{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T21:47:16Z","timestamp":1768340836939,"version":"3.49.0"},"reference-count":19,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2014,11,27]],"date-time":"2014-11-27T00:00:00Z","timestamp":1417046400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Micro-Raman Spectroscopy is an efficient method for analyzing biological specimens due to its sensitivity to subtle chemical and structural changes. The aim of this study was to use micro-Raman spectroscopy to analyze chemical and structural changes in periodontal ligament after orthodontic force application and in gingival crevicular fluid in presence of periodontal disease. The biopsy of periodontal ligament samples of premolars extracted for orthodontic reasons and the gingival crevicular fluid samples collected by using absorbent paper cones; were analyzed by micro-Raman spectroscopy. Changes  of the secondary protein structure related to different times of orthodontic force application were reported; whereas an increase of carotene was revealed in patients affected by periodontal inflammation.<\/jats:p>","DOI":"10.3390\/s141222552","type":"journal-article","created":{"date-parts":[[2014,11,27]],"date-time":"2014-11-27T10:41:20Z","timestamp":1417084880000},"page":"22552-22563","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Micro-Raman Spectroscopy for Monitoring Changes  in Periodontal Ligaments and Gingival Crevicular Fluid"],"prefix":"10.3390","volume":"14","author":[{"given":"Carlo","family":"Camerlingo","sequence":"first","affiliation":[{"name":"CNR-SPIN, Istituto Superconduttori, Materiali Innovativi e Dispositivi, via Campi Flegrei 34, Pozzuoli 80078, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabrizia","family":"D'Apuzzo","sequence":"additional","affiliation":[{"name":"Dip. Multidisciplinare di Specialit\u00e0 Medico-Chirurgiche e Odontoiatriche, Seconda Universit\u00e0 di Napoli, via L. De Crecchio 6, Napoli 80138, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6671-2380","authenticated-orcid":false,"given":"Vincenzo","family":"Grassia","sequence":"additional","affiliation":[{"name":"Dip. Multidisciplinare di Specialit\u00e0 Medico-Chirurgiche e Odontoiatriche, Seconda Universit\u00e0 di Napoli, via L. De Crecchio 6, Napoli 80138, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Letizia","family":"Perillo","sequence":"additional","affiliation":[{"name":"Dip. Multidisciplinare di Specialit\u00e0 Medico-Chirurgiche e Odontoiatriche, Seconda Universit\u00e0 di Napoli, via L. De Crecchio 6, Napoli 80138, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Lepore","sequence":"additional","affiliation":[{"name":"Dip. di Medicina Sperimentale, Seconda Universit\u00e0 di Napoli, S. Maria di Costantinopoli 16,  Napoli 80138, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2014,11,27]]},"reference":[{"key":"ref_1","first-page":"545","article-title":"An immunohistochemical, histological, and electron-microscopic study of the human periodontal ligament during orthodontic treatment","volume":"21","author":"Anastasi","year":"2008","journal-title":"Int. J. Mol. Med."},{"key":"ref_2","first-page":"1","article-title":"Cytokines and VEGF Induction in Orthodontic Movement in Animal Models","volume":"2012","author":"Feola","year":"2012","journal-title":"J. Biomed. 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