{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T08:39:46Z","timestamp":1765960786585,"version":"build-2065373602"},"reference-count":34,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2013,11,27]],"date-time":"2013-11-27T00:00:00Z","timestamp":1385510400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A versatile strategy for electrochemical determination of glycoalkaloids (GAs) was developed by using a carbon nanotubes-phenylboronic acid (CNTs-PBA) modified glassy carbon electrode. PBA reacts with \u03b1-solanine and \u03b1-chaconine to form a cyclic ester, which could be utilized to detect GAs. This method allowed GA detection from  1 \u03bcM to 28 \u03bcM and the detection limit was 0.3 \u03bcM. Affinity interaction of GAs and immobilized PBA caused an essential change of the peak current. The CNT-PBA modified electrodes were sensitive for detection of GAs, and the peak current values were in quite good agreement with those measured by the sensors.<\/jats:p>","DOI":"10.3390\/s131216234","type":"journal-article","created":{"date-parts":[[2013,11,27]],"date-time":"2013-11-27T14:43:20Z","timestamp":1385563400000},"page":"16234-16244","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Electrochemical Determination of Glycoalkaloids Using  a Carbon Nanotubes-Phenylboronic Acid Modified Glassy Carbon Electrode"],"prefix":"10.3390","volume":"13","author":[{"given":"Huiying","family":"Wang","sequence":"first","affiliation":[{"name":"College of Horticulture and Landscape, Hunan Agriculture University, Changsha 410128, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingyue","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Horticulture and Landscape, Hunan Agriculture University, Changsha 410128, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinxi","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Horticulture and Landscape, Hunan Agriculture University, Changsha 410128, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei","family":"Li","sequence":"additional","affiliation":[{"name":"College of Horticulture and Landscape, Hunan Agriculture University, Changsha 410128, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingyao","family":"Xiong","sequence":"additional","affiliation":[{"name":"College of Horticulture and Landscape, Hunan Agriculture University, Changsha 410128, China"},{"name":"The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences,  Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2013,11,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11540-008-9103-4","article-title":"Potato steroidal glycoalkaloids: Biosynthesis and genetic manipulation","volume":"52","author":"Ginzberg","year":"2009","journal-title":"Potato Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1021\/jf00015a011","article-title":"Distribution of glycoalkaloids in potato plants and commercial potato products","volume":"40","author":"Friedman","year":"1992","journal-title":"J. Agric. Food Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1021\/jf00033a010","article-title":"Kinetics of acid-catalyzed hydrolysis of carbohydrate groups of potato glycoalkaloids alpha-chaconine and alpha-solanine","volume":"41","author":"Friedman","year":"1993","journal-title":"J. Agric. Food Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1002\/jssc.200301685","article-title":"Enrichment of the glycoalkaloids alpha-solanine and alpha-chaconine from potato juice by adsorptive bubble separation using a ph gradient","volume":"27","author":"Backleh","year":"2004","journal-title":"J. Sep. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.lfs.2013.02.006","article-title":"Anti-inflammatory properties of potato glycoalkaloids in stimulated jurkat and raw 264.7 mouse macrophages","volume":"92","author":"Kenny","year":"2013","journal-title":"Life Sci."},{"key":"ref_6","first-page":"PD21","article-title":"Anticarcinogenic potential of solanum lycocarpum fruits glycoalkaloid extract in male wistar rats","volume":"78","author":"Munari","year":"2012","journal-title":"Planta Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1016\/j.fct.2005.11.007","article-title":"A-chaconine, a potato glycoalkaloid, induces apoptosis of ht-29 human colon cancer cells through caspase-3 activation and inhibition of erk 1\/2 phosphorylation","volume":"44","author":"Yang","year":"2006","journal-title":"Food Chem. Toxicol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2705","DOI":"10.1021\/jf9507405","article-title":"Improved high-performance liquid chromatographic method for the analysis of potato (solanum tuberosum) glycoalkaloids","volume":"44","author":"Edwards","year":"1996","journal-title":"J. Agric.Food Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"6949","DOI":"10.1021\/jf8006618","article-title":"Lc-ms analysis of solanidane glycoalkaloid diversity among tubers of four wild potato species and three cultivars (solanum tuberosum)","volume":"56","author":"Shakya","year":"2008","journal-title":"J. Agric.Food Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.talanta.2004.11.006","article-title":"Analysis of the potato glycoalkaloids by using of enzyme biosensor based on ph-isfets","volume":"66","author":"Soldatkin","year":"2005","journal-title":"Talanta"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1950","DOI":"10.1002\/elan.200603601","article-title":"Nonfaradaic impedance probing of potato glycoalkaloids interaction with butyrylcholinesterase immobilized onto gold electrode","volume":"18","author":"Benilova","year":"2006","journal-title":"Electroanalysis"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6162","DOI":"10.1021\/jf050620p","article-title":"Anticarcinogenic effects of glycoalkaloids from potatoes against human cervical, liver, lymphoma, and stomach cancer cells","volume":"53","author":"Friedman","year":"2005","journal-title":"J. Agric. Food Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.talanta.2007.05.050","article-title":"Direct electrochemistry and electrocatalysis of cytochrome c immobilized on gold nanoparticles\u2013chitosan\u2013carbon nanotubes-modified electrode","volume":"74","author":"Xiang","year":"2007","journal-title":"Talanta"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1021\/ac000967l","article-title":"Investigation of the electrochemical and electrocatalytic behavior of single-wall carbon nanotube film on a glassy carbon electrode","volume":"73","author":"Luo","year":"2001","journal-title":"Anal. