{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T13:06:29Z","timestamp":1780491989971,"version":"3.54.1"},"reference-count":89,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2014,9,12]],"date-time":"2014-09-12T00:00:00Z","timestamp":1410480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this paper, we review recent advances in on-chip sensors integrated with microfluidics for biological applications. Since the 1990s, much research has concentrated on developing a sensing system using optical phenomena such as surface plasmon resonance (SPR) and surface-enhanced Raman scattering (SERS) to improve the sensitivity of the device. The sensing performance can be significantly enhanced with the use of microfluidic chips to provide effective liquid manipulation and greater flexibility. We describe an optical image sensor with a simpler platform for better performance over a larger field of view (FOV) and greater depth of field (DOF). As a new trend, we review consumer electronics such as smart phones, tablets, Google glasses, etc. which are being incorporated in point-of-care (POC) testing systems. In addition, we discuss in detail the current optical sensing system integrated with a microfluidic chip.<\/jats:p>","DOI":"10.3390\/s140917008","type":"journal-article","created":{"date-parts":[[2014,9,12]],"date-time":"2014-09-12T10:25:43Z","timestamp":1410517543000},"page":"17008-17036","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["Various On-Chip Sensors with Microfluidics for  Biological Applications"],"prefix":"10.3390","volume":"14","author":[{"given":"Hun","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Electrical Engineering, University at Buffalo, State University of New York  (SUNY at Buffalo), Buffalo, NY 14260, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6386-1815","authenticated-orcid":false,"given":"Linfeng","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Electrical Engineering, University at Buffalo, State University of New York  (SUNY at Buffalo), Buffalo, NY 14260, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6515-5543","authenticated-orcid":false,"given":"Domin","family":"Koh","sequence":"additional","affiliation":[{"name":"Department of Electrical Engineering, University at Buffalo, State University of New York  (SUNY at Buffalo), Buffalo, NY 14260, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikhila","family":"Nyayapathi","sequence":"additional","affiliation":[{"name":"Department of Electrical Engineering, University at Buffalo, State University of New York  (SUNY at Buffalo), Buffalo, NY 14260, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0078-6683","authenticated-orcid":false,"given":"Kwang","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Electrical Engineering, University at Buffalo, State University of New York  (SUNY at Buffalo), Buffalo, NY 14260, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2014,9,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1038\/nature05059","article-title":"Scaling and the design of miniaturized chemical-analysis systems","volume":"442","author":"Janasek","year":"2006","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/S1367-5931(03)00050-4","article-title":"Microfluidic combinatorial chemistry","volume":"7","author":"Watts","year":"2003","journal-title":"Curr. Opin. Chem. Biol."},{"key":"ref_3","first-page":"798","article-title":"Microfluidic systems for high-throughput and combinatorial chemistry","volume":"7","author":"Cullen","year":"2004","journal-title":"Curr. Opin. Drug Disc."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1002\/ceat.200407128","article-title":"Microreactor technology: A revolution for the fine chemical and pharmaceutical industries?","volume":"28","author":"Roberge","year":"2005","journal-title":"Chem. Eng. Technol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1126\/science.1118919","article-title":"Multistep synthesis of a radiolabeled imaging probe using integrated microfluidics","volume":"310","author":"Lee","year":"2005","journal-title":"Science"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1548","DOI":"10.1039\/b820321k","article-title":"A fully automated immunoassay from whole blood on a disc","volume":"9","author":"Lee","year":"2009","journal-title":"Lab Chip"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.1039\/b818430e","article-title":"Integrated microfluidic immunoassay for the rapid determination of clenbuterol","volume":"9","author":"Kong","year":"2009","journal-title":"Lab Chip"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1039\/C0LC00204F","article-title":"Lab-on-a-chip based immunosensor principles and technologies for the detection of cardiac biomarkers: A review","volume":"11","author":"Mohammed","year":"2011","journal-title":"Lab Chip"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Lee, H., Kim, H.J., Park, J.H., Jeong, D.H., and Lee, S.K. (2010). Effects of surface density and size of gold nanoparticles in a fiber-optic localized surface plasmon resonance sensor and its application to peptide detection. Meas. Sci. Technol., 2.","DOI":"10.1088\/0957-0233\/21\/8\/085805"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3611","DOI":"10.1039\/c2lc40498b","article-title":"Joining plasmonics with microfluidics: From convenience to inevitability","volume":"12","author":"Kim","year":"2012","journal-title":"Lab Chip"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1155\/2003\/372913","article-title":"Surface plasmon resonance: Principles, methods and applications in biomedical sciences","volume":"17","author":"Englebienne","year":"2003","journal-title":"Spectrosc.