{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T10:26:14Z","timestamp":1775298374836,"version":"3.50.1"},"reference-count":64,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2016,1,11]],"date-time":"2016-01-11T00:00:00Z","timestamp":1452470400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A fluorescence chemosensor, 2-hydroxy-1-naphthaldehyde azine (HNA) was designed and synthesized for sequential detection of Cu2+ and biothiols. It was found that HNA can specifically bind to Cu2+ with 1:1 stoichiometry, accompanied with a dramatic fluorescence quenching and a remarkable bathochromic-shift of the absorbance peak in HEPES buffer. The generated HNA-Cu2+ ensemble displayed a \u201cturn-on\u201d fluorescent response specific for biothiols (Hcy, Cys and GSH) based on the displacement approach, giving a remarkable recovery of fluorescence and UV-Vis spectra. The detection limits of HNA-Cu2+ to Hcy, Cys and GSH were estimated to be 1.5 \u03bcM, 1.0 \u03bcM and 0.8 \u03bcM, respectively, suggesting that HNA-Cu2+ is sensitive enough for the determination of thiols in biological systems. The biocompatibility of HNA towards A549 human lung carcinoma cell, was evaluated by an MTT assay. The capability of HNA-Cu2+ to detect biothiols in live A549 cells was then demonstrated by a microscopy fluorescence imaging assay.<\/jats:p>","DOI":"10.3390\/s16010079","type":"journal-article","created":{"date-parts":[[2016,1,11]],"date-time":"2016-01-11T10:09:38Z","timestamp":1452506978000},"page":"79","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Synthesis and Application of an Aldazine-Based Fluorescence Chemosensor for the Sequential Detection of Cu2+ and Biological Thiols in Aqueous Solution and Living Cells"],"prefix":"10.3390","volume":"16","author":[{"given":"Hongmin","family":"Jia","sequence":"first","affiliation":[{"name":"Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan 114051, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan 114051, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingtao","family":"Meng","sequence":"additional","affiliation":[{"name":"Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan 114051, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangjie","family":"He","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine, Xinxiang Medical University, Xinxiang, He\u2018nan 453003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan 114051, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhizhi","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan 114051, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0943-824X","authenticated-orcid":false,"given":"Run","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiqiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan 114051, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,1,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1016\/j.snb.2013.06.080","article-title":"Bioactive paper platform for colorimetric phenols detection","volume":"186","author":"Liu","year":"2013","journal-title":"Sens. Actuators B Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3094","DOI":"10.1016\/j.ccr.2006.08.017","article-title":"Anion recognition and sensing in organic and aqueous media using luminescent and colorimetric sensors","volume":"250","author":"Gunnlaugsson","year":"2006","journal-title":"Coord. Chem. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"4511","DOI":"10.1039\/c2cs00004k","article-title":"Fluorescent chemodosimeters using \u201cmild\u201d chemical events for the detection of small anions and cations in biological and environmental media","volume":"41","author":"Du","year":"2012","journal-title":"Chem. Soc. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1039\/C4CS00263F","article-title":"Design strategies for lab-on-a-molecule probes and orthogonal sensin","volume":"44","author":"Chen","year":"2015","journal-title":"Chem. Soc. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"9233","DOI":"10.1016\/j.tet.2011.09.003","article-title":"Diastereo- and enantioselective aldol reaction of granatanone (pseudopelletierine)","volume":"67","author":"Kaur","year":"2011","journal-title":"Tetrahedron"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.snb.2013.03.062","article-title":"Comparative study of colorimetric sensors based on newly synthesized Schiff bases","volume":"182","author":"Gupta","year":"2013","journal-title":"Sens. Actuators B Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3443","DOI":"10.1021\/cr068000q","article-title":"Tools and Tactics for the Optical Detection of Mercuric Ion","volume":"108","author":"Nolan","year":"2008","journal-title":"Chem. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0039-9140(03)00118-8","article-title":"Preparation of ethambutol\u2013copper (II) complex and fabrication of PVC based membrane potentiometric sensor for copper","volume":"60","author":"Gupta","year":"2003","journal-title":"Talanta"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4564","DOI":"10.1021\/cr400546e","article-title":"Fluorescent Sensors for Measuring Metal Ions in Living Systems","volume":"114","author":"Carter","year":"2014","journal-title":"Chem. