{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T18:46:34Z","timestamp":1778870794406,"version":"3.51.4"},"reference-count":28,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2016,3,15]],"date-time":"2016-03-15T00:00:00Z","timestamp":1458000000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this study, we have investigated the contribution of bovine serum albumin (BSA) to the durability of the electrochemically synthesized poly(3,4-ethylenedioxythiophene) (PEDOT) film on a platinum (Pt) electrode. The electrode was capable to effectively adsorb the nano Au particles (AuNPs) to form a uniform layout, which was then able to immobilize the horseradish peroxidase (HRP) to construct a functional HRP\/AuNPs\/PEDOT(BSA)\/Pt biosensor. Cyclic voltammetry was employed to evaluate the performance of the biosensor through the measurement of hydrogen peroxide. Our results revealed a satisfied linear correlation between the cathodic current and the concentration of H2O2. Furthermore, the addition of oxidized form of nicotinamide adenine dinucleotide, or NAD+, as the electron transfer mediator in the detection solution could dramatically enhance the sensitivity of detection by about 35.5%. The main advantages of the current biosensor are its durability, sensitivity, reliability, and biocompatibility.<\/jats:p>","DOI":"10.3390\/s16030374","type":"journal-article","created":{"date-parts":[[2016,3,15]],"date-time":"2016-03-15T11:10:18Z","timestamp":1458040218000},"page":"374","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["A Novel Conductive Poly(3,4-ethylenedioxythiophene)-BSA Film for the Construction of a Durable HRP Biosensor Modified with NanoAu Particles"],"prefix":"10.3390","volume":"16","author":[{"given":"Fangcheng","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Chemical and Biochemical Engineering, Xiamen University, No. 422, Siming South Road, Xiamen 361005, Fujian, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuaibin","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biochemical Engineering, Xiamen University, No. 422, Siming South Road, Xiamen 361005, Fujian, China"},{"name":"Department of Chemical and Materials Engineering, Tunghai University, No.1727, Sec.4, Taiwan Boulevard, Xitun District, Taichung 40704, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yesong","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Chemical and Materials Engineering, Tunghai University, No.1727, Sec.4, Taiwan Boulevard, Xitun District, Taichung 40704, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,3,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.synthmet.2004.12.011","article-title":"Electronic, chemical and structural change induced by organic solvents in tosylate-doped poly(3,4-ethylenedioxythiophene) (PEDOT-OTs)","volume":"149","author":"Kim","year":"2005","journal-title":"Synth. 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