{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T20:39:35Z","timestamp":1761597575543,"version":"build-2065373602"},"reference-count":21,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2015,1,27]],"date-time":"2015-01-27T00:00:00Z","timestamp":1422316800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>This paper presents a microfluidics-based approach capable of continuously characterizing instantaneous Young\u2019s modulus (Einstantaneous) and specific membrane capacitance (Cspecific membrane) of suspended single cells. In this method, cells were aspirated through a constriction channel while the cellular entry process into the constriction channel was recorded using a high speed camera and the impedance profiles at two frequencies  (1 kHz and 100 kHz) were simultaneously measured by a lock-in amplifier. Numerical simulations were conducted to model cellular entry process into the constriction channel, focusing on two key parameters: instantaneous aspiration length (Linstantaneous) and transitional aspiration length (Ltransitional), which was further translated to Einstantaneous. An equivalent distribution circuit model for a cell travelling in the constriction channel was used to determine Cspecific membrane. A non-small-cell lung cancer cell line 95C (n = 354) was used to evaluate this technique, producing Einstantaneous of 2.96 \u00b1 0.40 kPa and Cspecific membrane of 1.59 \u00b1 0.28 \u03bcF\/cm2. As a platform for continuous and simultaneous characterization of cellular Einstantaneous and Cspecific membrane, this approach can facilitate a more comprehensive understanding of cellular biophysical properties.<\/jats:p>","DOI":"10.3390\/s150202763","type":"journal-article","created":{"date-parts":[[2015,1,27]],"date-time":"2015-01-27T09:57:28Z","timestamp":1422352648000},"page":"2763-2773","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Simultaneous Characterization of Instantaneous Young\u2019s Modulus and Specific Membrane Capacitance of Single  Cells Using a Microfluidic System"],"prefix":"10.3390","volume":"15","author":[{"given":"Yang","family":"Zhao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deyong","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yana","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoting","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wentao","family":"Yue","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, University of Alberta, Edmonton, AB T6G 2G8, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junbo","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4612-3279","authenticated-orcid":false,"given":"Jian","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2015,1,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ethier, C.R., and Simmons, C.A. 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