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1016\/j.saa.2014.10.061","article-title":"Substitutional group dependent colori\/fluorimetric sensing of Mn2+, Fe3+ and Zn2+ ions by simple Schiff base chemosensor","volume":"136","author":"Hariharan","year":"2015","journal-title":"Spectrochim. Acta A"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3440","DOI":"10.1016\/S0021-9258(19)83641-4","article-title":"A new generation of Ca2+ indicators with greatly improved fluorescence properties","volume":"260","author":"Grynkiewicz","year":"1985","journal-title":"J. Biol. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6132","DOI":"10.1002\/anie.200901266","article-title":"A Color-Tunable Europium Complex Emitting Three Primary Colors and White Light","volume":"48","author":"He","year":"2009","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1039\/b818611a","article-title":"A red-shift colorimetric and fluorescent sensor for Cu2+ in aqueous solution: Unsymmetrical 4,5-diaminonaphthalimide with N-H deprotonation induced by metal ions","volume":"7","author":"Huang","year":"2009","journal-title":"Org. Biomol. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1646","DOI":"10.1021\/ic402767j","article-title":"Boron-Dipyrromethene Based Reversible and Reusable Selective Chemosensor for Fluoride Detection","volume":"53","author":"Madhu","year":"2014","journal-title":"Inorg. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4242","DOI":"10.1021\/ol401932p","article-title":"A N-(2-Aminophenyl)-5-(dimethylamino)-1-naphthalenesulfonic Amide (Ds-DAB) Based Fluorescent Chemosensor for Peroxynitrite","volume":"15","author":"Lin","year":"2013","journal-title":"Org. Lett."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/j.bios.2013.06.004","article-title":"A FRET ratiometric fluorescence sensing system for mercury detection and intracellular colorimetric imaging in live Hela cells","volume":"49","author":"Hu","year":"2013","journal-title":"Biosens. Bioelectron."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"6032","DOI":"10.1039\/c3cs60055f","article-title":"Thiol-addition reactions and their applications in thiol recognition","volume":"42","author":"Yin","year":"2013","journal-title":"Chem. Soc. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2333","DOI":"10.1016\/j.ccr.2012.03.009","article-title":"Probing oxidative stress: Small molecule fluorescent sensors of metal ions, reactive oxygen species, and thiols","volume":"256","author":"Hyman","year":"2012","journal-title":"Coord. Chem. Rev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2120","DOI":"10.1039\/b925092a","article-title":"Fluorescent and colorimetric probes for detection of thiols","volume":"39","author":"Chen","year":"2010","journal-title":"Chem. Soc. Rev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"6967","DOI":"10.1039\/c4cc00243a","article-title":"Selective homocysteine turn-on fluorescent probes and their bioimaging applications","volume":"50","author":"Lee","year":"2014","journal-title":"Chem. Commun."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/S0968-0004(02)00003-8","article-title":"Structure, mechanism and regulation of peroxiredoxins","volume":"28","author":"Wood","year":"2003","journal-title":"Trends Biochem. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.ab.2006.01.047","article-title":"Fluorescence-based detection of thiols in vitro and in vivo using dithiol probes","volume":"352","author":"Pullela","year":"2006","journal-title":"Anal. Biochem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1016\/S0891-5849(00)00215-X","article-title":"Regulation of redox glutathione levels and gene transcription in lung inflammation: The rapeutic approaches","volume":"28","author":"Rahman","year":"2000","journal-title":"Free Radic. Biol. Med."},{"key":"ref_24","unstructured":"Voet, D., and Voet, J.G. (1995). Biochemistry, John Wiley & Sons. [2nd ed.]."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.bios.2015.01.019","article-title":"A readily available colorimetric and near-infrared fluorescent turn-on probe for rapid and selective detection of cysteine in living cells","volume":"68","author":"Xue","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2029","DOI":"10.1039\/C4CC08156K","article-title":"A highly selective and sensitive fluorescent thiol probe through dual-reactive and dual-quenching groups","volume":"51","author":"Zhang","year":"2015","journal-title":"Chem. Commun."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1753-4887.1996.tb03770.x","article-title":"Biological chemistry of thiols in the vasculature and in vascular-related disease","volume":"54","author":"Stamler","year":"1996","journal-title":"Nutr. Rev."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.dyepig.2013.09.021","article-title":"A colorimetric and fluorescent probe for thiols based on 1, 8-naphthalimide and its application for bioimaging","volume":"100","author":"Shi","year":"2014","journal-title":"Dyes Pigm."