{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T03:39:11Z","timestamp":1777347551676,"version":"3.51.4"},"reference-count":37,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2015,5,18]],"date-time":"2015-05-18T00:00:00Z","timestamp":1431907200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Fluorescence spectra and fluorescence lifetime images of talaporfin sodium and sodium-pheophorbide a, which can be regarded as photosensitizers for photodynamic therapy, were measured in normal and cancer cells. The reduction of the fluorescence intensity by photoirradiation was observed for both photosensitizers in both cells, but the quenching rate was much faster in cancer cells than in normal cells. These results are explained in terms of the excessive generation of reactive oxygen species via photoexcitation of these photosensitizers in cancer cells. The fluorescence lifetimes of both photosensitizers in cancer cells are different from those in normal cells, which originates from the different intracellular environments around the photosensitizers between normal and cancer cells.<\/jats:p>","DOI":"10.3390\/s150511417","type":"journal-article","created":{"date-parts":[[2015,5,18]],"date-time":"2015-05-18T10:56:37Z","timestamp":1431946597000},"page":"11417-11430","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Fluorescence Characteristics and Lifetime Images of Photosensitizers of Talaporfin Sodium and Sodium Pheophorbide a in Normal and Cancer Cells"],"prefix":"10.3390","volume":"15","author":[{"given":"Kamlesh","family":"Awasthi","sequence":"first","affiliation":[{"name":"Research Institute for Electronic Science (RIES), Hokkaido University, Sapporo 001-0020, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhito","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Graduate School of Photonic Science, Chitose Institute for Science and Technology, Chitose 066-8655, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazunari","family":"Furuya","sequence":"additional","affiliation":[{"name":"Graduate School of Photonic Science, Chitose Institute for Science and Technology, Chitose 066-8655, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takakazu","family":"Nakabayashi","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-ku, Sendai 980-8578, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liming","family":"Li","sequence":"additional","affiliation":[{"name":"Graduate School of Photonic Science, Chitose Institute for Science and Technology, Chitose 066-8655, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuhiro","family":"Ohta","sequence":"additional","affiliation":[{"name":"Research Institute for Electronic Science (RIES), Hokkaido University, Sapporo 001-0020, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2015,5,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1001\/archderm.1960.01580040026005","article-title":"The Photodynamic Properties of a Particular Hematoporphyrin Derivative","volume":"82","author":"Lipson","year":"1960","journal-title":"Arch. Dermatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1039\/cs9952400019","article-title":"Photosensitizers of the Porphyrin and Phthalocyanine Series for Photodynamic Therapy","volume":"24","author":"Bonnett","year":"1995","journal-title":"Chem. Soc. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1038\/nrc1071","article-title":"Photodynamic Therapy for Cancer","volume":"3","author":"Dolmans","year":"2003","journal-title":"Nat. Rev. Cancer"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1039\/B915149B","article-title":"The Role of Porphyrin Chemistry in Tumor Imaging and Photodynamic Therapy","volume":"40","author":"Ethirajan","year":"2011","journal-title":"Chem. Soc. Rev."},{"key":"ref_5","first-page":"763","article-title":"Anticancer Effects of Novel Photodynamic Therapy with Glycoconjugated Chlorin for Gastric and Colon Cancer","volume":"31","author":"Tanaka","year":"2011","journal-title":"Anticancer Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/bjp.2008.98","article-title":"Photodynamic Therapy: Novel Third-Generation Photosensitizers One Step Closer?","volume":"154","author":"Josefsen","year":"2008","journal-title":"Br. J. Pharmacol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1111\/j.1751-1097.1987.tb04849.x","article-title":"Localization of Mono-L-Aspartyl Chlorin e6 (NPe6) in Mouse Tissues","volume":"46","author":"Aizawa","year":"1987","journal-title":"Photochem. Photobiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/1011-1344(93)80006-U","article-title":"Photosensitizing Properties of Mono-L-Aspartyl Chlorin e6 (NPe6): A Candidate Sensitizer for the Photodynamic Therapy of Tumors","volume":"17","author":"Spikes","year":"1993","journal-title":"J. Photochem. Photobiol. B"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1517\/14656560903463893","article-title":"Talaporfin Sodium","volume":"11","author":"Wang","year":"2010","journal-title":"Expert Opin. Pharmacother."