{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T09:32:31Z","timestamp":1766136751080,"version":"build-2065373602"},"reference-count":85,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Australian Research Council\u2019s Discovery Projects","award":["DP160103910"],"award-info":[{"award-number":["DP160103910"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Optical interrogation of suspicious skin lesions is standard care in the management of skin cancer worldwide. Morphological and functional markers of malignancy are often combined to improve expert human diagnostic power. We propose the evaluation of the combination of two independent optical biomarkers of skin tumours concurrently. The morphological modality of reflectance confocal microscopy (RCM) is combined with the functional modality of laser Doppler flowmetry, which is capable of quantifying tissue perfusion. To realize the idea, we propose laser feedback interferometry as an implementation of RCM, which is able to detect the Doppler signal in addition to the confocal reflectance signal. Based on the proposed technique, we study numerical models of skin tissue incorporating two optical biomarkers of malignancy: (i) abnormal red blood cell velocities and concentrations and (ii) anomalous optical properties manifested through tissue confocal reflectance, using Monte Carlo simulation. We also conduct a laboratory experiment on a microfluidic channel containing a dynamic turbid medium, to validate the efficacy of the technique. We quantify the performance of the technique by examining a signal to background ratio (SBR) in both the numerical and experimental models, and it is shown that both simulated and experimental SBRs improve consistently using this technique. This work indicates the feasibility of an optical instrument, which may have a role in enhanced imaging of skin malignancies.<\/jats:p>","DOI":"10.3390\/s16091411","type":"journal-article","created":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T10:04:28Z","timestamp":1472724268000},"page":"1411","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Concurrent Reflectance Confocal Microscopy and Laser Doppler Flowmetry to Improve Skin Cancer Imaging: A Monte Carlo Model and Experimental Validation"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1421-9946","authenticated-orcid":false,"given":"Alireza","family":"Mowla","sequence":"first","affiliation":[{"name":"School of Information Technology and Electrical Engineering, The University of Queensland, St. Lucia, Brisbane 4072, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Taimre","sequence":"additional","affiliation":[{"name":"School of Mathematics and Physics, The University of Queensland, St. Lucia, Brisbane 4072, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yah","family":"Lim","sequence":"additional","affiliation":[{"name":"School of Information Technology and Electrical Engineering, The University of Queensland, St. Lucia, Brisbane 4072, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1087-2315","authenticated-orcid":false,"given":"Karl","family":"Bertling","sequence":"additional","affiliation":[{"name":"School of Information Technology and Electrical Engineering, The University of Queensland, St. Lucia, Brisbane 4072, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Wilson","sequence":"additional","affiliation":[{"name":"School of Information Technology and Electrical Engineering, The University of Queensland, St. Lucia, Brisbane 4072, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tarl","family":"Prow","sequence":"additional","affiliation":[{"name":"Dermatology Research Centre, The University of Queensland, School of Medicine, Translational Research Institute, Brisbane 4102, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4770-561X","authenticated-orcid":false,"given":"H.","family":"Soyer","sequence":"additional","affiliation":[{"name":"Dermatology Research Centre, The University of Queensland, School of Medicine, Translational Research Institute, Brisbane 4102, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandar","family":"Raki\u0107","sequence":"additional","affiliation":[{"name":"School of Information Technology and Electrical Engineering, The University of Queensland, St. Lucia, Brisbane 4072, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,9,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1046\/j.1365-2133.146.s61.2.x","article-title":"The epidemiology of skin cancer","volume":"146","author":"Diepgen","year":"2002","journal-title":"Br. J. Dermatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1111\/j.1365-2133.2012.10830.x","article-title":"A systematic review of worldwide incidence of nonmelanoma skin cancer","volume":"166","author":"Lomas","year":"2012","journal-title":"Br. J. Dermatol."