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"8219","DOI":"10.1039\/C4CC03527E","article-title":"Differences in heterocycle basicity distinguish homocysteine from cysteine using aldehyde-bearing fluorophores","volume":"50","author":"Barve","year":"2014","journal-title":"Chem. Commun."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1093\/jn\/134.3.489","article-title":"Glutathione metabolism and its implications for health","volume":"134","author":"Wu","year":"2004","journal-title":"J. Nutr."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"5972","DOI":"10.1021\/ac010541m","article-title":"Lead Phthalocyanine as a Selective Carrier for Preparation of a Cysteine-Selective Electrode","volume":"73","author":"Shahrokhian","year":"2001","journal-title":"Anal. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1039\/C0JM02497J","article-title":"Colorimetric and near-infrared fluorescence turn-on molecular probe for direct and highly selective detection of cysteine in human plasma","volume":"21","author":"Hao","year":"2011","journal-title":"J. Mater. Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"6078","DOI":"10.1016\/j.biomaterials.2014.04.035","article-title":"Live cells imaging using a turn-on FRET-based BODIPY probe for biothiols","volume":"35","author":"Su","year":"2014","journal-title":"Biomaterials"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4402","DOI":"10.1021\/am500102s","article-title":"Flavone-Based ESIPT Ratiometric Chemodosimeter for Detection of Cysteine in Living Cells","volume":"6","author":"Liu","year":"2014","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1039\/b925366a","article-title":"Evaluation of different analytical strategies for the quantification of sulfur-containing biomolecules by HPLC-ICP-MS: Application to the characterisation of 34S-labelled yeast","volume":"25","author":"Sanz","year":"2010","journal-title":"J. Anal. Atom. Spectrom."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2757","DOI":"10.1039\/C3CS60444F","article-title":"Photodissociation mass spectrometry: New tools for characterization of biological molecules","volume":"43","author":"Brodbelt","year":"2014","journal-title":"Chem. Soc. Rev."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1568","DOI":"10.1039\/C0CP00980F","article-title":"Sensitive and selective electrochemical sensing of L-cysteine based on a caterpillar-like manganese dioxide\u2013carbon nanocomposite","volume":"13","author":"Xiao","year":"2011","journal-title":"Phys. Chem. Chem. Phys."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.aca.2011.02.051","article-title":"Development of a heterobimetallic Ru(II)\u2013Cu(II) complex for highly selective and sensitive luminescence sensing of sulfide anions","volume":"691","author":"Zhang","year":"2011","journal-title":"Anal. Chim. Acta"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.dyepig.2012.07.013","article-title":"A fluorescent sensor bearing nitroolefin moiety for the detection of thiols and its biological imaging","volume":"96","author":"Wang","year":"2013","journal-title":"Dyes Pigm."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"9986","DOI":"10.1021\/ja809345d","article-title":"Thiol-Selective Fluorogenic Probes for Labeling and Release","volume":"131","author":"Hong","year":"2009","journal-title":"J. Am. Chem. Soc."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1039\/C0OB00957A","article-title":"Fluorescent chemodosimeter for Cys\/Hcy with a large absorption shift and imaging in living cells","volume":"9","author":"Hu","year":"2011","journal-title":"Org. Biomol. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"7721","DOI":"10.1039\/c3ob41414k","article-title":"Lighting up cysteine and homocysteine in sequence based on the kinetic difference of the cyclization\/addition reaction","volume":"11","author":"Guo","year":"2013","journal-title":"Org. Biomol. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"11666","DOI":"10.1021\/ja072572q","article-title":"A Rhodamine-Based Fluorescent Probe Containing a Se-N Bond for Detecting Thiols and Its Application in Living Cells","volume":"129","author":"Tang","year":"2007","journal-title":"J. Am. Chem. Soc."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1039\/c3cc48128j","article-title":"A near-infrared colorimetric fluorescent chemodosimeter for the detection of glutathione in living cells","volume":"50","author":"Li","year":"2014","journal-title":"Chem. Commun."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"6604","DOI":"10.1039\/c3ob41284a","article-title":"Designing a thiol specific fluorescent probe for possible use as a reagent for intracellular detection and estimation in blood serum: Kinetic analysis to probe the role of intramolecular hydrogen bonding","volume":"11","author":"Das","year":"2013","journal-title":"Org. Biomol. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.aca.2014.09.004","article-title":"A sensitive and selective detection method for thiol compounds using novel fluorescence probe","volume":"850","author":"Zheng","year":"2014","journal-title":"Anal. Chim. Acta"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1892","DOI":"10.1039\/c1an15014f","article-title":"Indicator approach to develop a chemosensor for the colorimetric sensing of thiol-containing water and its application for the thiol detection in plasma","volume":"136","author":"Huo","year":"2011","journal-title":"Analyst"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1016\/j.inoche.2009.09.035","article-title":"Highly selective colorimetric detection of cysteine and homocysteine in water through a direct displacement approach","volume":"12","author":"Zhang","year":"2009","journal-title":"Inorg. Chem. Commun."