{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T02:08:06Z","timestamp":1776132486869,"version":"3.50.1"},"reference-count":34,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2014,11,18]],"date-time":"2014-11-18T00:00:00Z","timestamp":1416268800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No.30600143"],"award-info":[{"award-number":["No.30600143"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["No. Y13H180019"],"award-info":[{"award-number":["No. Y13H180019"]}]},{"name":"Zhejiang province key science and technology innovation team project","award":["No. 2011R50029"],"award-info":[{"award-number":["No. 2011R50029"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A colloidal gold immunochromatographic assay (GICA) was developed for rapid detection of chloramphenicol (CAP) residues in aquatic products. A nitrocellulose (NC) membrane was used as the carrier, and the polyclonal CAP antibody was used as the marker protein. The average diameter of as-prepared colloidal gold nanoparticles (AuNPs) was about 20 nm. The optimal pH value of colloidal gold solutions and the amount  of the antibody of CAP were 8.0 and 7.2 \u03bcg\/mL, respectively. The CAP antibody was immobilized onto the conjugate pad after purification. The CAP conjugate and goat  anti-rabbit IgG (secondary antibody) were coated onto the NC membrane. Next, the  non-specific sites were blocked with 1% bovine serum albumin. The minimum detectable concentration of CAP in standard solution is 0.5 ng\/mL, with good reproducibility. For the real samples from crucian carps injected with a single-dose of CAP in the dorsal muscles, the minimum detectable concentration of CAP residues was 0.5 \u00b5g\/kg. The chromatographic analysis time was less than 10 min, and the strip had a long storage lifetime of more than 90 days at different temperatures. The strips provide a means for rapid detection of CAP residues in aquatic products.<\/jats:p>","DOI":"10.3390\/s141121872","type":"journal-article","created":{"date-parts":[[2014,11,18]],"date-time":"2014-11-18T11:39:36Z","timestamp":1416310776000},"page":"21872-21888","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Rapid Detection of Chloramphenicol Residues in Aquatic Products Using Colloidal Gold Immunochromatographic Assay"],"prefix":"10.3390","volume":"14","author":[{"given":"Chennan","family":"Zhou","sequence":"first","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueyin","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinxin","family":"Huang","sequence":"additional","affiliation":[{"name":"Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xishan","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Cai","sequence":"additional","affiliation":[{"name":"Yangtze Delta Region Institute of Tsinghua University, Jiaxing 310013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Songming","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2014,11,18]]},"reference":[{"key":"ref_1","unstructured":"Hall, S.J., Delaporte, A., Phillips, M.J., Beveridge, M., and O'Keefe, M. 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