{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T16:37:33Z","timestamp":1778863053079,"version":"3.51.4"},"reference-count":47,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2015,1,26]],"date-time":"2015-01-26T00:00:00Z","timestamp":1422230400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC: 81371898"],"award-info":[{"award-number":["NSFC: 81371898"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this study, we describe a novel universal and highly sensitive strategy for the electrochemiluminescent (ECL) detection of sequence specific DNA at the aM level based on Nt.BbvCI (a nicking endonuclease)-assisted target recycling amplification (TRA), rolling circle amplification (RCA) and hemin\/G-quadruplex. The target DNAs can hybridize with self-assembled capture probes and assistant probes to form \u201cY\u201d junction structures on the electrode surface, thus triggering the execution of a TRA reaction with the aid of Nt.BbvCI. Then, the RCA reaction and the addition of hemin result in the production of numerous hemin\/G-quadruplex, which consume the dissolved oxygen in the detection buffer and result in a significant ECL quenching effect toward the O2\/S2O82\u2212 system. The proposed strategy combines the amplification ability of TRA, RCA and the inherent high sensitivity of the ECL technique, thus enabling low aM (3.8 aM) detection for sequence-specific DNA and a wide linear range from 10.0 aM to 1.0 pM. At the same time, this novel strategy shows high selectivity against single-base mismatch sequences, which makes our novel universal and highly sensitive method a powerful addition to specific DNA sequence detection.<\/jats:p>","DOI":"10.3390\/s150202629","type":"journal-article","created":{"date-parts":[[2015,1,26]],"date-time":"2015-01-26T13:26:37Z","timestamp":1422278797000},"page":"2629-2643","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["A Novel Ultrasensitive ECL Sensor for DNA Detection Based on Nicking Endonuclease-Assisted Target Recycling Amplification, Rolling Circle Amplification and Hemin\/G-Quadruplex"],"prefix":"10.3390","volume":"15","author":[{"given":"Fukang","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Xinqiao Hospital, Third Military Medical University,  Chongqing 400037, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guimin","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Xinqiao Hospital, Third Military Medical University,  Chongqing 400037, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyun","family":"Pu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Xinqiao Hospital, Third Military Medical University,  Chongqing 400037, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juanchun","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Xinqiao Hospital, Third Military Medical University,  Chongqing 400037, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Xinqiao Hospital, Third Military Medical University,  Chongqing 400037, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guohui","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Xinqiao Hospital, Third Military Medical University,  Chongqing 400037, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2015,1,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"E354","DOI":"10.1111\/1469-0691.12231","article-title":"Prospective study of a panfungal pcr assay followed by sequencing, for the detection of fungal DNA in normally sterile specimens in a clinical setting: A complementary tool in the diagnosis of invasive fungal disease?","volume":"19","author":"Babouee","year":"2013","journal-title":"Clin. 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