-Int. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s11468-007-9044-9","article-title":"Importance of metal-adsorbate interactions for the surface-enhanced raman scattering of molecules adsorbed on plasmonic nanoparticles","volume":"2","author":"Guerrini","year":"2007","journal-title":"Plasmonics"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"7207","DOI":"10.1063\/1.1450542","article-title":"The role of charge-transfer states of the metal-adsorbate complex in surface-enhanced raman scattering","volume":"116","author":"Arenas","year":"2002","journal-title":"J. Chem. Phys."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"16592","DOI":"10.1021\/ja803556k","article-title":"Observing metal-catalyzed chemical reactions in situ using surface-enhanced raman spectroscopy on pd-au nanoshells","volume":"130","author":"Heck","year":"2008","journal-title":"J. Am. Chem. Soc."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"19302","DOI":"10.1021\/ja208298q","article-title":"Synthesis of bifunctional au\/pt\/au core\/shell nanoraspberries for in situ sers monitoring of platinum-catalyzed reactions","volume":"133","author":"Xie","year":"2011","journal-title":"J. Am. Chem. Soc."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"7592","DOI":"10.1002\/anie.201203526","article-title":"Characterizing the kinetics of nanoparticle-catalyzed reactions by surface-enhanced raman scattering","volume":"51","author":"Joseph","year":"2012","journal-title":"Angew. Chem. Int. Edit."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2166","DOI":"10.1021\/ac026308l","article-title":"On-line monitoring of airborne chemistry in levitated nanodroplets: In situ synthesis and application of sers-active ag-sols for trace analysis by ft-raman spectroscopy","volume":"75","author":"Leopold","year":"2003","journal-title":"Anal. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1039\/b708459p","article-title":"SERS\u2014A single-molecule and nanoscale tool for bioanalytics","volume":"37","author":"Kneipp","year":"2008","journal-title":"Chem. Soc. Rev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.biomaterials.2011.09.032","article-title":"Nanoprobes for in vitro diagnostics of cancer and infectious diseases","volume":"33","author":"Chi","year":"2012","journal-title":"Biomaterials"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"203103","DOI":"10.1063\/1.3429605","article-title":"Liquid cell with plasmon lenses for surface enhanced raman spectroscopy","volume":"96","author":"Bahns","year":"2010","journal-title":"Appl. Phys. Lett."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1039\/C2LC90127G","article-title":"Optofluidic imaging: Now and beyond","volume":"13","author":"Zhao","year":"2013","journal-title":"Lab Chip"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1039\/C2LC40864C","article-title":"Optical imaging techniques for point-of-care diagnostics","volume":"13","author":"Zhu","year":"2013","journal-title":"Lab Chip"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2028","DOI":"10.1039\/c3lc50222h","article-title":"Toward giga-pixel nanoscopy on a chip: A computational wide-field look at the nano-scale without the use of lenses","volume":"13","author":"McLeod","year":"2013","journal-title":"Lab Chip"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1038\/nmeth.1476","article-title":"Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy","volume":"7","author":"Keller","year":"2010","journal-title":"Nat. Methods"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1642","DOI":"10.1126\/science.1127344","article-title":"Imaging intracellular fluorescent proteins at nanometer resolution","volume":"313","author":"Betzig","year":"2006","journal-title":"Science"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/S0140-6736(98)06318-1","article-title":"Feasibility and efficacy of routine pcr screening of blood donations for hepatitis c virus, hepatitis b virus, and hiv-1 in a blood-bank setting","volume":"353","author":"Roth","year":"1999","journal-title":"Lancet"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1002\/cyto.a.20662","article-title":"Single-cell-based image analysis of high-throughput cell array screens for quantification of viral infection","volume":"75A","author":"Matula","year":"2009","journal-title":"Cytom. Part A"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3552","DOI":"10.1039\/c2lc40509a","article-title":"Optofluidic detection for cellular phenotyping","volume":"12","author":"Tung","year":"2012","journal-title":"Lab Chip"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3543","DOI":"10.1039\/c2lc40467b","article-title":"Optofluidic devices and applications in photonics, sensing and imaging","volume":"12","author":"Pang","year":"2012","journal-title":"Lab Chip"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3566","DOI":"10.1039\/c2lc40517b","article-title":"Optical imaging techniques in microfluidics and