},{"key":"ref_3","unstructured":"Soyer, H.P., Rigel, D., and Wurm, E.M. (2012). Dermatology, Elsevier."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Baldi, A., Pasquali, P., and Spugnini, E.P. (2014). Skin Cancer: A Practical Approach, Humana Press.","DOI":"10.1007\/978-1-4614-7357-2"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1023\/B:CANC.0000031775.04618.30","article-title":"Aggressive basal cell carcinoma: presentation, pathogenesis, and management","volume":"23","author":"Walling","year":"2004","journal-title":"Cancer Metastasis Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0190-9622(91)70001-I","article-title":"Biology of basal cell carcinoma (Part I)","volume":"24","author":"Miller","year":"1991","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1111\/1523-1747.ep12479191","article-title":"The optics of human skin","volume":"77","author":"Anderson","year":"1981","journal-title":"J. Investig. Dermatol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Salomatina, E., Jiang, B., Novak, J., and Yaroslavsky, A.N. (2006). Optical properties of normal and cancerous human skin in the visible and near-infrared spectral range. J. Biomed. Opt., 11.","DOI":"10.1117\/1.2398928"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"6628","DOI":"10.1364\/AO.38.006628","article-title":"Diffuse reflectance spectroscopy of human adenomatous colon polyps in vivo","volume":"38","author":"Zonios","year":"1999","journal-title":"Appl. Opt."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.1046\/j.0022-202x.2001.01577.x","article-title":"Skin melanin, hemoglobin, and light scattering properties can be quantitatively assessed in vivo using diffuse reflectance spectroscopy","volume":"117","author":"Zonios","year":"2001","journal-title":"J. Investig. Dermatol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1088\/0034-4885\/59\/3\/003","article-title":"Confocal optical microscopy","volume":"59","author":"Webb","year":"1996","journal-title":"Rep. Prog. Phys."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1111\/1523-1747.ep12606215","article-title":"In vivo confocal scanning laser microscopy of human skin: Melanin provides strong contrast","volume":"104","author":"Rajadhyaksha","year":"1995","journal-title":"J. Investig. Dermatol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Hofmann-Wellenhof, R., Pellacani, G., Malvehy, J., and Soyer, H.P. (2012). Reflectance confocal microscopy for skin diseases, Springer.","DOI":"10.1007\/978-3-642-21997-9"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1111\/bjd.12259","article-title":"Is confocal microscopy a valuable tool in diagnosing nodular lesions? A study of 140 cases","volume":"169","author":"Longo","year":"2013","journal-title":"Br. J. Dermatol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1111\/j.1365-2133.2010.10063.x","article-title":"In vivo reflectance confocal microscopy of shave biopsy wounds: Feasibility of intraoperative mapping of cancer margins","volume":"163","author":"Scope","year":"2010","journal-title":"Br. J. Dermatol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1001\/archdermatol.2009.134","article-title":"Reflectance confocal microscopy criteria for squamous cell carcinomas and actinic keratoses","volume":"145","author":"Rishpon","year":"2009","journal-title":"Arch. Dermatol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.jaad.2009.02.014","article-title":"Development of a two-step method for the diagnosis of melanoma by reflectance confocal microscopy","volume":"61","author":"Segura","year":"2009","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.cell.2011.02.013","article-title":"Hallmarks of cancer: The next generation","volume":"144","author":"Hanahan","year":"2011","journal-title":"Cell"},{"key":"ref_19","first-page":"6449","article-title":"Blood flow, oxygen and nutrient supply, and metabolic microenvironment of human tumours: A review","volume":"49","author":"Vaupel","year":"1989","journal-title":"Cancer Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1038\/scientificamerican0708-64sp","article-title":"Taming vessels to treat cancer","volume":"18","author":"Jain","year":"2008","journal-title":"Sci. Am."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1007\/s10103-007-0524-0","article-title":"Review of methodological developments in laser Doppler flowmetry","volume":"24","author":"Rajan","year":"2009","journal-title":"Lasers Med. