{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T09:34:02Z","timestamp":1776504842635,"version":"3.51.2"},"reference-count":27,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2013,9,25]],"date-time":"2013-09-25T00:00:00Z","timestamp":1380067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this study a novel sensitive nanogold particle sensor enhancement based  on mixed self-assembled monolayers was explored and used to construct a Surface  Plasmon Resonance (SPR) immunosensor to detect Ischemia Modified Albumin (IMA). Compared with a direct binding SPR assay at a limit of detection (LOD) of 100 ng\/L, gold nanoparticles (AuNPs) of 10 nm dramatically improved the LOD of IMA to 10 ng\/L. Meanwhile, no interfering substance that may lead to false positive results was identified. These results suggested that the SPR biosensor presented superior properties, and provided a simple label-free strategy to increase assay sensitivity for further acute coronary syndrome (ACS) diagnosis.<\/jats:p>","DOI":"10.3390\/s131012794","type":"journal-article","created":{"date-parts":[[2013,9,25]],"date-time":"2013-09-25T12:47:40Z","timestamp":1380113260000},"page":"12794-12803","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["A Gold Nanoparticles Enhanced Surface Plasmon Resonance Immunosensor for Highly Sensitive Detection of Ischemia-Modified Albumin"],"prefix":"10.3390","volume":"13","author":[{"given":"Guang","family":"Li","sequence":"first","affiliation":[{"name":"Laboratory of Biomedical Engineering, Chongqing Medical University, 1 Yixueyuan Road,  Chongqing 400016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xian","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, 174 Shapingba Main Street, Chongqing 400044, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng","family":"Yang","sequence":"additional","affiliation":[{"name":"Laboratory of Biomedical Engineering, Chongqing Medical University, 1 Yixueyuan Road,  Chongqing 400016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng-Meng","family":"Chen","sequence":"additional","affiliation":[{"name":"Laboratory of Biomedical Engineering, Chongqing Medical University, 1 Yixueyuan Road,  Chongqing 400016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Long-Cong","family":"Chen","sequence":"additional","affiliation":[{"name":"Laboratory of Biomedical Engineering, Chongqing Medical University, 1 Yixueyuan Road,  Chongqing 400016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing-Liang","family":"Xiong","sequence":"additional","affiliation":[{"name":"Laboratory of Biomedical Engineering, Chongqing Medical University, 1 Yixueyuan Road,  Chongqing 400016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2013,9,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.clinbiochem.2009.01.019","article-title":"Cardiac biomarkers and the case for point-of-care testing","volume":"42","author":"McDonnell","year":"2009","journal-title":"Clin. Biochem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.trsl.2011.11.002","article-title":"Biomarkers in acute myocardial injury","volume":"159","author":"Kehl","year":"2012","journal-title":"Transl. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1016\/j.cca.2011.04.003","article-title":"Biochemical markers in acute coronary syndrome","volume":"412","author":"Ramasamy","year":"2011","journal-title":"Clin. Chim. Acta"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.cca.2007.05.003","article-title":"Application of albumin-adjusted ischemia modified albumin index as an early screening marker for acute coronary syndrome","volume":"384","author":"Lee","year":"2007","journal-title":"Clin. Chim. 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Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1159\/000093000","article-title":"Ischemia-modified albumin concentrations should be interpreted with caution in patients with low serum albumin concentrations","volume":"15","author":"Gaze","year":"2006","journal-title":"Med. Princ. Pract."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.3390\/s110201565","article-title":"Overview of the characteristics of micro- and nano-structured surface plasmon resonance sensors","volume":"11","author":"Roh","year":"2011","journal-title":"Sensors"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.bios.2011.10.023","article-title":"Recent trends in antibody based sensors","volume":"34","author":"Holford","year":"2012","journal-title":"Biosens. Bioelectron."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1007\/s00216-006-0800-z","article-title":"Multi-analyte spr immunoassays for environmental biosensing of pesticides","volume":"387","author":"Mauriz","year":"2007","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0003-2670(02)00033-8","article-title":"Effect of hydrogel matrix on binding kinetics of protein-protein interactions on sensor surface","volume":"456","author":"Fong","year":"2002","journal-title":"Anal. Chim. Acta"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1016\/j.bios.2010.08.011","article-title":"A dual surface plasmon resonance assay for the determination of ribonuclease h activity","volume":"26","author":"Sipova","year":"2010","journal-title":"Biosens. Bioelectron."