{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:30:49Z","timestamp":1762079449393,"version":"build-2065373602"},"reference-count":26,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2014,8,8]],"date-time":"2014-08-08T00:00:00Z","timestamp":1407456000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["Super Science Fellowship","Future Fellowship","Georgina Sweet Laureate Fellowship"],"award-info":[{"award-number":["Super Science Fellowship","Future Fellowship","Georgina Sweet Laureate Fellowship"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>We report a novel approach to genotyping single nucleotide polymorphisms (SNPs) using molecular beacons in conjunction with a suspended core optical fiber (SCF). Target DNA sequences corresponding to the wild- or mutant-type have been accurately recognized by immobilizing two different molecular beacons on the core of a SCF.  The two molecular beacons differ by one base in the loop-probe and utilize different fluorescent indicators. Single-color fluorescence enhancement was obtained when the immobilized SCFs were filled with a solution containing either wild-type or mutant-type sequence (homozygous sample), while filling the immobilized SCF with solution containing both wild- and mutant-type sequences resulted in dual-color fluorescence enhancement, indicating a heterozygous sample. The genotyping was realized amplification-free and with ultra low-volume for the required DNA solution (nano-liter). This is, to our knowledge, the first genotyping device based on the combination of optical fiber and molecular beacons.<\/jats:p>","DOI":"10.3390\/s140814488","type":"journal-article","created":{"date-parts":[[2014,8,8]],"date-time":"2014-08-08T13:18:55Z","timestamp":1407503935000},"page":"14488-14499","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Genotyping Single Nucleotide Polymorphisms Using  Different Molecular Beacon Multiplexed within  a Suspended Core Optical Fiber"],"prefix":"10.3390","volume":"14","author":[{"given":"Linh","family":"Nguyen","sequence":"first","affiliation":[{"name":"Institute for Photonics and Advanced Sensing (IPAS) and The ARC Centre for  Nanoscale Biophotonics, The University of Adelaide, Adelaide 5005, Australia"}]},{"given":"Sara","family":"Giannetti","sequence":"additional","affiliation":[{"name":"Information Engineering Department, University of Parma, Parma 43100, Italy"}]},{"given":"Stephen","family":"Warren-Smith","sequence":"additional","affiliation":[{"name":"Institute for Photonics and Advanced Sensing (IPAS) and The ARC Centre for  Nanoscale Biophotonics, The University of Adelaide, Adelaide 5005, Australia"}]},{"given":"Alan","family":"Cooper","sequence":"additional","affiliation":[{"name":"Australian Centre for Ancient DNA (ACAD), The University of Adelaide, Adelaide 5005, Australia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8026-0846","authenticated-orcid":false,"given":"Stefano","family":"Selleri","sequence":"additional","affiliation":[{"name":"Information Engineering Department, University of Parma, Parma 43100, Italy"}]},{"given":"Annamaria","family":"Cucinotta","sequence":"additional","affiliation":[{"name":"Information Engineering Department, University of Parma, Parma 43100, Italy"}]},{"given":"Tanya","family":"Monro","sequence":"additional","affiliation":[{"name":"Institute for Photonics and Advanced Sensing (IPAS) and The ARC Centre for  Nanoscale Biophotonics, The University of Adelaide, Adelaide 5005, Australia"}]}],"member":"1968","published-online":{"date-parts":[[2014,8,8]]},"reference":[{"key":"ref_1","unstructured":"Kwok, P.-Y. 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