{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T03:28:53Z","timestamp":1778902133478,"version":"3.51.4"},"reference-count":16,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2014,4,25]],"date-time":"2014-04-25T00:00:00Z","timestamp":1398384000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>We present an electrochemical MIP sensor for tamoxifen (TAM)\u2014a nonsteroidal anti-estrogen\u2014which is based on the electropolymerisation of an  O-phenylenediamine\u2012resorcinol mixture directly on the electrode surface in the presence  of the template molecule. Up to now only \u201cbulk\u201d MIPs for TAM have been  described in literature, which are applied for separation in chromatography columns.  Electro-polymerisation of the monomers in the presence of TAM generated a film which completely suppressed the reduction of ferricyanide. Removal of the template gave a markedly increased ferricyanide signal, which was again suppressed after rebinding as expected for filling of the cavities by target binding. The decrease of the ferricyanide peak of the MIP electrode depended linearly on the TAM concentration between 1 and 100 nM. The TAM-imprinted electrode showed a 2.3 times higher recognition of the template molecule itself as compared to its metabolite 4-hydroxytamoxifen and no cross-reactivity with the anticancer drug doxorubucin was found. Measurements at +1.1 V caused a fouling of the electrode surface, whilst pretreatment of TAM with peroxide in presence of HRP generated an oxidation product which was reducible at 0 mV, thus circumventing the polymer formation and electrochemical interferences.<\/jats:p>","DOI":"10.3390\/s140507647","type":"journal-article","created":{"date-parts":[[2014,4,28]],"date-time":"2014-04-28T05:15:00Z","timestamp":1398662100000},"page":"7647-7654","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["The First Electrochemical MIP Sensor for Tamoxifen"],"prefix":"10.3390","volume":"14","author":[{"given":"Aysu","family":"Yarman","sequence":"first","affiliation":[{"name":"Fraunhofer Institute for Biomedical Engineering IBMT, Am M\u00fchlenberg 13, 14476 Potsdam, Germany"},{"name":"Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25,  14476 Potsdam, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frieder","family":"Scheller","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Biomedical Engineering IBMT, Am M\u00fchlenberg 13, 14476 Potsdam, Germany"},{"name":"Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25,  14476 Potsdam, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2014,4,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2495","DOI":"10.1021\/cr990099w","article-title":"Molecularly imprinted polymers and their use in biomimetic sensors","volume":"100","author":"Haupt","year":"2000","journal-title":"Chem. 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