{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T06:03:36Z","timestamp":1769925816451,"version":"3.49.0"},"reference-count":53,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2014,9,2]],"date-time":"2014-09-02T00:00:00Z","timestamp":1409616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Advancements in optical nanosensor development have enabled the design of sensors using synthetic molecular recognition elements through a recently developed method called Corona Phase Molecular Recognition (CoPhMoRe). The synthetic sensors resulting from these design principles are highly selective for specific analytes, and demonstrate remarkable stability for use under a variety of conditions. An essential element of nanosensor development hinges on the ability to understand the interface between nanoparticles and the associated corona phase surrounding the nanosensor, an environment outside of the range of traditional characterization tools, such as NMR. This review discusses the need for new strategies and instrumentation to study the nanoparticle corona, operating in both in vitro and in vivo environments. Approaches to instrumentation must have the capacity to concurrently monitor nanosensor operation and the molecular changes in the corona phase. A detailed overview of new tools for the understanding of CoPhMoRe mechanisms is provided for future applications.<\/jats:p>","DOI":"10.3390\/s140916196","type":"journal-article","created":{"date-parts":[[2014,9,2]],"date-time":"2014-09-02T10:12:38Z","timestamp":1409652758000},"page":"16196-16211","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Experimental Tools to Study Molecular Recognition within the Nanoparticle Corona"],"prefix":"10.3390","volume":"14","author":[{"given":"Markita","family":"Landry","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering, Massachusetts Institute of Technology,  77 Massachusetts Ave. Cambridge, MA 02139, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastian","family":"Kruss","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Massachusetts Institute of Technology,  77 Massachusetts Ave. Cambridge, MA 02139, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin","family":"Nelson","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Massachusetts Institute of Technology,  77 Massachusetts Ave. Cambridge, MA 02139, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gili","family":"Bisker","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Massachusetts Institute of Technology,  77 Massachusetts Ave. Cambridge, MA 02139, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Iverson","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Massachusetts Institute of Technology,  77 Massachusetts Ave. Cambridge, MA 02139, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nigel","family":"Reuel","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Massachusetts Institute of Technology,  77 Massachusetts Ave. Cambridge, MA 02139, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Strano","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Massachusetts Institute of Technology,  77 Massachusetts Ave. Cambridge, MA 02139, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2014,9,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1021\/cm402895e","article-title":"Low Dimensional Carbon Materials for Applications in Mass and Energy Transport","volume":"26","author":"Wang","year":"2014","journal-title":"Chem. Mater."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.aca.2010.01.009","article-title":"Review: Carbon nanotube based electrochemical sensors for biomolecules","volume":"662","author":"Jacobs","year":"2010","journal-title":"Anal. Chim. Acta"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1038\/nature02388","article-title":"Designing materials for biology and medicine","volume":"428","author":"Langer","year":"2004","journal-title":"Nature"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1126\/science.1125559","article-title":"Oligonucleotide-modified gold nanoparticles for intracellular gene regulation","volume":"312","author":"Rosi","year":"2006","journal-title":"Science"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"8138","DOI":"10.1002\/anie.200603138","article-title":"Reversible control of carbon nanotube aggregation for a glucose affinity sensor","volume":"45","author":"Barone","year":"2006","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1002\/cssc.201100070","article-title":"Near-infrared fluorescent sensors based on single-walled carbon nanotubes for life sciences applications","volume":"4","author":"Boghossian","year":"2011","journal-title":"ChemSusChem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"17923","DOI":"10.1021\/ja2074938","article-title":"Transduction of glycan-lectin binding using near-infrared fluorescent single-walled carbon nanotubes for glycan profiling","volume":"133","author":"Reuel","year":"2011","journal-title":"J. Am. Chem. Soc."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1021\/ja1084942","article-title":"Single Molecule Detection of Nitric Oxide Enabled by d(AT)(15) DNA Adsorbed to Near Infrared Fluorescent Single-Walled Carbon Nanotubes","volume":"133","author":"Zhang","year":"2011","journal-title":"J. Am. Chem. Soc."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1021\/nl051899r","article-title":"Peptide-mediated formation of single-wall carbon nanotube composites","volume":"6","author":"Pender","year":"2006","journal-title":"Nano Lett."