{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T14:16:02Z","timestamp":1773843362657,"version":"3.50.1"},"reference-count":39,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2015,5,28]],"date-time":"2015-05-28T00:00:00Z","timestamp":1432771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A fluorescence-based fiber optic toxicity biosensor based on genetically modified Escherichia coli (E. coli) with green fluorescent protein (GFP) was developed for the evaluation of the toxicity of several hazardous heavy metal ions. The toxic metals include Cu(II), Cd(II), Pb(II), Zn(II), Cr(VI), Co(II), Ni(II), Ag(I) and Fe(III). The optimum fluorescence excitation and emission wavelengths of the optical biosensor were 400 \u00b1 2 nm and 485 \u00b1 2 nm, respectively. Based on the toxicity observed under optimal conditions, the detection limits of Cu(II), Cd(II), Pb(II), Zn(II), Cr(VI), Co(II), Ni(II), Ag(I) and Fe(III) that can be detected using the toxicity biosensor were at 0.04, 0.32, 0.46, 2.80, 100, 250, 400, 720 and 2600 \u03bcg\/L, respectively. The repeatability and reproducibility of the proposed biosensor were 3.5%\u20134.8% RSD (relative standard deviation) and 3.6%\u20135.1% RSD (n = 8), respectively. The biosensor response was stable for at least five weeks, and demonstrated higher sensitivity towards metal toxicity evaluation when compared to a conventional Microtox assay.<\/jats:p>","DOI":"10.3390\/s150612668","type":"journal-article","created":{"date-parts":[[2015,5,28]],"date-time":"2015-05-28T11:01:49Z","timestamp":1432810909000},"page":"12668-12681","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["An Optical Biosensor from Green Fluorescent Escherichia coli for the Evaluation of Single and Combined Heavy Metal Toxicities"],"prefix":"10.3390","volume":"15","author":[{"given":"Dedi","family":"Futra","sequence":"first","affiliation":[{"name":"School of Chemical Science and Food Technolgoy, Faculty of Science,  Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D.E., Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lee","family":"Heng","sequence":"additional","affiliation":[{"name":"School of Chemical Science and Food Technolgoy, Faculty of Science,  Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D.E., Malaysia"},{"name":"Southeast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), LESTARI,  Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D.E., Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asmat","family":"Ahmad","sequence":"additional","affiliation":[{"name":"School of Chemical Science and Food Technolgoy, Faculty of Science,  Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D.E., Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salmijah","family":"Surif","sequence":"additional","affiliation":[{"name":"School of Chemical Science and Food Technolgoy, Faculty of Science,  Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D.E., Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tan","family":"Ling","sequence":"additional","affiliation":[{"name":"Southeast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), LESTARI,  Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D.E., Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2015,5,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1351\/pac200274050793","article-title":"Heavy metals\u2014A meaningless term; chemistry and human health division clinical chemistry section commission on toxicology","volume":"74","author":"Duffus","year":"2002","journal-title":"Pure Appl. 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