{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T22:48:16Z","timestamp":1774392496304,"version":"3.50.1"},"reference-count":27,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2015,3,4]],"date-time":"2015-03-04T00:00:00Z","timestamp":1425427200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Murine monoclonal antibodies to target Salmonella typhimurium flagellin and lipopolysaccharide (LPS) were prepared and characterized. For the immunological detection of S. typhimurium, different pairs of monoclonal antibodies (MAbs) were tested in a sandwich enzyme linked immunosorbent assay (ELISA) format. After comparison, a sandwich ELISA and immunochromatographic strip based on LPS MAbs was established to detect S. typhimurium. The determination limits of the immunochromatographic strip in phosphate-buffered saline (PBS) containing 0.1% Tween 20 (PBST) and pure milk sample were found to be 1.25 \u00d7 105 colony-forming units (cfu)\/mL and 1.25 \u00d7 106 cfu\/mL  S. typhimurium, respectively. Results can be obtained with the naked eye in 10 min.  Cross-reactivity was observed with Salmonella paratyphi B, but not S. paratyphi A or Salmonella enteritidis. The LPS MAbs based immunochromatographic strip is rapid and convenient to detect S. typhimurium in milk samples.<\/jats:p>","DOI":"10.3390\/s150305281","type":"journal-article","created":{"date-parts":[[2015,3,4]],"date-time":"2015-03-04T10:19:49Z","timestamp":1425464389000},"page":"5281-5292","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":78,"title":["A Highly Sensitive ELISA and Immunochromatographic Strip for the Detection of Salmonella typhimurium in Milk Samples"],"prefix":"10.3390","volume":"15","author":[{"given":"Wenbin","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Lab of Food Science and Technology, School of Food Science and Technology,  Jiangnan University, Wuxi 214122, China"}]},{"given":"Liqiang","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Lab of Food Science and Technology, School of Food Science and Technology,  Jiangnan University, Wuxi 214122, China"}]},{"given":"Shanshan","family":"Song","sequence":"additional","affiliation":[{"name":"State Key Lab of Food Science and Technology, School of Food Science and Technology,  Jiangnan University, Wuxi 214122, China"}]},{"given":"Lijuan","family":"Tang","sequence":"additional","affiliation":[{"name":"State Key Lab of Food Science and Technology, School of Food Science and Technology,  Jiangnan University, Wuxi 214122, China"}]},{"given":"Hua","family":"Kuang","sequence":"additional","affiliation":[{"name":"State Key Lab of Food Science and Technology, School of Food Science and Technology,  Jiangnan University, Wuxi 214122, China"}]},{"given":"Chuanlai","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Lab of Food Science and Technology, School of Food Science and Technology,  Jiangnan University, Wuxi 214122, China"}]}],"member":"1968","published-online":{"date-parts":[[2015,3,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.meegid.2014.10.003","article-title":"Tracing the sources of human salmonellosis: A multi-model comparison of phenotyping and genotyping methods","volume":"28","author":"Smid","year":"2014","journal-title":"Infect. Genet. Evol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.foodcont.2014.06.036","article-title":"Survival of Salmonella enteric in skim milk powder with different water activity and water mobility","volume":"47","author":"Lian","year":"2015","journal-title":"Food Control"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1111\/jam.12207","article-title":"Production and evaluation of the utility of novel phage display-derived peptide ligands to Salmonella spp. for magnetic separation","volume":"115","author":"Morton","year":"2013","journal-title":"J. Appl. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1128\/IAI.72.2.795-809.2004","article-title":"Role of the Salmonella Pathogenicity Island 1 Effector Proteins SipA, SopB, SopE, and SopE2 in Salmonella enterica Subspecies 1 Serovar Typhimurium Colitis in Streptomycin-Pretreated Mice","volume":"72","author":"Hapfelmeier","year":"2004","journal-title":"Infect. Immun."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1186\/1471-2180-8-84","article-title":"Multi-locus variable-number tandem repeat analysis for outbreak studies of Salmonella enterica serotype Enteritidis","volume":"8","author":"Malorny","year":"2008","journal-title":"BMC Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4187","DOI":"10.1039\/c2ay25997d","article-title":"A new competitive fluorescence immunoassay for detection of Listeria monocytogenes","volume":"4","author":"Beauchamp","year":"2012","journal-title":"Anal. Methods"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1502","DOI":"10.1111\/j.1365-2621.2011.02646.x","article-title":"Multiplex PCR for the simultaneous detection of Salmonella SPP. and Salmonella Enteritidis in food","volume":"46","author":"Silva","year":"2011","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.bios.2011.09.031","article-title":"Highly sensitive detection of Salmonella typhi using surface aminated polycarbonate membrane enhanced-ELISA","volume":"31","author":"Jain","year":"2012","journal-title":"Biosens. Bioelectron."