{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T13:51:37Z","timestamp":1760709097607,"version":"build-2065373602"},"reference-count":31,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2016,6,29]],"date-time":"2016-06-29T00:00:00Z","timestamp":1467158400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Taiwan, Hong Kong and Macau Cooperation Project of Chinese Ministry of Science and Technology","award":["2012DFM30040"],"award-info":[{"award-number":["2012DFM30040"]}]},{"name":"the Science and Technology Development Fund of Macau","award":["047\/2013\/A2"],"award-info":[{"award-number":["047\/2013\/A2"]}]},{"name":"the Major Industry-Academy Cooperation Project of Fujian Province, China","award":["2011Y4007"],"award-info":[{"award-number":["2011Y4007"]}]},{"name":"the Major Project of Fujian Province, China","award":["2014YZ0001"],"award-info":[{"award-number":["2014YZ0001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>With many benefits and applications, immunochromatographic (ICG) assay detection systems have been reported on a great deal. However, the existing research mainly focuses on increasing the dynamic detection range or application fields. Calibration of the detection system, which has a great influence on the detection accuracy, has not been addressed properly. In this context, this work develops a calibration strip for ICG assay photoelectric detection systems. An image of the test strip is captured by an image acquisition device, followed by performing a fuzzy c-means (FCM) clustering algorithm and maximin-distance algorithm for image segmentation. Additionally, experiments are conducted to find the best characteristic quantity. By analyzing the linear coefficient, an average value of hue (H) at 14 min is chosen as the characteristic quantity and the empirical formula between H and optical density (OD) value is established. Therefore, H, saturation (S), and value (V) are calculated by a number of selected OD values. Then, H, S, and V values are transferred to the RGB color space and a high-resolution printer is used to print the strip images on cellulose nitrate membranes. Finally, verification of the printed calibration strips is conducted by analyzing the linear correlation between OD and the spectral reflectance, which shows a good linear correlation (R2 = 98.78%).<\/jats:p>","DOI":"10.3390\/s16071007","type":"journal-article","created":{"date-parts":[[2016,6,29]],"date-time":"2016-06-29T17:03:22Z","timestamp":1467219802000},"page":"1007","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Development of a Calibration Strip for Immunochromatographic Assay Detection Systems"],"prefix":"10.3390","volume":"16","author":[{"given":"Yue-Ming","family":"Gao","sequence":"first","affiliation":[{"name":"College of Physics and Information Engineering, Fuzhou University, Fuzhou 350116, China"},{"name":"Key Lab of Medical Instrumentation &amp; Pharmaceutical Technology of Fujian Province, Fuzhou 350116, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian-Chong","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Physics and Information Engineering, Fuzhou University, Fuzhou 350116, China"},{"name":"Key Lab of Medical Instrumentation &amp; Pharmaceutical Technology of Fujian Province, Fuzhou 350116, China"},{"name":"State Key Laboratory of Analog and Mixed Signal VLSI, University of Macau, Macau 999078, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng-Un","family":"Mak","sequence":"additional","affiliation":[{"name":"Key Lab of Medical Instrumentation &amp; Pharmaceutical Technology of Fujian Province, Fuzhou 350116, China"},{"name":"Department of Electrical and Computer Engineering, Faculty of Science and Technology, University of Macau, Macau 999078, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mang-I.","family":"Vai","sequence":"additional","affiliation":[{"name":"Key Lab of Medical Instrumentation &amp; Pharmaceutical Technology of Fujian Province, Fuzhou 350116, China"},{"name":"State Key Laboratory of Analog and Mixed Signal VLSI, University of Macau, Macau 999078, China"},{"name":"Department of Electrical and Computer Engineering, Faculty of Science and Technology, University of Macau, Macau 999078, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Du","sequence":"additional","affiliation":[{"name":"College of Physics and Information Engineering, Fuzhou University, Fuzhou 350116, China"},{"name":"Key Lab of Medical Instrumentation &amp; Pharmaceutical Technology of Fujian Province, Fuzhou 350116, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sio-Hang","family":"Pun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Analog and Mixed Signal VLSI, University of Macau, Macau 999078, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,6,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"8447","DOI":"10.1021\/jf051681q","article-title":"Production of a monoclonal antibody against ochratoxin A and its application to immunochromatographic assay","volume":"53","author":"Cho","year":"2005","journal-title":"J. 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