{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T19:06:22Z","timestamp":1772564782209,"version":"3.50.1"},"reference-count":44,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2015,4,29]],"date-time":"2015-04-29T00:00:00Z","timestamp":1430265600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>An indirect competitive enzyme-linked immunosorbent assay (icELISA) and  an immunochromatographic strip assay using a highly specific monoclonal antibody, were developed to detect methyltestosterone (MT) residues in animal feed. The optimized icELISA had a half-inhibition concentration value of 0.26 ng\/mL and a limit of detection value of 0.045 ng\/mL. There was no cross-reactivity with eight analogues, revealing  high specificity for MT. Based on icELISA results, the recovery rate of MT in animal  feed was 82.4%\u2013100.6%. The results were in accordance with those obtained by gas  chromatography-mass spectrometry. The developed immunochromatographic strip assay, as the first report for MT detection, had a visual cut-off value of 1 ng\/mL in PBS, 2.5 ng\/g in fish feed, and 2.5 ng\/g in pig feed. Therefore, these immunoassays are useful and fast tools for MT residue detection in animal feed.<\/jats:p>","DOI":"10.3390\/s150510059","type":"journal-article","created":{"date-parts":[[2015,4,29]],"date-time":"2015-04-29T11:02:35Z","timestamp":1430305355000},"page":"10059-10073","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Sensitive, Fast, and Specific Immunoassays for Methyltestosterone Detection"],"prefix":"10.3390","volume":"15","author":[{"given":"Na","family":"Kong","sequence":"first","affiliation":[{"name":"State Key Lab of Food Science and Technology, School of Food Science and Technology,  Jiangnan University, Wuxi 214122, China"}]},{"given":"Shanshan","family":"Song","sequence":"additional","affiliation":[{"name":"State Key Lab of Food Science and Technology, School of Food Science and Technology,  Jiangnan University, Wuxi 214122, China"}]},{"given":"Juan","family":"Peng","sequence":"additional","affiliation":[{"name":"State Key Lab of Food Science and Technology, School of Food Science and Technology,  Jiangnan University, Wuxi 214122, China"}]},{"given":"Liqiang","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Lab of Food Science and Technology, School of Food Science and Technology,  Jiangnan University, Wuxi 214122, China"}]},{"given":"Hua","family":"Kuang","sequence":"additional","affiliation":[{"name":"State Key Lab of Food Science and Technology, School of Food Science and Technology,  Jiangnan University, Wuxi 214122, China"}]},{"given":"Chuanlai","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Lab of Food Science and Technology, School of Food Science and Technology,  Jiangnan University, Wuxi 214122, China"}]}],"member":"1968","published-online":{"date-parts":[[2015,4,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.tvjl.2003.10.020","article-title":"Technologies for the control of fat and lean deposition in livestock","volume":"167","author":"Sillence","year":"2004","journal-title":"Vet. 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