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/0167-8140(96)01730-6","article-title":"Microregional fluctuations in perfusion within human tumours detected using laser Doppler flowmetry","volume":"40","author":"Pigott","year":"1996","journal-title":"Radiother. Oncol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1046\/j.1365-2230.1999.00428.x","article-title":"Blood flow compared in benign melanocytic naevi, malignant melanomas and basal cell carcinomas","volume":"24","author":"Horstmann","year":"1999","journal-title":"Clin. Exp. Dermatol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1080\/000155502753600849","article-title":"High-resolution laser Doppler perfusion imaging aids in differentiating between benign and malignant melanocytic skin tumours","volume":"82","author":"Esser","year":"2002","journal-title":"Acta Derm Venereol"},{"key":"ref_25","first-page":"184","article-title":"Superficial blood flow following photodynamic therapy of malignant non\u2013melanoma skin tumours measured by laser Doppler perfusion imaging","volume":"136","author":"Wang","year":"1997","journal-title":"Br. J. Dermatol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1136\/ijgc-00009577-200311000-00012","article-title":"A new technique to map vulva microcirculation using laser Doppler perfusion imager","volume":"13","author":"Saravanamuthu","year":"2003","journal-title":"Int. J. Gynecol. Cancer"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1159\/000178963","article-title":"Laser Doppler perfusion imaging\u2013a new technique for measuring breast skin blood flow","volume":"15","author":"Seifalian","year":"1995","journal-title":"Int. J. Microcirc."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1109\/10.222322","article-title":"Laser Doppler perfusion imaging by dynamic light scattering","volume":"40","author":"Jakobsson","year":"1993","journal-title":"IEEE Trans. Biomed. Eng."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1364\/OL.22.001119","article-title":"Noninvasive imaging of in vivo blood flow velocity using optical Doppler tomography","volume":"22","author":"Chen","year":"1997","journal-title":"Opt. Lett."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"014011","DOI":"10.1117\/1.1854173","article-title":"Combining multispectral polarized light imaging and confocal microscopy for localization of nonmelanoma skin cancer","volume":"10","author":"Yaroslavsky","year":"2005","journal-title":"J. Biomed. Opt."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Giuliani, G., Norgia, M., Donati, S., and Bosch, T. (2002). Laser diode self-mixing technique for sensing applications. J. Opt. A Pure Appl. Opt., 4.","DOI":"10.1088\/1464-4258\/4\/6\/371"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1002\/lpor.201100002","article-title":"Developing self-mixing interferometry for instrumentation and measurements","volume":"6","author":"Donati","year":"2012","journal-title":"Laser Photonics Rev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1109\/JSTQE.2013.2270279","article-title":"Self-mixing interferometry for biomedical signals sensing","volume":"20","author":"Donati","year":"2014","journal-title":"IEEE J. Sel. Top. Quantum Electron."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Taimre, T., Nikoli\u0107, M., Bertling, K., Lim, Y.L., Bosch, T., and Raki\u0107, A.D. (2015). Laser Feedback Interferometry: A Guide to the Self-Mixing Effect for Coherent Sensing. Adv. Opt. Photonics, in press.","DOI":"10.1364\/AOP.7.000570"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"694","DOI":"10.3390\/s16050694","article-title":"Current Developments on Optical Feedback Interferometry as an All-Optical Sensor for Biomedical Applications","volume":"16","author":"Perchoux","year":"2016","journal-title":"Sensors"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1364\/OL.18.000238","article-title":"Imaging and vibrational analysis with laser-feedback interferometry","volume":"18","author":"Bearden","year":"1993","journal-title":"Opt. Lett."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2022","DOI":"10.1063\/1.113679","article-title":"Imaging and profiling surface microstructures with noninterferometric confocal laser feedback","volume":"66","author":"Lu","year":"1995","journal-title":"Appl. Phys. Lett."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"11720","DOI":"10.1364\/OE.18.011720","article-title":"Self-mixing flow sensor using a monolithic VCSEL array with parallel readout","volume":"18","author":"Lim","year":"2010","journal-title":"Opt. Express"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/s10404-012-1029-0","article-title":"Flow profile measurement in microchannel using the optical feedback interferometry sensing technique","volume":"14","author":"Campagnolo","year":"2013","journal-title":"Microfluid. Nanofluid."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Mowla, A., Nikolic, M., Lim, Y.L., Bertling, K., Rakic, A.D., and Taimre, T. (2014, January 14\u201317). Effect of the optical numerical aperture on the Doppler spectrum in laser Doppler velocimetry. Proceedings of the 2014 IEEE Conference onOptoelectronic and Microelectronic Materials & Devices, Perth, Australia.","DOI":"10.1109\/COMMAD.2014.7038654"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1364\/AO.54.000018","article-title":"Effect of the optical system on the Doppler spectrum in laser-feedback interferometry","volume":"54","author":"Mowla","year":"2015","journal-title":"Appl. Opt."