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2828","DOI":"10.1016\/j.bios.2010.10.001","article-title":"Immunochromatographic strip test for detection of genus Cronobacter","volume":"26","author":"Fukal","year":"2011","journal-title":"Biosens. Bioelectron."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1598","DOI":"10.3390\/ijerph10041598","article-title":"Monoclonal Antibody-Based Sandwich ELISA for the Detection of Staphylococcal Enterotoxin A","volume":"10","author":"Kuang","year":"2013","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.ijfoodmicro.2010.12.030","article-title":"Development of monoclonal antibody based sandwich ELISA for the rapid detection of pathogenic Vibrio parahaemolyticus in seafood","volume":"145","author":"Kumar","year":"2011","journal-title":"Int. J. Food Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1128\/jcm.22.6.1040-1044.1985","article-title":"Method for the isolation of highly purified Salmonella flagellins","volume":"22","author":"Ibrahim","year":"1985","journal-title":"J. Clin. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0378-1135(00)00291-1","article-title":"Analysis of expression of flagella by Salmonella enterica serotype Typhimurium by monoclonal antibodies recognising both phase specific and common epitopes","volume":"78","author":"Sojka","year":"2001","journal-title":"Vet. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1128\/iai.55.1.211-216.1987","article-title":"A murine monoclonal antibody specific for the outer core oligosaccharide of Salmonella lipopolysaccharide","volume":"55","author":"Tsang","year":"1987","journal-title":"Infect. Immun."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1093\/infdis\/161.1.59","article-title":"Production of specific monoclonal antibodies to Salmonella typhi flagellin and possible application to immunodiagnosis of typhoid fever","volume":"161","author":"Sadallah","year":"1990","journal-title":"J. Infect. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1111\/j.1365-2672.1992.tb01814.x","article-title":"Sandwich capture ELISA by a murine monoclonal antibody against a genus-specific LPS epitope for the detection of different common serotypes of salmonellas","volume":"72","author":"Choi","year":"1992","journal-title":"J. Appl. Bacteriol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/09540105.2012.716398","article-title":"Development of a monoclonal antibody-based sandwich ELISA for the detection of ovalbumin in foods","volume":"25","author":"Peng","year":"2014","journal-title":"Food Agric. Immunol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1080\/09540105.2012.716026","article-title":"Development of a monoclonal antibody-based ELISA to detect Escherichia coli O157: H7","volume":"24","author":"Feng","year":"2013","journal-title":"Food Agric. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4214","DOI":"10.3390\/s130404214","article-title":"Rapid and highly sensitive detection of lead ions in drinking water based on a strip immunosensor","volume":"13","author":"Kuang","year":"2013","journal-title":"Sensors"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.1016\/j.foodchem.2013.04.098","article-title":"A label-free electrochemical impedance immunosensor based on AuNPs\/PAMAM-MWCNT-Chi nanocomposite modified glassy carbon electrode for detection of Salmonella typhimurium in milk","volume":"141","author":"Dong","year":"2013","journal-title":"Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1128\/jcm.22.3.347-351.1985","article-title":"Production of potent Salmonella H antisera by immunization with polymeric flagellins","volume":"22","author":"Ibrahim","year":"1985","journal-title":"J. Clin. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1610","DOI":"10.1128\/jb.177.6.1610-1613.1995","article-title":"Identification of the domain which determines the g, m serotype of the flagellin of Salmonella enteritidis","volume":"177","author":"Zwaagstra","year":"1995","journal-title":"J. Bacteriol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/0022-1759(90)90445-2","article-title":"Efficient production of mouse and rat monoclonal antibodies against the O antigens of Salmonella serogroup C1 using LPS-coated bacteria as immunogen","volume":"129","author":"Luk","year":"1990","journal-title":"J. Immunol. Methods"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/S1369-5274(02)00302-8","article-title":"Regulation of flagellar assembly","volume":"5","author":"Aldridge","year":"2002","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0895-3988(08)60019-7","article-title":"Enrichment-ELISA for detection of Salmonella typhi from food and water samples","volume":"21","author":"Kumar","year":"2008","journal-title":"Biomed. Environ. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1016\/j.bios.2011.10.031","article-title":"Development of a one-step immunochromatographic strip test using gold nanoparticles for the rapid detection of Salmonella typhi in human serum","volume":"31","author":"Preechakasedkit","year":"2012","journal-title":"Biosens. Bioelectron."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"8858","DOI":"10.1039\/C4AY01373E","article-title":"Electrochemical immunosensors, genosensors and phagosensors for Salmonella detection","volume":"6","author":"Alegret","year":"2014","journal-title":"Anal. 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