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1016\/j.foodchem.2011.10.109","article-title":"Recent developments and applications of surface plasmon resonance biosensors for the detection of mycotoxins in foodstuffs","volume":"132","author":"Li","year":"2012","journal-title":"Food Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.3390\/s9031339","article-title":"Toxin detection by surface plasmon resonance","volume":"9","author":"Hodnik","year":"2009","journal-title":"Sensors"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.ab.2005.05.001","article-title":"Sensitivity enhancement of surface plasmon resonance biosensing of small molecules","volume":"343","author":"Mitchell","year":"2005","journal-title":"Anal. Biochem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ab.2011.05.027","article-title":"Gold nanoparticle-based signal amplification for biosensing","volume":"417","author":"Cao","year":"2011","journal-title":"Anal. Biochem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.bios.2013.07.002","article-title":"Graphene oxide and dextran capped gold nanoparticles based surface plasmon resonance sensor for sensitive detection of concanavalin a","volume":"50","author":"Huang","year":"2013","journal-title":"Biosens. Bioelectron."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Azzam, E., Abd El-aal, A., Shekhah, O., Arslan, H., and W\u00f6ll, C. (2013). Fabrication of spr nanosensor using gold nanoparticles and self-assembled monolayer technique for detection of cu2+ in an aqueous solution. J. Dispers. Sci. Technol.","DOI":"10.1080\/01932691.2013.807741"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.bios.2007.03.025","article-title":"Sensitivity enhancement of spr assay of progesterone based on mixed self-assembled monolayers using nanogold particles","volume":"23","author":"Yuan","year":"2007","journal-title":"Biosens. Bioelectron."},{"key":"ref_20","first-page":"1637","article-title":"Anodic stripping voltammetric immunoassay based on silver enhanced gold label for determination of complement iii","volume":"26","author":"Chu","year":"2005","journal-title":"Chem. J. Chin. Univ."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2740","DOI":"10.1021\/la970069t","article-title":"Functionalization of hydroxyl and carboxylic acid terminated self-assembled monolayers","volume":"13","author":"Hutt","year":"1997","journal-title":"Langmuir"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jim.2012.11.005","article-title":"Pairwise antibody footprinting using surface plasmon resonance technology to characterize human papillomavirus type 16 virus-like particles with direct anti-hpv antibody immobilization","volume":"388","author":"Towne","year":"2013","journal-title":"J. Immunol. Methods"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.bios.2004.07.037","article-title":"Enhanced performance of a surface plasmon resonance immunosensor for detecting ab-gad antibody based on the modified self-assembled monolayers","volume":"21","author":"Choi","year":"2005","journal-title":"Biosens. Bioelectron."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1021\/la001212c","article-title":"Molecular packing of semifluorinated alkanethiol self-assembled monolayers on gold: Influence of alkyl spacer length","volume":"17","author":"Tamada","year":"2001","journal-title":"Langmuir"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2256","DOI":"10.1039\/C1CS15166E","article-title":"Gold nanoparticles in biomedical applications: Recent advances and perspectives","volume":"41","author":"Dykman","year":"2012","journal-title":"Chem. Soc. Rev."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2739","DOI":"10.1021\/cr2001178","article-title":"Gold nanoparticles in chemical and biological sensing","volume":"112","author":"Saha","year":"2012","journal-title":"Chem. Rev."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.bios.2012.01.036","article-title":"Nanogold probe enhanced surface plasmon resonance immunosensor for improved detection of antibiotic residues","volume":"34","author":"Fernandez","year":"2012","journal-title":"Biosens. Bioelectron."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/13\/10\/12794\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:49:29Z","timestamp":1760219369000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/13\/10\/12794"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,25]]},"references-count":27,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2013,10]]}},"alternative-id":["s131012794"],"URL":"https:\/\/doi.org\/10.3390\/s131012794","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9,25]]}}}