{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T07:03:26Z","timestamp":1771052606678,"version":"3.50.1"},"reference-count":17,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2013,10,16]],"date-time":"2013-10-16T00:00:00Z","timestamp":1381881600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this work we present a surface plasmon resonance sensor based on enhanced optical transmission through sub-wavelength nanohole arrays. This technique is extremely sensitive to changes in the refractive index of the surrounding medium which result in a modulation of the transmitted light. The periodic gold nanohole array sensors were fabricated by high-throughput thermal nanoimprint lithography. Square periodic arrays with sub-wavelength hole diameters were obtained and characterized. Using solutions with known refractive index, the array sensitivities were obtained. Finally, protein absorption was monitored in real-time demonstrating the label-free biosensing capabilities of the fabricated devices.<\/jats:p>","DOI":"10.3390\/s131013960","type":"journal-article","created":{"date-parts":[[2013,10,16]],"date-time":"2013-10-16T12:20:47Z","timestamp":1381926047000},"page":"13960-13968","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Real-Time Label-Free Surface Plasmon Resonance  Biosensing with Gold Nanohole Arrays Fabricated by Nanoimprint Lithography"],"prefix":"10.3390","volume":"13","author":[{"given":"Josu","family":"Martinez-Perdiguero","sequence":"first","affiliation":[{"name":"CIC microGUNE, Arrasate-Mondrag\u00f3n 20500, Spain"}]},{"given":"Aritz","family":"Retolaza","sequence":"additional","affiliation":[{"name":"CIC microGUNE, Arrasate-Mondrag\u00f3n 20500, Spain"},{"name":"Micro and Nanofabrication Unit, IK4-Tekniker, Eibar 20600, Spain"}]},{"given":"Deitze","family":"Otaduy","sequence":"additional","affiliation":[{"name":"CIC microGUNE, Arrasate-Mondrag\u00f3n 20500, Spain"},{"name":"Micro and Nanofabrication Unit, IK4-Tekniker, Eibar 20600, Spain"}]},{"given":"Aritz","family":"Juarros","sequence":"additional","affiliation":[{"name":"CIC microGUNE, Arrasate-Mondrag\u00f3n 20500, Spain"},{"name":"Micro and Nanofabrication Unit, IK4-Tekniker, Eibar 20600, Spain"}]},{"given":"Santos","family":"Merino","sequence":"additional","affiliation":[{"name":"CIC microGUNE, Arrasate-Mondrag\u00f3n 20500, Spain"},{"name":"Micro and Nanofabrication Unit, IK4-Tekniker, Eibar 20600, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2013,10,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1002\/(SICI)1521-1878(199904)21:4<339::AID-BIES11>3.0.CO;2-C","article-title":"Instrumental biosensors: New perspectives for the analysis of biomolecular interactions","volume":"21","author":"Nice","year":"2009","journal-title":"BioEssays"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.3390\/s110201565","article-title":"Overview of the characteristics of micro- and nano-structured surface plasmon resonance sensors","volume":"11","author":"Roh","year":"2011","journal-title":"Sensors"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1038\/35570","article-title":"Extraordinary optical transmission through sub-wavelength hole arrays","volume":"391","author":"Ebbesen","year":"1998","journal-title":"Nature"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1743","DOI":"10.1364\/JOSAB.16.001743","article-title":"Surface-plasmon-enhanced transmission through hole arrays in Cr films","volume":"16","author":"Thio","year":"1999","journal-title":"J. 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