their applications","volume":"12","author":"Wu","year":"2012","journal-title":"Lab Chip"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1039\/c000453g","article-title":"Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications","volume":"10","author":"Mudanyali","year":"2010","journal-title":"Lab Chip"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Pushkarsky, I., Lyb, Y.B., Weaver, W., Su, T.W., Mudanyali, O., Ozcan, A., and Di Carlo, D. (2014). Automated single-cell motility analysis on a chip using lensfree microscopy. Sci. Rep., 4.","DOI":"10.1038\/srep04717"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2222","DOI":"10.1039\/c1lc20127a","article-title":"Field-portable lensfree tomographic microscope","volume":"11","author":"Isikman","year":"2011","journal-title":"Lab Chip"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1038\/nphoton.2012.337","article-title":"Wide-field optical detection of nanoparticles using on-chip microscopy and self-assembled nanolenses","volume":"7","author":"Mudanyali","year":"2013","journal-title":"Nat. Photonics"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"17378","DOI":"10.1364\/OE.19.017378","article-title":"Lensfree super-resolution holographic microscopy using wetting films on a chip","volume":"19","author":"Mudanyali","year":"2011","journal-title":"Opt. Express"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1039\/b923554j","article-title":"High-throughput fluorescence detection using an integrated zone-plate array","volume":"10","author":"Schonbrun","year":"2010","journal-title":"Lab Chip"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"4968","DOI":"10.1039\/c2lc40894e","article-title":"High-throughput screening of large volumes of whole blood using structured illumination and fluorescent on-chip imaging","volume":"12","author":"Arpali","year":"2012","journal-title":"Lab Chip"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"10510","DOI":"10.1364\/OE.18.010510","article-title":"Lensless wide-field fluorescent imaging on a chip using compressive decoding of sparse objects","volume":"18","author":"Coskun","year":"2010","journal-title":"Opt. Express"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"5011","DOI":"10.1039\/c2an35729a","article-title":"Modeling and design of micromachined optical soller collimators for lensless ccd-based fluorometry","volume":"137","author":"Balsam","year":"2012","journal-title":"Analyst"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1039\/c0lc00431f","article-title":"Lensless CCD-based fluorometer using a micromachined optical soller collimator","volume":"11","author":"Balsam","year":"2011","journal-title":"Lab Chip"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1177\/2211068211426695","article-title":"Lens-free imaging for biological applications","volume":"17","author":"Kim","year":"2012","journal-title":"Jala-J. Lab Autom."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1021\/cr068107d","article-title":"Surface plasmon resonance sensors for detection of chemical and biological species","volume":"108","author":"Homola","year":"2008","journal-title":"Chem. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"4755","DOI":"10.1039\/c3lc50863c","article-title":"Plasmonic interferometric sensor arrays for high-performance label-free biomolecular detection","volume":"13","author":"Gao","year":"2013","journal-title":"Lab Chip"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1038\/nmat2162","article-title":"Biosensing with plasmonic nanosensors","volume":"7","author":"Anker","year":"2008","journal-title":"Nat. Mater."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"4439","DOI":"10.1002\/adfm.201200955","article-title":"Linewidth-optimized extraordinary optical transmission in water with template-stripped metallic nanohole arrays","volume":"22","author":"Lee","year":"2012","journal-title":"Adv. Funct. Mater."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1021\/nl902621d","article-title":"Planar metamaterial analogue of electromagnetically induced transparency for plasmonic sensing","volume":"10","author":"Liu","year":"2010","journal-title":"Nano Lett."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1021\/nl102206x","article-title":"Programmable soft lithography: Solvent-assisted nanoscale embossing","volume":"11","author":"Lee","year":"2011","journal-title":"Nano Lett."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1039\/c2lc41184a","article-title":"Enhancement of affinity-based biosensors: Effect of sensing chamber geometry on sensitivity","volume":"13","author":"Lynn","year":"2013","journal-title":"Lab Chip"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1685","DOI":"10.1002\/adma.200400271","article-title":"Exploitation of localized surface plasmon resonance","volume":"16","author":"Hutter","year":"2004","journal-title":"Adv. Mater."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1021\/jp0361327","article-title":"A nanoscale optical biosensor: The long range distance dependence of the localized surface plasmon resonance of noble metal nanoparticles","volume":"108","author":"Haes","year":"2004","journal-title":"J. Phys. Chem. B"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1007\/s00216-004-2708-9","article-title":"A unified view of propagating and localized surface plasmon resonance biosensors","volume":"379","author":"Haes","year":"2004","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1021\/nl204596h","article-title":"Probing dynamically tunable localized surface plasmon resonances of film-coupled nanoparticles by evanescent wave excitation","volume":"12","author":"Mock","year":"2012","journal-title":"Nano Lett."