":"2013-09-03T00:00:00Z","timestamp":1378166400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>This paper concerns the development of an electrochemical biosensor for the determination of A\u03b216\u201323' and A\u03b21\u201340 peptides. The His-tagged V and VC1 domains of Receptor for Advanced Glycation end Products (RAGE) immobilized on a gold electrode surface were used as analytically active molecules. The immobilization of His6\u2013RAGE domains consists of: (i) formation of a mixed layer of N-acetylcysteamine (NAC) and the thiol derivative of pentetic acid (DPTA); (ii) complexation of Cu(II) by DPTA;  (iii) oriented immobilization of His6\u2013RAGE domains via coordination bonds between Cu(II) sites from DPTA\u2013Cu(II) complex and imidazole nitrogen atoms of a histidine  tag. Each modification step was controlled by cyclic voltammetry (CV), Osteryoung  square-wave voltammetry (OSWV), and atomic force microscopy (AFM). The applicability of the proposed biosensor was tested in the presence of human plasma, which had no influence on its performance. The detection limits for A\u03b21\u201340 determination were 1.06 nM and 0.80 nM, in the presence of buffer and human plasma, respectively. These values reach the concentration level of A\u03b21\u201340 which is relevant for determination of its soluble form in human plasma, as well as in brain. This indicates the promising future application of biosensor presented for early diagnosis of neurodegenerative diseases.<\/jats:p>","DOI":"10.3390\/s130911586","type":"journal-article","created":{"date-parts":[[2013,9,3]],"date-time":"2013-09-03T12:43:48Z","timestamp":1378212228000},"page":"11586-11602","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Oriented Immobilization of His-Tagged Protein on a Redox Active Thiol Derivative of DPTA-Cu(II) Layer Deposited on a Gold Electrode\u2014The Base of Electrochemical Biosensors"],"prefix":"10.3390","volume":"13","author":[{"given":"Edyta","family":"Miku\u0142a","sequence":"first","affiliation":[{"name":"Institute of Animal Reproduction and Food Research of Polish Academy of Sciences,  Tuwima 10, Olsztyn 10-748, Poland"}]},{"given":"Magdalena","family":"Sulima","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Biophysics of Polish Academy of Sciences, Pawi\u0144skiego 5a,  Warsaw 02-106, Poland"}]},{"given":"Ilona","family":"Marsza\u0142ek","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Biophysics of Polish Academy of Sciences, Pawi\u0144skiego 5a,  Warsaw 02-106, Poland"}]},{"given":"Aleksandra","family":"Wys\u0142ouch-Cieszy\u0144ska","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Biophysics of Polish Academy of Sciences, Pawi\u0144skiego 5a,  Warsaw 02-106, Poland"}]},{"given":"Peter","family":"Verwilst","sequence":"additional","affiliation":[{"name":"Chemistry Department, University of Leuven, Celestijnenlaan 200F, Leuven B-3001, Belgium"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9597-0629","authenticated-orcid":false,"given":"Wim","family":"Dehaen","sequence":"additional","affiliation":[{"name":"Chemistry Department, University of Leuven, Celestijnenlaan 200F, Leuven B-3001, Belgium"}]},{"given":"Jerzy","family":"Radecki","sequence":"additional","affiliation":[{"name":"Institute of Animal Reproduction and Food Research of Polish Academy of Sciences,  Tuwima 10, Olsztyn 10-748, Poland"}]},{"given":"Hanna","family":"Radecka","sequence":"additional","affiliation":[{"name":"Institute of Animal Reproduction and Food Research of Polish Academy of Sciences,  Tuwima 10, Olsztyn 10-748, Poland"}]}],"member":"1968","published-online":{"date-parts":[[2013,9,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1016\/j.bbapap.2011.02.011","article-title":"Binding studies of truncated variants of the A\u03b2 peptide to the V-domain of the RAGE receptor reveal A\u03b2 residues responsible for binding","volume":"1814","author":"Gospodarska","year":"2011","journal-title":"Biochim. 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