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-60761-847-8_1","article-title":"Reflecting on confocal microscopy: A personal perspective","volume":"1075","author":"White","year":"2014","journal-title":"Confocal Microsc. Methods Protoc."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1002\/sca.4950100403","article-title":"Memoir on inventing the confocal scanning microscope","volume":"10","author":"Minsky","year":"1988","journal-title":"Scanning"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1083\/jcb.105.1.41","article-title":"An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy","volume":"105","author":"White","year":"1987","journal-title":"J. Cell Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1364\/AO.33.000578","article-title":"Semiconductor laser confocal microscopy","volume":"33","author":"Rea","year":"1994","journal-title":"Appl. Opt."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.optcom.2004.04.052","article-title":"Self-mixing microscopic interferometer for the measurement of microprofile","volume":"238","author":"Wang","year":"2004","journal-title":"Opt. Commun."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Tan, Y., Wang, W., Xu, C., and Zhang, S. (2013). Laser confocal feedback tomography and nano-step height measurement. Sci. Rep., 3.","DOI":"10.1038\/srep02971"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Mowla, A., Taimre, T., Lim, Y.L., Bertling, K., Wilson, S.J., Prow, T.W., Soyer, H.P., and Raki\u0107, A.D. (2016). Diffuse reflectance imaging for non-melanoma skin cancer detection using laser feedback interferometry. Proc. SPIE, 9887.","DOI":"10.1117\/12.2227449"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Michalzik, R. (2012). VCSELs: Fundamentals, Technology and Applications of Vertical-Cavity Surface-Emitting Lasers, Springer.","DOI":"10.1007\/978-3-642-24986-0"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Albrecht, H.E. (2003). Laser Doppler and Phase Doppler Measurement Techniques, Springer.","DOI":"10.1007\/978-3-662-05165-8"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/s11517-012-0973-x","article-title":"Self-mixing microprobe for monitoring microvascular perfusion in rat brain","volume":"51","author":"Figueiras","year":"2013","journal-title":"Med. Biol. Eng. Comput."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0169-2607(95)01640-F","article-title":"MCML\u2013Monte Carlo modeling of light transport in multi-layered tissues","volume":"47","author":"Wang","year":"1995","journal-title":"Comput. Methods Progr. Biomed."},{"key":"ref_53","unstructured":"Tuchin, V. (2007). Tissue Optics: Light Scattering Methods and Instruments for Medical Diagnosis, SPIE Press."},{"key":"ref_54","unstructured":"Kroese, D.P., Taimre, T., and Botev, Z.I. (2013). Handbook of Monte Carlo Methods, John Wiley & Sons."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"6676","DOI":"10.1364\/AO.41.006676","article-title":"Derivation of a Monte Carlo method for modeling heterodyne detection in optical coherence tomography systems","volume":"41","author":"Tycho","year":"2002","journal-title":"Appl. Opt."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"014015","DOI":"10.1117\/1.2854691","article-title":"Optical microcirculatory skin model: Assessed by Monte Carlo simulations paired with in vivo laser Doppler flowmetry","volume":"13","author":"Fredriksson","year":"2008","journal-title":"J. Biomed. Opt."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1364\/AO.12.000555","article-title":"Optical constants of water in the 200-nm to 200-\u03bcm wavelength region","volume":"12","author":"Hale","year":"1973","journal-title":"Appl. Opt."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Hammer, M., Yaroslavsky, A.N., and Schweitzer, D. (2001). A scattering phase function for blood with physiological haematocrit. Phys. Med. Biol., 46.","DOI":"10.1088\/0031-9155\/46\/3\/402"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1002\/jbio.201400105","article-title":"Wavelength optimized cross-polarized wide-field imaging for noninvasive and rapid evaluation of dermal structures","volume":"8","author":"Feng","year":"2015","journal-title":"J. Biophotonics"},{"key":"ref_60","first-page":"1","article-title":"Skin optics","volume":"1998","author":"Jacques","year":"1998","journal-title":"Or. Med. Laser Center News"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Mowla, A., Taimre, T., Lim, Y.L., Bertling, K., Wilson, S.J., Prow, T.W., and Raki\u0107, A.D. (2016). A Compact Laser Imaging System for Concurrent Reflectance Confocal Microscopy and Laser Doppler Flowmetry. IEEE Photonics J., in press.","DOI":"10.1109\/JPHOT.2016.2599014"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"5844","DOI":"10.1364\/AO.31.005844","article-title":"Self-mixing laser-Doppler velocimetry of liquid flow and of blood perfusion in tissue","volume":"31","author":"Koelink","year":"1992","journal-title":"Appl. Opt."