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1021\/nl200135r","article-title":"Substrate-induced Fano resonances of a plasmonic: Nanocube: A route to increased-sensitivity localized surface plasmon resonance sensors revealed","volume":"11","author":"Zhang","year":"2011","journal-title":"Nano Lett."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2605","DOI":"10.1021\/ac200432c","article-title":"Nanoarray-based biomolecular detection using individual au nanoparticles with minimized localized surface plasmon resonance variations","volume":"83","author":"Guo","year":"2011","journal-title":"Anal. Chem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3299","DOI":"10.1039\/c1lc20468h","article-title":"In situ assembly, regeneration and plasmonic immunosensing of a au nanorod monolayer in a closed-surface flow channel","volume":"11","author":"Guo","year":"2011","journal-title":"Lab Chip"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1021\/nl034372s","article-title":"Single silver nanoparticles as real-time optical sensors with zeptomole sensitivity","volume":"3","author":"McFarland","year":"2003","journal-title":"Nano Lett."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1021\/cr0300789","article-title":"Self-assembled monolayers of thiolates on metals as a form of nanotechnology","volume":"105","author":"Love","year":"2005","journal-title":"Chem. Rev."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1038\/nbt1100","article-title":"A molecular ruler based on plasmon coupling of single gold and silver nanoparticles","volume":"23","author":"Sonnichsen","year":"2005","journal-title":"Nat. Biotechnol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"3901","DOI":"10.1039\/c2lc40388a","article-title":"Enantioselective analysis of melagatran via an lspr biosensor integrated with a microfluidic chip","volume":"12","author":"Guo","year":"2012","journal-title":"Lab Chip"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1039\/c3lc51220g","article-title":"Sensitive determination of reactive oxygen species in cigarette smoke using microchip electrophoresis-localized surface plasmon resonance enhanced fluorescence detection","volume":"14","author":"Wang","year":"2014","journal-title":"Lab Chip"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1038\/nchem.1582","article-title":"Single-molecule fluorescence assembling nanoantennas","volume":"5","author":"Cosa","year":"2013","journal-title":"Nat. Chem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1002\/anie.201004806","article-title":"Surface-enhanced fluorescence with shell-isolated nanoparticles (shinef)","volume":"50","author":"Guerrero","year":"2011","journal-title":"Angew. Chem. Int. Edit."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"8057","DOI":"10.1021\/ac2012976","article-title":"Advances in surface plasmon resonance sensing with nanoparticles and thin films: Nanomaterials, surface chemistry, and hybrid plasmonic techniques","volume":"83","author":"Bolduc","year":"2011","journal-title":"Anal. Chem."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"5728","DOI":"10.1021\/cr4000623","article-title":"Advances in plasmonic technologies for point of care applications","volume":"114","author":"Tokel","year":"2014","journal-title":"Chem. Rev."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1039\/b920589f","article-title":"Parallel microfluidic surface plasmon resonance imaging arrays","volume":"10","author":"Ouellet","year":"2010","journal-title":"Lab Chip"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"16028","DOI":"10.1073\/pnas.1005264107","article-title":"Label-free imaging, detection, and mass measurement of single viruses by surface plasmon resonance","volume":"107","author":"Wang","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1039\/b800606g","article-title":"Microfluidic device for immunoassays based on surface plasmon resonance imaging","volume":"8","author":"Luo","year":"2008","journal-title":"Lab Chip"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"3882","DOI":"10.1039\/c2lc40455a","article-title":"Real-time full-spectral imaging and affinity measurements from 50 microfluidic channels using nanohole surface plasmon resonance","volume":"12","author":"Lee","year":"2012","journal-title":"Lab Chip"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1007\/s00216-004-2636-8","article-title":"Surface plasmon resonance imaging","volume":"379","author":"Steiner","year":"2004","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ab.2006.10.040","article-title":"Higher-throughput, label-free, real-time molecular interaction analysis","volume":"361","author":"Rich","year":"2007","journal-title":"Anal. Biochem."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"2104","DOI":"10.1016\/j.bios.2004.09.025","article-title":"A new surface plasmon resonance sensor for high-throughput screening applications","volume":"20","author":"Piliarik","year":"2005","journal-title":"Biosens. Bioelectron."