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1016\/j.talanta.2005.05.030","article-title":"Composite film of carbon nanotubes and chitosan for preparation of amperometric hydrogen peroxide biosensor","volume":"68","author":"Qian","year":"2006","journal-title":"Talanta"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1016\/S1388-2481(02)00451-4","article-title":"Low-potential stable nadh detection at carbon-nanotube-modified glassy carbon electrodes","volume":"4","author":"Musameh","year":"2002","journal-title":"Electrochem. Commun."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1564","DOI":"10.1002\/elan.200603567","article-title":"Electrocatalytic dioxygen reduction at glassy carbon electrode modified with polyaniline grafted multiwall carbon nanotube film","volume":"18","author":"Manesh","year":"2006","journal-title":"Electroanalysis"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"11906","DOI":"10.1021\/ja053094r","article-title":"Single-walled carbon nanotube biosensors using aptamers as molecular recognition elements","volume":"127","author":"So","year":"2005","journal-title":"J. Am. Chem. Soc."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.elecom.2007.10.030","article-title":"Direct electron transfer of cytochrome c and its biosensor based on gold nanoparticles\/room temperature ionic liquid\/carbon nanotubes composite film","volume":"10","author":"Xiang","year":"2008","journal-title":"Electrochem. Commun."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1016\/j.snb.2009.06.039","article-title":"Direct electrochemistry of cytochrome c and biosensing for hydrogen peroxide on polyaniline grafted multi-walled carbon nanotube electrode","volume":"141","author":"Lee","year":"2009","journal-title":"Sens. Actuators B: Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1016\/j.msec.2011.05.007","article-title":"Electrochemical and optical sugar sensors based on phenylboronic acid and its derivatives","volume":"31","author":"Egawa","year":"2011","journal-title":"Mater. Sci. Eng.: C."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"12022","DOI":"10.1021\/ja902964m","article-title":"Noninvasive sialic acid detection at cell membrane by using phenylboronic acid modified self-assembled monolayer gold electrode","volume":"131","author":"Matsumoto","year":"2009","journal-title":"J. Am. Chem. Soc."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1016\/j.elecom.2005.08.021","article-title":"Phenylboronic acid self-assembled layer on glassy carbon electrode for recognition of glycoprotein peroxidase","volume":"7","author":"Liu","year":"2005","journal-title":"Electrochem. Commun."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5281","DOI":"10.1021\/ac0006526","article-title":"Surface-grafted molecularly imprinted polymers for protein recognition","volume":"73","author":"Bossi","year":"2001","journal-title":"Anal. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1016\/S0956-5663(01)00187-7","article-title":"Detection of HbA(1c) by boronate affinity immunoassay using bacterial magnetic particles","volume":"16","author":"Tanaka","year":"2001","journal-title":"Biosens. Bioelectron."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.aca.2004.08.059","article-title":"Quartz crystal biosensor for detection of sugars and glycated hemoglobin","volume":"530","year":"2005","journal-title":"Anal. Chim. Acta."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2322","DOI":"10.1016\/j.bios.2006.12.034","article-title":"Surface imprinted beads for the recognition of human serum albumin","volume":"22","author":"Bonini","year":"2007","journal-title":"Biosens. Bioelectron."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1021\/jo01088a011","article-title":"Polyol complexes and structure of the benzeneboronate ion","volume":"24","author":"Lorand","year":"1959","journal-title":"J. Org. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.2116\/bunsekikagaku.55.1003","article-title":"Effects of poly(allylamine) on the sugar-binding properties of a phenylboronic acid-appended azo dye (in Japanese)","volume":"55","author":"Egawa","year":"2006","journal-title":"Bunseki Kagaku"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"8970","DOI":"10.1021\/ac101911a","article-title":"Assessment of glycoprotein interactions with 4-[(2-aminoethyl) carbamoyl] phenylboronic acid surfaces using surface plasmon resonance spectroscopy","volume":"82","author":"Soper","year":"2010","journal-title":"Anal. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.bios.2012.02.040","article-title":"Sensitive colorimetric visualization of dihydronicotinamide adenine dinucleotide based on anti-aggregation of gold nanoparticles via boronic acid\u2013diol binding","volume":"35","author":"Liu","year":"2012","journal-title":"Biosens. Bioelectron"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"8516","DOI":"10.1021\/nn204899m","article-title":"Fluorescent polymer nanoparticle for selective sensing of intracellular hydrogen peroxide","volume":"6","author":"Oh","year":"2012","journal-title":"ACS Nano"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"5229","DOI":"10.1039\/c002533j","article-title":"Photoluminescent polymer nanoparticles for label-free cellular imaging","volume":"46","author":"Lee","year":"2010","journal-title":"Chem. Commun."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3282","DOI":"10.1021\/la803215z","article-title":"A multifunctional thin film au electrode surface formed by consecutive electrochemical reduction of aryl diazonium salts","volume":"25","author":"Harper","year":"2009","journal-title":"Langmuir"}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/13\/12\/16234\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:50:55Z","timestamp":1760219455000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/13\/12\/16234"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,27]]},"references-count":34,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2013,12]]}},"alternative-id":["s131216234"],"URL":"https:\/\/doi.org\/10.3390\/s131216234","relation":{},"ISSN":["1424-8220"],"issn-type":[{"type":"electronic","value":"1424-8220"}],"subject":[],"published":{"date-parts":[[2013,11,27]]}}}