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S1011-1344(02)00244-0","article-title":"Phase-Resolved Fluorescence Study of Mono-L-Aspartyl Chlorin E6","volume":"67","author":"Li","year":"2002","journal-title":"J. Photochem. Photobiol. B"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1080\/15421400902945592","article-title":"The Photocytotoxicity of Talaporfin Used in Photodynamic Therapy","volume":"505","author":"Li","year":"2009","journal-title":"Mol. Cryst. Liq. Cryst."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1080\/15421400902942151","article-title":"Optical Properties of Talaporfin-Serum Albumin Complex","volume":"505","author":"Shirogane","year":"2009","journal-title":"Mol. Cryst. Liq. Cryst."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/S0169-5002(03)00242-3","article-title":"Phase II Clinical Study of Photodynamic Therapy Using Mono-L-Aspartyl Chlorin e6 and Diode Laser for Early Superficial Squamous Cell Carcinoma of the Lung","volume":"42","author":"Kato","year":"2003","journal-title":"Lung Cancer"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.lungcan.2007.06.026","article-title":"Photodynamic Therapy for Lung Cancers Based on Novel Photodynamic Diagnosis Using Talaporfin Sodium (NPe6) and Autofluorescence Bronchoscopy","volume":"58","author":"Usuda","year":"2007","journal-title":"Lung Cancer"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1186\/1748-717X-7-113","article-title":"Phase I Study of Photodynamic Therapy Using Talaporfin Sodium and Diode Laser for Local Failure after Chemoradiotherapy for Esophageal Cancer","volume":"7","author":"Yano","year":"2012","journal-title":"Radiat. Oncol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1002\/lsm.1900090308","article-title":"New Method of Photosensitizer Accumulation for Photodynamic Therapy in an Experimental Liver Tumor","volume":"9","author":"Nishikawa","year":"1989","journal-title":"Laser. Surg. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1089\/pho.2008.2319","article-title":"Bactericidal Effect of Photodynamic Therapy Using Na-Pheophorbide a: Evaluation of Adequate Light Source","volume":"27","author":"Yamamoto","year":"2009","journal-title":"Photomed. Laser Surg."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1089\/pho.2010.2803","article-title":"Therapeutic Effect of Photodynamic Therapy Using Na-Pheophorbide a on Osteomyelitis Models in Rats","volume":"29","author":"Goto","year":"2011","journal-title":"Photomed. Laser Surg."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"180","DOI":"10.2184\/lsj.35.3_180","article-title":"A Comparative Study between Pulsed and Continuous Wave Irradiation for Talaporfin Sodium Mediated Photosensitization in Solution, in-vitro and in-vivo","volume":"35","author":"Ohmori","year":"2007","journal-title":"Rev. Laser Eng."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1111\/php.12027","article-title":"Effects of the Oxygenation Level on Formation of Different Reactive Oxygen Species during Photodynamic Therapy","volume":"89","author":"Price","year":"2013","journal-title":"Photochem. Photobiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/1011-1344(90)80009-M","article-title":"Photophysical Studies of Pheophorbide a and Pheophytin a. Phosphorescence and Photosensitized Singlet Oxygen Luminescence","volume":"5","author":"Krasnovsky","year":"1990","journal-title":"J. Photochem. Photobiol. B"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1117\/1.1352750","article-title":"Mechanism of Photodynamic Activity of Pheophorbides","volume":"6","author":"Tanielian","year":"2001","journal-title":"J. Biomed. Opt."},{"key":"ref_23","first-page":"1772","article-title":"Pheophorbide A-Mediated Photodynamic Therapy Induces Apoptotic Cell Death in Murine Oral Squamous Cell Carcinoma in vitro and in vivo","volume":"27","author":"Ahn","year":"2012","journal-title":"Oncol. Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1080\/15421401003613634","article-title":"Discrimination of Normal and Cancer Cells from Autofluorescence Spectra by Laser Excitation","volume":"519","author":"Shirogane","year":"2010","journal-title":"Mol. Cryst. Liq. Cryst."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"10385","DOI":"10.1021\/jp2058904","article-title":"Intracellular pH Sensing Using Autofluorescence Lifetime Microscopy","volume":"115","author":"Ogikubo","year":"2011","journal-title":"J. Phys. Chem. B"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1063\/1.1638874","article-title":"Time-Resolved Measurement System of Electrofluorescence Spectra","volume":"75","author":"Tsushima","year":"2004","journal-title":"Rev. Sci. Istrum."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"521","DOI":"10.4236\/jct.2012.35067","article-title":"Rapid and Simple Analysis of N-Aspartylchlorin e6 (Talaporfin) Using Fluorescence Microtiterplate and Its Application for Determination in Cells, Tissues and Blood","volume":"3","author":"Yokoyama","year":"2012","journal-title":"J. Cancer Ther."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.freeradbiomed.2011.03.042","article-title":"Cell Death via Mitochondrial Apoptotic Pathway due to Activation of Bax by Lysosomal Photodamage","volume":"51","author":"Liu","year":"2011","journal-title":"Free Radic. Biol. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1248\/bpb.b12-00567","article-title":"Photodynamic Therapy in Combination with Talaporfin Sodium Induces Mitochondrial Apoptotic Cell Death Accompanied with Necrosis in Glioma Cells","volume":"36","author":"Miki","year":"2013","journal-title":"Biol. Pharm. Bull."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"11315","DOI":"10.1523\/JNEUROSCI.3590-07.2007","article-title":"Rapid Activation of Antioxidant Defenses by Nerve Growth Factor Suppresses Reactive Oxygen Species during Neuronal Apoptosis: Evidence for a Role in Cytochrome c Redistribution","volume":"17","author":"Kirkland","year":"2007","journal-title":"J. Neurosci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.ultsonch.2010.05.002","article-title":"Detection and Analysis of Reactive Oxygen Species (ROS) Generated by Nano-Sized TiO2 Powder under Ultrasonic Irradiation and Application in Sonocatalytic Degradation of Organic Dyes","volume":"18","author":"Wang","year":"2011","journal-title":"Ultrason. Sonochem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2641","DOI":"10.1021\/cr900343z","article-title":"Fluorescence Lifetime Measurements and Biological Imaging","volume":"12","author":"Berezin","year":"2010","journal-title":"Chem. Rev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"275","DOI":"10.2116\/analsci.31.275","article-title":"Sensing of Intracellular Environments by Fluorescence Lifetime Imaging of Exogenous Fluorophores","volume":"31","author":"Nakabayashi","year":"2015","journal-title":"Anal. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1038\/nature11706","article-title":"How Cancer Metabolism is Tuned for Proliferation and Vulnerable to Disruption","volume":"491","author":"Schulze","year":"2012","journal-title":"Nature"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nrc3110","article-title":"Dysregulated pH: A Perfect Storm for Cancer Progression","volume":"11","author":"Webb","year":"2011","journal-title":"Nat. Rev. Cancer"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"34211","DOI":"10.1074\/jbc.M109.041152","article-title":"Microenvironmental pH is a Key Factor for Exosome Traffic in Tumor Cells","volume":"284","author":"Parolini","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e103126","DOI":"10.1371\/journal.pone.0103126","article-title":"Preclinical Validation of Talaporfin Sodium-Mediated Photodynamic Therapy for Esophageal Squamous Cell Carcinoma","volume":"9","author":"Ohashi","year":"2014","journal-title":"PLoS ONE"}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/15\/5\/11417\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T20:46:28Z","timestamp":1760215588000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/15\/5\/11417"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,18]]},"references-count":37,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2015,5]]}},"alternative-id":["s150511417"],"URL":"https:\/\/doi.org\/10.3390\/s150511417","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,18]]}}}