{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T11:45:36Z","timestamp":1768909536195,"version":"3.49.0"},"reference-count":46,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2016,4,20]],"date-time":"2016-04-20T00:00:00Z","timestamp":1461110400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The research on intelligent bioelectrocatalysis based on stimuli-responsive materials or interfaces is of great significance for biosensors and other bioelectronic devices. In the present work, lectin protein concanavalin A (Con A) and glycoenzyme glucose oxidase (GOD) were assembled into {Con A\/GOD}n layer-by-layer (LbL) films by taking advantage of the biospecific lectin-glycoenzyme affinity between them. These film electrodes possess stimuli-responsive properties toward electroactive probes such as ferrocenedicarboxylic acid (Fc(COOH)2) by modulating the surrounding pH. The CV peak currents of Fc(COOH)2 were quite large at pH 4.0 but significantly suppressed at pH 8.0, demonstrating reversible stimuli-responsive on-off behavior. The mechanism of stimuli-responsive property of the films was explored by comparative experiments and attributed to the different electrostatic interaction between the films and the probes at different pH. This stimuli-responsive films could be used to realize active\/inactive electrocatalytic oxidation of glucose by GOD in the films and mediated by Fc(COOH)2 in solution, which may establish a foundation for fabricating novel stimuli-responsive electrochemical biosensors based on bioelectrocatalysis with immobilized enzymes.<\/jats:p>","DOI":"10.3390\/s16040563","type":"journal-article","created":{"date-parts":[[2016,4,20]],"date-time":"2016-04-20T11:00:40Z","timestamp":1461150040000},"page":"563","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A Stimuli-Responsive Biosensor of Glucose on Layer-by-Layer Films Assembled through Specific Lectin-Glycoenzyme Recognition"],"prefix":"10.3390","volume":"16","author":[{"given":"Huiqin","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Ningxia  Medical University, Yinchuan 750004, China"}]},{"given":"Qianqian","family":"Gan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Ningxia  Medical University, Yinchuan 750004, China"}]},{"given":"Juan","family":"Peng","sequence":"additional","affiliation":[{"name":"State Key Laboratory Cultivation Base of Natural Gas Conversion, Ningxia University, Yinchuan 750021, China"},{"name":"School of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8719-8934","authenticated-orcid":false,"given":"Shan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Ningxia  Medical University, Yinchuan 750004, China"}]},{"given":"Meilin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Ningxia  Medical University, Yinchuan 750004, China"}]},{"given":"Keren","family":"Shi","sequence":"additional","affiliation":[{"name":"State Key Laboratory Cultivation Base of Natural Gas Conversion, Ningxia University, Yinchuan 750021, China"}]}],"member":"1968","published-online":{"date-parts":[[2016,4,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2512","DOI":"10.1039\/b714635n","article-title":"Stimuli-responsive surfaces for bio-applications","volume":"37","author":"Mendes","year":"2008","journal-title":"Chem. 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