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"10648","DOI":"10.1021\/ja801720c","article-title":"Atomic force microscopy studies of DNA-wrapped carbon nanotube structure and binding to quantum dots","volume":"130","author":"Campbell","year":"2008","journal-title":"J. Am. Chem. Soc."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Sapsford, K.E., Tyner, K.M., Dair, B.J., Deschamps, J.R., and Medintz, I.L. (2011). Analyzing nanomaterial bioconjugates: A review of current and emerging purification and characterization techniques. Anal. Chem., 4453\u20134488.","DOI":"10.1021\/ac200853a"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1038\/nmat2442","article-title":"Understanding biophysicochemical interactions at the nano-bio interface","volume":"8","author":"Nel","year":"2009","journal-title":"Nat. Mater."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1146\/annurev.pharmtox.010909.105819","article-title":"Close encounters of the small kind: Adverse effects of man-made materials interfacing with the nano-cosmos of biological systems","volume":"50","author":"Shvedova","year":"2010","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1002\/smll.200700217","article-title":"Assessing the effect of surface chemistry on gold nanorod uptake, toxicity, and gene expression in mammalian cells","volume":"4","author":"Hauck","year":"2008","journal-title":"Small"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.cell.2006.05.046","article-title":"Single-molecule analysis of dynein processivity and stepping behavior","volume":"126","author":"Yildiz","year":"2006","journal-title":"Cell"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1126\/science.1108408","article-title":"Kinesin and dynein move a peroxisome in vivo: A tug-of-war or coordinated movement?","volume":"308","author":"Kural","year":"2005","journal-title":"Science"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1038\/nature10016","article-title":"Probing cellular protein complexes using single-molecule pull-down","volume":"473","author":"Jain","year":"2011","journal-title":"Nature"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1021\/nl050366f","article-title":"Versatile visualization of individual single-walled carbon nanotubes with near-infrared fluorescence microscopy","volume":"5","author":"Tsyboulski","year":"2005","journal-title":"Nano Lett."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1002\/cyto.a.20687","article-title":"Supercontinuum white light lasers for flow cytometry","volume":"75","author":"Telford","year":"2009","journal-title":"Cytometry A"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1038\/nmeth.1208","article-title":"A practical guide to single-molecule FRET","volume":"5","author":"Roy","year":"2008","journal-title":"Nat. Methods"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.cplett.2004.01.116","article-title":"Fluorescence spectroscopy of single-walled carbon nanotubes synthesized from alcohol","volume":"387","author":"Miyauchi","year":"2004","journal-title":"Chem. Phys. Lett."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1111\/j.1365-2818.2012.03603.x","article-title":"Use of a white light supercontinuum laser for confocal interference-reflection microscopy","volume":"246","author":"Chiu","year":"2012","journal-title":"J. Microsc."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"032143","DOI":"10.1063\/1.3631661","article-title":"Supercontinuum ultra wide range confocal microscope for reflectance spectroscopy of living matter and material science surfaces","volume":"1","author":"Selci","year":"2011","journal-title":"AIP Adv."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1038\/nnano.2013.236","article-title":"Molecular recognition using corona complex made of artificial polymers adsorbed on carbon nanotubes","volume":"8","author":"Zhang","year":"2013","journal-title":"Nat. Nanotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/S0952-7915(99)00013-8","article-title":"Recombinant antibody constructs in cancer therapy","volume":"11","author":"Hudson","year":"1999","journal-title":"Curr. Opin. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/S0958-1669(02)00348-8","article-title":"Monoclonal antibodies as therapeutics in oncology","volume":"13","author":"Trikha","year":"2002","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"8351","DOI":"10.1021\/ja800604z","article-title":"Carbon nanotube-quenched fluorescent oligonucleotides: Probes that fluoresce upon hybridization","volume":"130","author":"Yang","year":"2008","journal-title":"J. Am. Chem. Soc."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1021\/cn4000956","article-title":"Visualizing Neurotransmitter Secretion at Individual Synapses","volume":"4","author":"Sames","year":"2013","journal-title":"ACS Chem. Neurosci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/s00441-004-0956-9","article-title":"Stages in the development of Parkinson's disease-related pathology","volume":"318","author":"Braak","year":"2004","journal-title":"Cell Tissue Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"7242","DOI":"10.1021\/ac200666c","article-title":"Electroanalytical eavesdropping on single cell communication","volume":"83","author":"Kim","year":"2011","journal-title":"Anal. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.1073\/pnas.0606636104","article-title":"The optimal height of the synaptic cleft","volume":"104","author":"Savtchenko","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1021\/ja410433b","article-title":"Neurotransmitter detection using corona phase molecular recognition on fluorescent single-walled carbon nanotube sensors","volume":"136","author":"Kruss","year":"2014","journal-title":"J. Am. Chem. Soc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1038\/nmat3890","article-title":"Plant nanobionics approach to augment photosynthesis and biochemical sensing","volume":"13","author":"Giraldo","year":"2014","journal-title":"Nat. Mater."