{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T02:25:02Z","timestamp":1768271102730,"version":"3.49.0"},"reference-count":31,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2014,9,10]],"date-time":"2014-09-10T00:00:00Z","timestamp":1410307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A rapid, simple, and sensitive immunochromatographic test strip has been developed for testing residues of ciprofloxacin (CIP). A specific and sensitive  monoclonal antibody (mAb) for CIP was generated by immunizing BALB\/c mice with well-characterized CIP-Keyhole limpet haemocyanin. Under the optimized conditions, the cut-off limits of test strips for CIP were found to be 5 ng\/mL in phosphate-buffered saline and 2.5 ng\/mL in milk samples. Each test can be evaluated within 3 min. The  cross-reactivities of the CIP test strip to enrofloxacin (ENR), norfloxacin (NOR), nadifloxacin (NDF), danofloxacin (DANO), pefloxacin (PEX), lomefloxacin (LOME), enoxacin (ENO), and sarafloxacin (SAR) were 71.4%, 71.4%, 66%, 50%, 33%, 20%, 12.5%, and 6.25%, respectively. The data indicate that the method is sensitive, specific, and has the advantages of simplicity and speed, therefore, this test strip is a useful screening method for the detection of CIP residues in milk samples.<\/jats:p>","DOI":"10.3390\/s140916785","type":"journal-article","created":{"date-parts":[[2014,9,11]],"date-time":"2014-09-11T02:22:24Z","timestamp":1410402144000},"page":"16785-16798","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":76,"title":["Development of an Immunochromatographic Strip Test for Rapid Detection of Ciprofloxacin in Milk Samples"],"prefix":"10.3390","volume":"14","author":[{"given":"Liqiang","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Lab of Food Science and Technology, School of Food Science and Technology,  Jiangnan University, Wuxi 214122, China"}]},{"given":"Liju","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Lab of Food Science and Technology, School of Food Science and Technology,  Jiangnan University, Wuxi 214122, China"}]},{"given":"Steven","family":"Suryoprabowo","sequence":"additional","affiliation":[{"name":"State Key Lab of Food Science and Technology, School of Food Science and Technology,  Jiangnan University, Wuxi 214122, China"}]},{"given":"Juan","family":"Peng","sequence":"additional","affiliation":[{"name":"State Key Lab of Food Science and Technology, School of Food Science and Technology,  Jiangnan University, Wuxi 214122, China"}]},{"given":"Hua","family":"Kuang","sequence":"additional","affiliation":[{"name":"State Key Lab of Food Science and Technology, School of Food Science and Technology,  Jiangnan University, Wuxi 214122, China"}]},{"given":"Chuanlai","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Lab of Food Science and Technology, School of Food Science and Technology,  Jiangnan University, Wuxi 214122, China"}]}],"member":"1968","published-online":{"date-parts":[[2014,9,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5469","DOI":"10.1021\/jf800274f","article-title":"Development of an immunochromatography strip for the rapid detection of 12 fluoroquinolones in chicken muscle and liver","volume":"56","author":"Zhu","year":"2008","journal-title":"J. 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