{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T09:51:28Z","timestamp":1771926688010,"version":"3.50.1"},"reference-count":19,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2014,6,10]],"date-time":"2014-06-10T00:00:00Z","timestamp":1402358400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Objective: To establish an electrochemical immunosensor for the  determination of serum trypsin levels using a multiwall carbon nanotubes  (MWCNTs)-composite-modified electrode. Method: A MWCNT composite coated on the surface of bare gold electrodes was used for fixation of an anti-trypsin antibody. The assembly process and the performance indicators, including sensitivity, linear range of detection, anti-jamming performance, and stability, of the electrochemical immunosensor were examined by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Results: With optimized experimental conditions, the difference of the current value measured by differential pulse voltammetry (DPV) showed a linear relationship with the concentration of serum trypsin within 0.10\u2013100 ng\/mL. The detection limit for trypsin using this sensor was 0.002 ng\/mL. Conclusions: The electrochemical immunosensor built using the MWCNT-composite-modified electrode is simple to operate and has a fast response time, along with a wide linear range, high sensitivity, and accuracy, making it suitable for serum trypsin detection.<\/jats:p>","DOI":"10.3390\/s140610203","type":"journal-article","created":{"date-parts":[[2014,6,10]],"date-time":"2014-06-10T11:50:51Z","timestamp":1402401051000},"page":"10203-10212","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Application of an Electrochemical Immunosensor with  a MWCNT\/PDAA Modified Electrode for Detection of  Serum Trypsin"],"prefix":"10.3390","volume":"14","author":[{"given":"Qiang","family":"Yi","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, the 1st Affiliated Hospital, Fujian Medical University,  Fuzhou 350005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qicai","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, the 1st Affiliated Hospital, Fujian Medical University,  Fuzhou 350005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pathology, the 1st Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingquan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, College of Medical Technology and Engineering,  Fujian Medical University, Fuzhou 350005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guina","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, College of Medical Technology and Engineering,  Fujian Medical University, Fuzhou 350005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2014,6,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1038\/nrgastro.2009.177","article-title":"Epidemiology of pancreatic cancer: An overview","volume":"6","author":"Raimondi","year":"2009","journal-title":"Nat. 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