{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T16:10:04Z","timestamp":1761581404146,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2016,3,15]],"date-time":"2016-03-15T00:00:00Z","timestamp":1458000000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In 2013 alone, the death rate among the 9.0 million people infected with Mycobacterium tuberculosis (TB) worldwide was around 14%, which is unacceptably high. An empiric treatment of patients infected with TB or drug-resistant Mycobacterium tuberculosis (MDR-TB) strain can also result in the spread of MDR-TB. The diagnostic tools which are rapid, reliable, and have simple experimental protocols can significantly help in decreasing the prevalence rate of MDR-TB strain. We report the evaluation of the 9G technology based 9G DNAChips that allow accurate detection and discrimination of TB and MDR-TB-RIF. One hundred and thirteen known cultured samples were used to evaluate the ability of 9G DNAChip in the detection and discrimination of TB and MDR-TB-RIF strains. Hybridization of immobilized probes with the PCR products of TB and MDR-TB-RIF strains allow their detection and discrimination. The accuracy of 9G DNAChip was determined by comparing its results with sequencing analysis and drug susceptibility testing. Sequencing analysis showed 100% agreement with the results of 9G DNAChip. The 9G DNAChip showed very high sensitivity (95.4%) and specificity (100%).<\/jats:p>","DOI":"10.3390\/s16030376","type":"journal-article","created":{"date-parts":[[2016,3,15]],"date-time":"2016-03-15T11:10:18Z","timestamp":1458040218000},"page":"376","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Accurate Detection of Rifampicin-Resistant Mycobacterium Tuberculosis Strains"],"prefix":"10.3390","volume":"16","author":[{"given":"Keum-Soo","family":"Song","sequence":"first","affiliation":[{"name":"Biometrix Technology, Inc. 202 BioVenture Plaza, Chuncheon 200-161, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satish","family":"Nimse","sequence":"additional","affiliation":[{"name":"Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0128-2789","authenticated-orcid":false,"given":"Hee","family":"Kim","sequence":"additional","affiliation":[{"name":"Korea Mycobacterium Resource Center (KMRC), The Korean Institute of Tuberculosis, Cheongju-si 28158, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeongseong","family":"Yang","sequence":"additional","affiliation":[{"name":"Korea Mycobacterium Resource Center (KMRC), The Korean Institute of Tuberculosis, Cheongju-si 28158, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taisun","family":"Kim","sequence":"additional","affiliation":[{"name":"Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,3,15]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (WHO) (2014). 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