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1038\/nnano.2013.222","article-title":"In vivo biosensing via tissue-localizable near-infrared-fluorescent single-walled carbon nanotubes","volume":"8","author":"Iverson","year":"2013","journal-title":"Nat. Nanotechnol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Bisker, G., Iverson, N.M., Ahn, J., and Strano, M.S. (2014). A Pharmacokinetic Model of a Tissue Implantable Insulin Sensor. Adv. Healthc. Mater.","DOI":"10.1002\/adhm.201400264"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1021\/jp026731y","article-title":"The Optical Properties of Metal Nanoparticles: The Influence of Size, Shape, and Dielectric Environment","volume":"107","author":"Kelly","year":"2002","journal-title":"J. Phys. Chem. B"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2171","DOI":"10.1002\/smll.201202243","article-title":"Comparison of nanotube-protein corona composition in cell culture media","volume":"9","author":"Shannahan","year":"2013","journal-title":"Small"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1126\/science.1091911","article-title":"Structure-based carbon nanotube sorting by sequence-dependent DNA assembly","volume":"302","author":"Zheng","year":"2003","journal-title":"Science"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2051","DOI":"10.1021\/nn100257h","article-title":"Two-dimensional diffusion-ordered NMR spectroscopy as a tool for monitoring functionalized carbon nanotube purification and composition","volume":"4","author":"Marega","year":"2010","journal-title":"ACS Nano"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"9004","DOI":"10.1021\/ja062095w","article-title":"Racemic single-walled carbon nanotubes exhibit circular dichroism when wrapped with DNA","volume":"128","author":"Dukovic","year":"2006","journal-title":"J. Am. Chem. Soc."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"223114","DOI":"10.1063\/1.2745228","article-title":"Solvatochromism in single-walled carbon nanotubes","volume":"90","author":"Choi","year":"2007","journal-title":"Appl. Phys. Lett."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1379","DOI":"10.1021\/nl034524j","article-title":"Individually suspended single-walled carbon nanotubes in various surfactants","volume":"3","author":"Moore","year":"2003","journal-title":"Nano Lett."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1002\/adhm.201300033","article-title":"A Rapid, Direct, Quantitative, and Label-Free Detector of Cardiac Biomarker Troponin T Using Near-Infrared Fluorescent Single-Walled Carbon Nanotube Sensors","volume":"3","author":"Zhang","year":"2013","journal-title":"Adv. Healthc. Mater."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1815","DOI":"10.1016\/j.polymer.2007.02.004","article-title":"\u201cSmart\u201d nanoparticles: Preparation, characterization and applications","volume":"48","author":"Ballauff","year":"2007","journal-title":"Polymer"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"11025","DOI":"10.1021\/la302307m","article-title":"Small-Angle Neutron Scattering from Aqueous Dispersions of Single-Walled Carbon Nanotubes with Pluronic F127 and Poly(vinylpyrrolidone)","volume":"28","author":"Granite","year":"2012","journal-title":"Langmuir"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.cocis.2012.10.003","article-title":"Cryo-TEM of soft molecular assemblies","volume":"17","author":"Danino","year":"2012","journal-title":"Curr. Opin. Colloid Interface Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5603\/FHC.2014.0001","article-title":"Closer to the native state. Critical evaluation of cryo-techniques for Transmission Electron Microscopy: Preparation of biological samples","volume":"52","author":"Mielanczyk","year":"2014","journal-title":"Folia Histochem. Cytobiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.cis.2014.01.007","article-title":"Progress in the direct structural characterization of fibrous amphiphilic supramolecular assemblies in solution by transmission electron microscopic techniques","volume":"208","author":"Berlepsch","year":"2014","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.1021\/nl048969z","article-title":"Dispersing Single-Walled Carbon Nanotubes with Surfactants: A Small Angle Neutron Scattering Study","volume":"4","author":"Wang","year":"2004","journal-title":"Nano Lett."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"045401","DOI":"10.1103\/PhysRevB.73.045401","article-title":"Optical circular dichroism of single-wall carbon nanotubes","volume":"73","author":"Noguez","year":"2006","journal-title":"Phys. Rev. B"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"205402","DOI":"10.1103\/PhysRevB.69.205402","article-title":"Interband optical transitions in left- and right-handed single-wall carbon nanotubes","volume":"69","author":"Samsonidze","year":"2004","journal-title":"Phys. Rev. B"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"22725","DOI":"10.1021\/jp053025z","article-title":"Optically Active Polymer Carbon Nanotube Composite","volume":"109","author":"Panhuis","year":"2005","journal-title":"J. Phys. Chem. B"},{"key":"ref_53","first-page":"011005","article-title":"Giant Circular Dichroism in Individual Carbon Nanotubes Induced by Extrinsic Chirality","volume":"4","author":"Yokoyama","year":"2014","journal-title":"Phys. Rev. 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