{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T04:08:12Z","timestamp":1777435692113,"version":"3.51.4"},"reference-count":45,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2016,7,20]],"date-time":"2016-07-20T00:00:00Z","timestamp":1468972800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this work, we present a simple and rapid method to synthesize red luminescent gold nanoclusters (AuNCs) with high quantum yield (QY, ~16%), excellent photostability and biocompatibility. Next, we fabricated a solid membrane by loading the as-prepared AuNCs in an agar matrix. Different from nanomaterials dispersed in solution, the AuNCs-based solid membrane has distinct advantages including convenience of transportation, while still maintaining strong red luminescence, and relatively long duration storage without aggregation. Taking hydrogen peroxide (H2O2) as a typical example, we then employed the AuNCs as a luminescent probe and investigated their sensing performance, either in solution phase or on a solid substrate. The detection of H2O2 could be achieved in wide concentration ranges over 805 nM\u20131.61 mM and 161 \u03bcM\u201319.32 mM in solution and on a solid membrane, respectively, with limits of detection (LOD) of 80 nM and 20 \u03bcM. Moreover, the AuNCs-based membrane could also be used for visual detection of H2O2 in the range of 0\u20133.22 mM. In view of the convenient synthesis route and attractive luminescent properties, the AuNCs-based membrane presented in this work is quite promising for applications such as optical sensing, fluorescent imaging, and photovoltaics.<\/jats:p>","DOI":"10.3390\/s16071124","type":"journal-article","created":{"date-parts":[[2016,7,20]],"date-time":"2016-07-20T09:45:15Z","timestamp":1469007915000},"page":"1124","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Facile Fabrication of a Gold Nanocluster-Based Membrane for the Detection of Hydrogen Peroxide"],"prefix":"10.3390","volume":"16","author":[{"given":"Pu","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Pharmacy, Chongqing Research Center for Pharmaceutical Engineering, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Wang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Green Synthesis and Applications, College of Chemistry, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yibing","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Key Laboratory of Diagnostic Medicine designated by the Ministry of Education, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,7,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1038\/nnano.2009.193","article-title":"Plasmonic fluorescent quantum dots","volume":"4","author":"Jin","year":"2009","journal-title":"Nat. Nanotechnol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"7006","DOI":"10.1021\/ac201488x","article-title":"Highly sensitive fluorescent analysis of dynamic glycan expression on living cells using glyconanoparticles and functionalized quantum dots","volume":"83","author":"Han","year":"2011","journal-title":"Anal. Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"6726","DOI":"10.1002\/anie.200906623","article-title":"Luminescent carbon nanodots: Emergent nanolights","volume":"49","author":"Baker","year":"2010","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"9297","DOI":"10.1002\/anie.201204381","article-title":"Amphiphilic egg-derived carbon dots: Rapid plasma fabrication, pyrolysis process, and multicolor printing patterns","volume":"51","author":"Wang","year":"2012","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"14192","DOI":"10.1021\/ja2048804","article-title":"One-pot microwave synthesis of water-dispersible, ultraphoto- and pH-stable, and highly fluorescent silicon quantum dots","volume":"133","author":"He","year":"2011","journal-title":"J. Am. Chem. Soc."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1039\/C3NR04835G","article-title":"Protein-directed synthesis of pH-responsive red fluorescent copper nanoclusters and their applications in cellular imaging and catalysis","volume":"6","author":"Wang","year":"2014","journal-title":"Nanoscale"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1039\/b920783j","article-title":"Silver nanoclusters as fluorescent probes for selective and sensitive detection of copper ions","volume":"46","author":"Lan","year":"2010","journal-title":"Chem. Commun."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3395","DOI":"10.1039\/b821518a","article-title":"Oligonucleotide-stabilized Ag nanoclusters as novel fluorescence probes for the highly selective and sensitive detection of the Hg2+ ion","volume":"23","author":"Guo","year":"2009","journal-title":"Chem. Commun."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"5484","DOI":"10.1021\/ac300332t","article-title":"Using gold nanoclusters as selective luminescent probes for phosphate-containing metabolites","volume":"84","author":"Li","year":"2012","journal-title":"Anal. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1021\/ac503636j","article-title":"Fluorescent gold nanoclusters: Recent advances in sensing and imaging","volume":"87","author":"Chen","year":"2015","journal-title":"Anal. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1007\/s12551-012-0076-9","article-title":"Gold nanoclusters as novel optical probes for in vitro and in vivo fluorescence imaging","volume":"4","author":"Shang","year":"2012","journal-title":"Biophys. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.bios.2011.12.007","article-title":"Highly sensitive fluorescent detection of trypsin based on BSA-stabilized gold nanoclusters","volume":"32","author":"Hu","year":"2012","journal-title":"Biosens. Bioelectron."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4683","DOI":"10.1039\/c2nr33202g","article-title":"Disassembly mediated fluorescence recovery of gold nanodots for selective sulfide sensing","volume":"5","author":"Yuan","year":"2013","journal-title":"Nanoscale"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"36582","DOI":"10.1039\/C5RA03449C","article-title":"Label-free detection of Pb2+ based on aggregation induced emission enhancement of Au nanoclusters","volume":"5","author":"Ji","year":"2015","journal-title":"RSC Adv."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1021\/ac8010654","article-title":"Synthesis of fluorescent carbohydrate-protected Au nanodots for detection of concanavalin A and Escherichia coli","volume":"81","author":"Huang","year":"2009","journal-title":"Anal. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"6824","DOI":"10.1002\/anie.200700803","article-title":"Synthesis of highly fluorescent gold nanoparticles for sensing mercury (II)","volume":"46","author":"Huang","year":"2007","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"10057","DOI":"10.1039\/C4DT00490F","article-title":"Fluorescent detection of TNT and 4-nitrophenol by BSA Au nanoclusters","volume":"43","author":"Yang","year":"2014","journal-title":"Dalton Trans."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1021\/ja806804u","article-title":"Protein-directed synthesis of highly fluorescent gold nanoclusters","volume":"131","author":"Xie","year":"2009","journal-title":"J. Am. Chem. Soc."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1039\/C1CC16088E","article-title":"Photoreductive synthesis of water-soluble fluorescent metal nanoclusters","volume":"48","author":"Zhang","year":"2012","journal-title":"Chem. Commun."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"8800","DOI":"10.1021\/nn202860s","article-title":"Synthesis of highly fluorescent metal (Ag, Au, Pt, and Cu) nanoclusters by electrostatically induced reversible phase transfer","volume":"5","author":"Yuan","year":"2011","journal-title":"ACS Nano"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2412","DOI":"10.1021\/ja067727t","article-title":"Etching colloidal gold nanocrystals with hyperbranched and multivalent polymers: A new route to fluorescent and water-soluble atomic clusters","volume":"129","author":"Duan","year":"2007","journal-title":"J. Am. Chem. Soc."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1002\/smll.201201849","article-title":"A galvanic replacement route to prepare strongly fluorescent and highly stable gold nanodots for cellular imaging","volume":"9","author":"Wang","year":"2013","journal-title":"Small"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Tran, M.L., Zvyagin, A.V., and Plakhotnik, T. (2006). Synthesis and spectroscopic observation of dendrimer-encapsulated gold nanoclusters. Chem. Commun., 2400\u20132401.","DOI":"10.1039\/b602079h"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"13069","DOI":"10.1021\/jp203133t","article-title":"Energy transfer between conjugated-oligoelectrolyte-substituted POSS and gold nanocluster for multicolor intracellular detection of mercury ion","volume":"115","author":"Pu","year":"2011","journal-title":"J. Phys. Chem. C"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"6845","DOI":"10.1039\/c2cc32841k","article-title":"Blue emitting gold nanoclusters templated by poly-cytosine DNA at low pH and poly-adenine DNA at neutral pH","volume":"48","author":"Kennedy","year":"2012","journal-title":"Chem. Commun."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1039\/C1JM13457D","article-title":"Microwave-assisted synthesis of BSA-stabilized and HSA-protected gold nanoclusters with red emission","volume":"22","author":"Yan","year":"2012","journal-title":"J. Mater. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"7780","DOI":"10.1021\/ja035473v","article-title":"High quantum yield blue emission from water-soluble Au8 nanodots","volume":"125","author":"Zheng","year":"2003","journal-title":"J. Am. Chem. Soc."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1021\/ja411643u","article-title":"Identification of a highly luminescent Au22(SG)18 nanocluster","volume":"136","author":"Yu","year":"2014","journal-title":"J. Am. Chem. Soc."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.cbpa.2008.01.021","article-title":"Discovering mechanisms of signaling-mediated cysteine oxidation","volume":"12","author":"Poole","year":"2008","journal-title":"Curr. Opin. Chem. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"9638","DOI":"10.1021\/ja802355u","article-title":"A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells","volume":"130","author":"Dickinson","year":"2008","journal-title":"J. Am. Chem. Soc."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"8518","DOI":"10.1021\/ac061483d","article-title":"Picomolar peroxide detection using a chemically activated redox mediator and square wave voltammetry","volume":"78","author":"Lyon","year":"2006","journal-title":"Anal. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2261","DOI":"10.1039\/C3NR05269A","article-title":"Rapid synthesis of highly luminescent and stable Au20 nanoclusters for active tumor-targeted imaging in vitro and in vivo","volume":"6","author":"Zhang","year":"2014","journal-title":"Nanoscale"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"055103","DOI":"10.1088\/0957-4484\/21\/5\/055103","article-title":"Molecular-receptor-specific, non-toxic, near-infrared-emitting Au cluster-protein nanoconjugates for targeted cancer imaging","volume":"21","author":"Retnakumar","year":"2010","journal-title":"Nanotechnology"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2122","DOI":"10.1002\/anie.200806210","article-title":"Color tunability and electrochemiluminescence of silver nanoclusters","volume":"48","author":"Pusa","year":"2009","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"064504","DOI":"10.1117\/1.JNP.6.064504","article-title":"Properties and applications of protein-stabilized fluorescent gold nanoclusters: Short review","volume":"6","author":"Chevrier","year":"2012","journal-title":"J. Nanophotonics"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.1016\/j.bios.2010.08.019","article-title":"Biomolecule-stabilized Au nanoclusters as a fluorescence probe for sensitive detection of glucose","volume":"26","author":"Jin","year":"2011","journal-title":"Biosens. Bioelectron."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"7650","DOI":"10.1039\/C5AN01284H","article-title":"Fenton reaction-mediated fluorescence quenching of N-acetyl-l-cysteine-protected gold nanoclusters: analytical applications of hydrogen peroxide, glucose, and catalase detection","volume":"140","author":"Deng","year":"2015","journal-title":"Analyst"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1039\/C3CC47771A","article-title":"Stable Cu nanoclusters: From an aggregationinduced emission mechanism to biosensing and catalytic applications","volume":"50","author":"Jia","year":"2014","journal-title":"Chem. Commun."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.aca.2012.08.048","article-title":"Polyethyleneimine-capped silver nanoclusters as a fluorescence probe for sensitive detection of hydrogen peroxide and glucose","volume":"749","author":"Wen","year":"2012","journal-title":"Anal. Chim. Acta"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"5563","DOI":"10.1039\/c3an01207g","article-title":"A novel solid-state electrochemiluminescence sensor for the determination of hydrogen peroxide based on an Au nanocluster\u2013silica nanoparticle nanocomposite","volume":"138","author":"Wu","year":"2013","journal-title":"Analyst"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"14303","DOI":"10.1039\/c3ra41755g","article-title":"Preparation of reduced graphene oxide decorated with high density Ag nanorods for non-enzymatic hydrogen peroxide detection","volume":"3","author":"Yu","year":"2013","journal-title":"RSC Adv."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"5803","DOI":"10.1039\/c2an35954e","article-title":"Metal\u2013organic framework templated synthesis of Co3O4 nanoparticles for direct glucose and H2O2 detection","volume":"137","author":"Hou","year":"2012","journal-title":"Analyst"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.aca.2015.02.024","article-title":"Intrinsic peroxidase-like catalytic activity of nitrogen-doped graphene quantum dots and their application in the colorimetric detection of H2O2 and glucose","volume":"869","author":"Lin","year":"2015","journal-title":"Anal. Chim. Acta"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"253","DOI":"10.3390\/chemosensors3040253","article-title":"New nanomaterials and luminescent optical sensors for detection of hydrogen peroxide","volume":"3","author":"Burmistrova","year":"2015","journal-title":"Chemosensors"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1021\/ac1031447","article-title":"Horseradish peroxidase functionalized fluorescent gold nanoclusters for hydrogen peroxide sensing","volume":"83","author":"Wen","year":"2011","journal-title":"Anal. Chem."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/16\/7\/1124\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T19:26:38Z","timestamp":1760210798000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/16\/7\/1124"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7,20]]},"references-count":45,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2016,7]]}},"alternative-id":["s16071124"],"URL":"https:\/\/doi.org\/10.3390\/s16071124","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7,20]]}}}