{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T05:23:19Z","timestamp":1771478599436,"version":"3.50.1"},"reference-count":41,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2014,2,20]],"date-time":"2014-02-20T00:00:00Z","timestamp":1392854400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The study compared the biosensing properties of laccase biosensors based on carbon nanofibers (CNFs) and copper\/carbon composite nanofibers (Cu\/CNFs). The two kinds of nanofibers were prepared by electrospinning and carbonization under the same conditions. Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy were employed to investigate the morphologies and structures of CNFs and Cu\/CNFs. The amperometric results indicated that the Cu\/CNFs\/laccase(Lac)\/Nafion\/glass carbon electrode (GCE) possessed reliable analytical performance for the detection of catechol. The sensitivity of the Cu\/CNFs\/Lac\/Nafion\/GCE reached 33.1 \u03bcA\/mM, larger than that of CNFs\/Lac\/Nafion\/GCE. Meanwhile, Cu\/CNFs\/Lac\/Nafion\/GCE had a wider linear range from 9.95 \u00d7 10\u22126 to 9.76 \u00d7 10\u22123 M and a lower detection limit of 1.18 \u03bcM than CNFs\/Lac\/Nafion\/GCE. Moreover, it exhibited a good repeatability, reproducibility, selectivity and long-term stability, revealing that electrospun Cu\/CNFs have great potential in biosensing.<\/jats:p>","DOI":"10.3390\/s140203543","type":"journal-article","created":{"date-parts":[[2014,2,20]],"date-time":"2014-02-20T11:08:24Z","timestamp":1392894504000},"page":"3543-3556","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":67,"title":["Laccase Biosensor Based on Electrospun Copper\/Carbon Composite Nanofibers for Catechol Detection"],"prefix":"10.3390","volume":"14","author":[{"given":"Jiapeng","family":"Fu","sequence":"first","affiliation":[{"name":"Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China"}]},{"given":"Hui","family":"Qiao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China"}]},{"given":"Dawei","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China"}]},{"given":"Lei","family":"Luo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China"}]},{"given":"Ke","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China"}]},{"given":"Qufu","family":"Wei","sequence":"additional","affiliation":[{"name":"Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China"}]}],"member":"1968","published-online":{"date-parts":[[2014,2,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1365\/s10337-005-0570-3","article-title":"Direct analysis of phenol, catechol and hydroquinone in human urine by coupled-column hplc with fluorimetric detection","volume":"62","author":"Marrubini","year":"2005","journal-title":"Chromatographia"},{"key":"ref_2","first-page":"480","article-title":"Simultaneous determination of mixed phenol, catechol, and quinol by double fourier transform filtering and second ratio spectra derivative spectrophotometry","volume":"20","author":"Liu","year":"2000","journal-title":"Spectrosc. 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