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.ab.2005.12.024","article-title":"An innovative spectroelectrochemical reflection cell for rapid protein electrochemistry and ultraviolet\/visible\/infrared spectroscopy","volume":"351","author":"Bernad","year":"2006","journal-title":"Anal. Biochem."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1021\/ac034964v","article-title":"Microspotting streptavidin and double-stranded DNA arrays on gold for high-throughput studies of protein-DNA interactions by surface plasmon resonance microscopy","volume":"76","author":"Zareie","year":"2004","journal-title":"Anal. Chem."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1039\/b919584j","article-title":"Optofluidic platforms based on surface-enhanced raman scattering","volume":"135","author":"Lim","year":"2010","journal-title":"Analyst"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1039\/b414457k","article-title":"Highly sensitive signal detection of duplex dye-labelled DNA oligonucleotides in a pdms microfluidic chip: Confocal surface-enhanced raman spectroscopic study","volume":"5","author":"Park","year":"2005","journal-title":"Lab Chip"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.1021\/ac0615246","article-title":"A reproducible surface-enhanced raman spectroscopy approach. Online sers measurements in a segmented microfluidic system","volume":"79","author":"Strehle","year":"2007","journal-title":"Anal. Chem."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"2518","DOI":"10.1039\/c1lc20277d","article-title":"In situ dynamic measurements of the enhanced sers signal using an optoelectrofluidic sers platform","volume":"11","author":"Hwang","year":"2011","journal-title":"Lab Chip"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1039\/C0LC00117A","article-title":"Optoelectrofluidic platforms for chemistry and biology","volume":"11","author":"Hwang","year":"2011","journal-title":"Lab Chip"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"014103","DOI":"10.1063\/1.3086600","article-title":"Enhanced discrimination of normal oocytes using optically induced pulling-up dielectrophoretic force","volume":"3","author":"Hwang","year":"2009","journal-title":"Biomicrofluidics"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"5865","DOI":"10.1021\/ac901047v","article-title":"Dynamic light-activated control of local chemical concentration in a fluid","volume":"81","author":"Hwang","year":"2009","journal-title":"Anal. Chem."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1039\/c3lc41249k","article-title":"In situ synthesis of silver nanoparticle decorated vertical nanowalls in a microfluidic device for ultrasensitive in-channel sers sensing","volume":"13","author":"Parisi","year":"2013","journal-title":"Lab Chip"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1126\/science.1159499","article-title":"Measurement of the distribution of site enhancements in surface-enhanced raman scattering","volume":"321","author":"Fang","year":"2008","journal-title":"Science"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"3720","DOI":"10.1039\/c2lc40353f","article-title":"SERS-based immunoassay using a gold array-embedded gradient microfluidic chip","volume":"12","author":"Lee","year":"2012","journal-title":"Lab Chip"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"5160","DOI":"10.1039\/c2lc40890b","article-title":"Simultaneous detection of duplex DNA oligonucleotides using a sers-based micro-network gradient chip","volume":"12","author":"Choi","year":"2012","journal-title":"Lab Chip"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1039\/c003477k","article-title":"Lensfree microscopy on a cellphone","volume":"10","author":"Tseng","year":"2010","journal-title":"Lab Chip"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1038\/srep01717","article-title":"Increased space-bandwidth product in pixel super-resolved lensfree on-chip microscopy","volume":"3","author":"Greenbaum","year":"2013","journal-title":"Sci. Rep."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"4015","DOI":"10.1039\/c3lc50589h","article-title":"Smart-phone based computational microscopy using multi-frame contact imaging on a fiber-optic array","volume":"13","author":"Navruz","year":"2013","journal-title":"Lab Chip"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"2950","DOI":"10.1039\/c3lc50306b","article-title":"Rapid electrochemical detection on a mobile phone","volume":"13","author":"Lillehoj","year":"2013","journal-title":"Lab Chip"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"2124","DOI":"10.1039\/c3lc40991k","article-title":"Label-free biodetection using a smartphone","volume":"13","author":"Gallegos","year":"2013","journal-title":"Lab Chip"}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/14\/9\/17008\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:15:52Z","timestamp":1760217352000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/14\/9\/17008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,12]]},"references-count":89,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2014,9]]}},"alternative-id":["s140917008"],"URL":"https:\/\/doi.org\/10.3390\/s140917008","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,12]]}}}