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"5493","DOI":"10.1039\/c3ay40977e","article-title":"1,4-Dihydroxyanthraquinone\u2013Cu2+ ensemble probe for selective detection of sulfide anion in aqueous solution","volume":"5","author":"Wang","year":"2013","journal-title":"Anal. Methods"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3197","DOI":"10.1039\/C4CC00091A","article-title":"Organelle-selective fluorescent Cu2+ ion probes: Revealing the endoplasmic reticulum as a reservoir for Cu-overloading","volume":"50","author":"Lee","year":"2014","journal-title":"Chem. Comm."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"6207","DOI":"10.1039\/C4CC00521J","article-title":"A coumarin-based fluorescent probe for recognition of Cu2+ and fast detection of histidine in hard-to-transfect cells by a sensing ensemble approach","volume":"50","author":"You","year":"2014","journal-title":"Chem. Commun."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.bios.2015.06.077","article-title":"A highly selective and sensitive ON\u2013OFF\u2013ON fluorescence chemosensor for cysteine detection in endoplasmic reticulum","volume":"74","author":"Meng","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"9762","DOI":"10.1016\/j.tet.2010.09.043","article-title":"Ratiometric fluorescence chemosensors for copper(II) and mercury(II) based on FRET systems","volume":"66","author":"He","year":"2010","journal-title":"Tetrahedron"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1039\/AN9871201183","article-title":"Extraction-spectrophotometric determination of hydrazine with 2-hydroxy-1-naphthaldehyde","volume":"112","author":"Campillos","year":"1987","journal-title":"Aanlyst"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"5056","DOI":"10.1021\/ol2018856","article-title":"Coumarin-Cu(II) Ensemble-Based Cyanide Sensing Chemodosimeter","volume":"13","author":"Jung","year":"2011","journal-title":"Org. Lett."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"7390","DOI":"10.1039\/b916476f","article-title":"Sulfide-selective chemosignaling by a Cu2+ complex of dipicolylamine appended fluorescein","volume":"47","author":"Choi","year":"2009","journal-title":"Chem. Comm."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bios.2013.06.005","article-title":"Highly sensitive and selective phosphorescent chemosensors for hypochlorous acid based on ruthenium(II) complexes","volume":"50","author":"Zhang","year":"2013","journal-title":"Biosens. Bioelectron."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"7695","DOI":"10.1039\/c1cc12018b","article-title":"Highly selective, sensitive and quantitative detection of Hg2+ in aqueous medium under broad pH range","volume":"27","author":"Koteeswari","year":"2011","journal-title":"Chem. Comm."},{"key":"ref_59","unstructured":"Dean, J.A. (1999). Lang\u00e8s Handbook of Chemistry, McGraw-Hill. [15th ed.]."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1016\/j.biomaterials.2011.10.040","article-title":"A cysteine-selective fluorescent probe for the cellular detection of cysteine","volume":"33","author":"Jung","year":"2012","journal-title":"Biomaterials"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"2034","DOI":"10.1016\/j.tet.2014.01.060","article-title":"A fluorescence turn-on probe for the detection of thiol-containing amino acids in aqueous solution and bioimaging in cells","volume":"70","author":"Son","year":"2014","journal-title":"Tetrahedron"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.1126\/science.1523409","article-title":"Oxidized redox state of glutathione in the endoplasmic reticulum","volume":"257","author":"Hwang","year":"1992","journal-title":"Science"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1021\/ja408792k","article-title":"Highly Selective Off\u2013On Fluorescent Probe for Imaging Thioredoxin Reductase in Living Cells","volume":"136","author":"Zhang","year":"2014","journal-title":"J. Am. Chem. Soc."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2411","DOI":"10.1002\/asia.201500690","article-title":"Reversible and Selective Fluorescence Detection of Histidine Using a Naphthalimide-Based Chemosensing Ensemble","volume":"10","author":"Meng","year":"2015","journal-title":"Chem. Asian J."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/16\/1\/79\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T19:17:31Z","timestamp":1760210251000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/16\/1\/79"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,11]]},"references-count":64,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,1]]}},"alternative-id":["s16010079"],"URL":"https:\/\/doi.org\/10.3390\/s16010079","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,11]]}}}