{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T03:33:43Z","timestamp":1773804823353,"version":"3.50.1"},"reference-count":32,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2015,4,15]],"date-time":"2015-04-15T00:00:00Z","timestamp":1429056000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Whole-cell Systemic Evolution of Ligands by Exponential enrichment (SELEX) is the process by which aptamers specific to target cells are developed. Aptamers selected by whole-cell SELEX have high affinity and specificity for bacterial surface molecules and live bacterial targets. To identify DNA aptamers specific to Staphylococcus aureus, we applied our rapid whole-cell SELEX method to a single-stranded ssDNA library.  To improve the specificity and selectivity of the aptamers, we designed, selected, and developed two categories of aptamers that were selected by two kinds of whole-cell SELEX, by mixing and combining FACS analysis and a counter-SELEX process. Using this approach, we have developed a biosensor system that employs a high affinity aptamer for detection of target bacteria. FAM-labeled aptamer sequences with high binding to  S. aureus, as determined by fluorescence spectroscopic analysis, were identified, and aptamer A14, selected by the basic whole-cell SELEX using a once-off FACS analysis, and which had a high binding affinity and specificity, was chosen. The binding assay was evaluated using FACS analysis. Our study demonstrated the development of a set of whole-cell SELEX derived aptamers specific to S. aureus; this approach can be used in the identification of other bacteria.<\/jats:p>","DOI":"10.3390\/s150408884","type":"journal-article","created":{"date-parts":[[2015,4,15]],"date-time":"2015-04-15T10:51:24Z","timestamp":1429095084000},"page":"8884-8897","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":76,"title":["Comparison of Whole-Cell SELEX Methods for the Identification of Staphylococcus Aureus-Specific DNA Aptamers"],"prefix":"10.3390","volume":"15","author":[{"given":"Jihea","family":"Moon","sequence":"first","affiliation":[{"name":"National Academy of Agricultural Science, 310 Nongsaengmyeng-ro, Wansan-gu, Jeonju 560500, Korea"}]},{"given":"Giyoung","family":"Kim","sequence":"additional","affiliation":[{"name":"National Academy of Agricultural Science, 310 Nongsaengmyeng-ro, Wansan-gu, Jeonju 560500, Korea"}]},{"given":"Saet","family":"Park","sequence":"additional","affiliation":[{"name":"National Academy of Agricultural Science, 310 Nongsaengmyeng-ro, Wansan-gu, Jeonju 560500, Korea"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2501-4367","authenticated-orcid":false,"given":"Jongguk","family":"Lim","sequence":"additional","affiliation":[{"name":"National Academy of Agricultural Science, 310 Nongsaengmyeng-ro, Wansan-gu, Jeonju 560500, Korea"}]},{"given":"Changyeun","family":"Mo","sequence":"additional","affiliation":[{"name":"National Academy of Agricultural Science, 310 Nongsaengmyeng-ro, Wansan-gu, Jeonju 560500, Korea"}]}],"member":"1968","published-online":{"date-parts":[[2015,4,15]]},"reference":[{"key":"ref_1","first-page":"63","article-title":"Staphylococcus aureus and food poisoning","volume":"2","author":"Loir","year":"2003","journal-title":"Gen. 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