{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T19:04:08Z","timestamp":1778785448210,"version":"3.51.4"},"reference-count":32,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2015,10,12]],"date-time":"2015-10-12T00:00:00Z","timestamp":1444608000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A miniature quartz crystal microbalance (mQCM) was integrated with a polydimethylsiloxane (PDMS) microfluidic device for on-chip determination of amyloid polypeptide\u2013A\u03b242. The integration techniques included photolithography and plasma coupling. A\u03b242 antibody was immobilized on the mQCM surface using a cross-linker method, and the resonance frequency of mQCM shifted negatively due to antibody-antigen binding. A linear range from 0.1 \u00b5M to 3.2 \u00b5M was achieved. By using matrix elimination buffer, i.e., matrix phosphate buffer containing 500 \u00b5g\/mL dextran and 0.5% Tween 20, A\u03b242 could be successfully detected in the presence of 75% human serum. Additionally, high temperature treatments at 150 \u00b0C provided a valid method to recover mQCM, and PDMS-mQCM microfluidic device could be reused to some extent. Since the detectable A\u03b242 concentration could be as low as 0.1 \u00b5M, which is close to cut-off value for Alzheimer patients, the PDMS-mQCM device could be applied in early Alzheimer\u2019s disease diagnosis.<\/jats:p>","DOI":"10.3390\/s151025746","type":"journal-article","created":{"date-parts":[[2015,10,14]],"date-time":"2015-10-14T02:36:30Z","timestamp":1444790190000},"page":"25746-25760","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Integration of a Miniature Quartz Crystal Microbalance with a Microfluidic Chip for Amyloid Beta-A\u03b242 Quantitation"],"prefix":"10.3390","volume":"15","author":[{"given":"Wenyan","family":"Tao","sequence":"first","affiliation":[{"name":"Shenzhen Key Laboratory of Special Functional Materials & Shenzhen Engineering Laboratory for Advance Technology of Ceramics, College of Materials Science and Engineering,  Shenzhen University, Shenzhen 518060, China"},{"name":"College of Electronic Science and Technology, Shenzhen University, Shenzhen 518060, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingji","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hairui","family":"Wang","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Special Functional Materials & Shenzhen Engineering Laboratory for Advance Technology of Ceramics, College of Materials Science and Engineering,  Shenzhen University, Shenzhen 518060, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanming","family":"Ke","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Special Functional Materials & Shenzhen Engineering Laboratory for Advance Technology of Ceramics, College of Materials Science and Engineering,  Shenzhen University, Shenzhen 518060, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Lin","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Special Functional Materials & Shenzhen Engineering Laboratory for Advance Technology of Ceramics, College of Materials Science and Engineering,  Shenzhen University, Shenzhen 518060, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xierong","family":"Zeng","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Special Functional Materials & Shenzhen Engineering Laboratory for Advance Technology of Ceramics, College of Materials Science and Engineering,  Shenzhen University, Shenzhen 518060, China"},{"name":"College of Electronic Science and Technology, Shenzhen University, Shenzhen 518060, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2015,10,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"513","DOI":"10.2174\/0929866054395905","article-title":"The role of Abeta peptides in Alzhermer\u2019s disease","volume":"12","author":"Tickler","year":"2005","journal-title":"Protein Pept. 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