{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T04:16:20Z","timestamp":1772252180499,"version":"3.50.1"},"reference-count":45,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2016,2,23]],"date-time":"2016-02-23T00:00:00Z","timestamp":1456185600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>For the first time a molecularly imprinted polymer (MIP) with direct electron transfer (DET) and bioelectrocatalytic activity of the target protein is presented. Thin films of MIPs for the recognition of a hexameric tyrosine-coordinated heme protein (HTHP) have been prepared by electropolymerization of scopoletin after oriented assembly of HTHP on a self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA) on gold electrodes. Cavities which should resemble the shape and size of HTHP were formed by template removal. Rebinding of the target protein sums up the recognition by non-covalent interactions between the protein and the MIP with the electrostatic attraction of the protein by the SAM. HTHP bound to the MIP exhibits quasi-reversible DET which is reflected by a pair of well pronounced redox peaks in the cyclic voltammograms (CVs) with a formal potential of \u2212184.4 \u00b1 13.7 mV vs. Ag\/AgCl (1 M KCl) at pH 8.0 and it was able to catalyze the cathodic reduction of peroxide. At saturation the MIP films show a 12-fold higher electroactive surface concentration of HTHP than the non-imprinted polymer (NIP).<\/jats:p>","DOI":"10.3390\/s16030272","type":"journal-article","created":{"date-parts":[[2016,2,23]],"date-time":"2016-02-23T10:57:06Z","timestamp":1456225026000},"page":"272","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Molecularly Imprinted Electropolymer for a Hexameric Heme Protein with Direct Electron Transfer and Peroxide Electrocatalysis"],"prefix":"10.3390","volume":"16","author":[{"given":"Lei","family":"Peng","sequence":"first","affiliation":[{"name":"Institute of Biochemistry and Biology, Potsdam University, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aysu","family":"Yarman","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Biology, Potsdam University, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4528-1478","authenticated-orcid":false,"given":"Katharina","family":"Jetzschmann","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Biology, Potsdam University, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae-Hun","family":"Jeoung","sequence":"additional","affiliation":[{"name":"Institute of Biology, Structural Biology\/Biochemistry, Humboldt-Universit\u00e4t zu Berlin, Unter den Linden 6, 10099 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Schad","sequence":"additional","affiliation":[{"name":"Institute of Biology, Structural Biology\/Biochemistry, Humboldt-Universit\u00e4t zu Berlin, Unter den Linden 6, 10099 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Holger","family":"Dobbek","sequence":"additional","affiliation":[{"name":"Institute of Biology, Structural Biology\/Biochemistry, Humboldt-Universit\u00e4t zu Berlin, Unter den Linden 6, 10099 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7088-8928","authenticated-orcid":false,"given":"Ulla","family":"Wollenberger","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Biology, Potsdam University, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frieder","family":"Scheller","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Biology, Potsdam University, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam, Germany"},{"name":"Fraunhofer Institute for Cell Therapy and Immunology IZI-BB, Am M\u00fchlenberg 13, 14476 Potsdam, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,2,23]]},"reference":[{"key":"ref_1","first-page":"341","article-title":"Use of polymers with enzyme-analogous structures for resolution of racemates","volume":"11","author":"Wulff","year":"1972","journal-title":"Angew. 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