{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T09:46:25Z","timestamp":1775295985475,"version":"3.50.1"},"reference-count":38,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2016,5,9]],"date-time":"2016-05-09T00:00:00Z","timestamp":1462752000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In the present study, we fabricated a hemoglobin\/gold nanoparticle (Hb\/GNP) heterolayer immobilized on the Au micro-gap to confirm H2O2 detection with a signal-enhancement effect. The hemoglobin which contained the heme group catalyzed the reduction of H2O2. To facilitate the electron transfer between hemoglobin and Au micro-gap electrode, a gold nanoparticle was introduced. The Au micro-gap electrode that has gap size of 5 \u00b5m was fabricated by conventional photolithographic technique to locate working and counter electrodes oppositely in a single chip for the signal sensitivity and reliability. The hemoglobin was self-assembled onto the Au surface via chemical linker 6-mercaptohexanoic acid (6-MHA). Then, the gold nanoparticles were adsorbed onto hemoglobin\/6-MHA heterolayers by the layer-by-layer (LbL) method. The fabrication of the Hb\/GNP heterolayer was confirmed by atomic force microscopy (AFM) and surface-enhanced Raman spectroscopy (SERS). The redox property and H2O2 detection of Hb\/GNP on the micro-gap electrode was investigated by a cyclic voltammetry (CV) experiment. Taken together, the present results show that the electrochemical signal-enhancement effect of a hemoglobin\/nanoparticle heterolayer was well confirmed on the micro-scale electrode for biosensor applications.<\/jats:p>","DOI":"10.3390\/s16050660","type":"journal-article","created":{"date-parts":[[2016,5,9]],"date-time":"2016-05-09T10:05:24Z","timestamp":1462788324000},"page":"660","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Investigation of Hemoglobin\/Gold Nanoparticle Heterolayer on Micro-Gap for Electrochemical Biosensor Application"],"prefix":"10.3390","volume":"16","author":[{"given":"Taek","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Seoul 121-742, Korea"},{"name":"Research Center for Integrated Biotechnology, Sogang University, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Seoul 121-742, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tae-Hyung","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Integrative Engineering, Chung-Ang University, Heukseok-dong, Dongjak-gu, Seoul 156-756, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinho","family":"Yoon","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Seoul 121-742, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong-Ho","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Hoseo University, 20, Hoseo-ro 79beon-gil, Baebang-Eup, Asan City, Chungnam 336-795, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Seoul 121-742, Korea"},{"name":"Research Center for Integrated Biotechnology, Sogang University, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Seoul 121-742, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeong-Woo","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Seoul 121-742, Korea"},{"name":"Research Center for Integrated Biotechnology, Sogang University, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Seoul 121-742, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,5,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Bartlett, P.N. 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