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"3690","DOI":"10.1364\/OL.36.003690","article-title":"Maintaining maximum signal-to-noise ratio in uncooled vertical-cavity surface-emitting laser-based self-mixing sensors","volume":"36","author":"Matharu","year":"2011","journal-title":"Opt. Lett."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1002\/lsm.1900120510","article-title":"Optical properties of Intralipid: A phantom medium for light propagation studies","volume":"12","author":"Flock","year":"1992","journal-title":"Lasers Surg. Med."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Cubeddu, R., Pifferi, A., Taroni, P., Torricelli, A., and Valentini, G. (1997). A solid tissue phantom for photon migration studies. Phys. Med. Biol., 42.","DOI":"10.1088\/0031-9155\/42\/10\/011"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1937","DOI":"10.1109\/JSEN.2015.2507184","article-title":"Simple electrical modulation scheme for laser feedback imaging","volume":"16","author":"Bertling","year":"2016","journal-title":"IEEE Sens. J."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1111\/j.1751-1097.1991.tb08470.x","article-title":"In vivo spectrophotometric evaluation of neoplastic and non-neoplastic skin pigmented lesions\u2013I. reflectance measurements","volume":"53","author":"Marchesini","year":"1991","journal-title":"Photochem. Photobiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/S1011-1344(97)00113-9","article-title":"Spectrophotometric imaging of cutaneous pigmented lesions: Discriminant analysis, optical properties and histological characteristics","volume":"42","author":"Tomatis","year":"1998","journal-title":"J. Photochem. Photobiol. B Biol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"8661","DOI":"10.1364\/OE.14.008661","article-title":"Modeling diffuse reflectance from semi-infinite turbid media: Application to the study of skin optical properties","volume":"14","author":"Zonios","year":"2006","journal-title":"Opt. Express"},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Zonios, G., Dimou, A., Bassukas, I., Galaris, D., Tsolakidis, A., and Kaxiras, E. (2008). Melanin absorption spectroscopy: New method for noninvasive skin investigation and melanoma detection. J. Biomed. Opt., 13.","DOI":"10.1117\/1.2844710"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1111\/j.1751-1097.2009.00630.x","article-title":"In vivo optical properties of melanocytic skin lesions: Common nevi, dysplastic nevi and malignant melanoma","volume":"86","author":"Zonios","year":"2010","journal-title":"Photochem. Photobiol."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Garcia-Uribe, A., Smith, E.B., Zou, J., Duvic, M., Prieto, V., and Wang, L.V. (2011). In-vivo characterization of optical properties of pigmented skin lesions including melanoma using oblique incidence diffuse reflectance spectrometry. J. Biomed. Opt., 16.","DOI":"10.1117\/1.3536509"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"2738","DOI":"10.1158\/0008-5472.CAN-11-4027","article-title":"In vivo diagnosis of melanoma and nonmelanoma skin cancer using oblique incidence diffuse reflectance spectrometry","volume":"72","author":"Zou","year":"2012","journal-title":"Cancer Res."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"3523","DOI":"10.1091\/mbc.e06-10-0973","article-title":"The size of the nucleus increases as yeast cells grow","volume":"18","author":"Jorgensen","year":"2007","journal-title":"Mol. Biol. Cell"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1111\/j.1365-2303.2005.00271.x","article-title":"Proposed Sheffield quantitative criteria in cervical cytology to assist the grading of squamous cell dyskaryosis, as the British Society for Clinical Cytology definitions require amendment","volume":"16","author":"Slater","year":"2005","journal-title":"Cytopathology"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1002\/1097-0142(19950115)75:2+<645::AID-CNCR2820751405>3.0.CO;2-1","article-title":"Precursors to skin cancer","volume":"75","author":"Sober","year":"1995","journal-title":"Cancer"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1111\/j.1468-3083.2008.02579.x","article-title":"Actinic keratosis: An occupational and environmental disorder","volume":"22","author":"Schwartz","year":"2008","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1046\/j.1365-2133.146.s61.5.x","article-title":"Carcinogenesis of basal cell carcinomas: Genetics and molecular mechanisms","volume":"146","author":"Lacour","year":"2002","journal-title":"Br. J. Dermatol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1002\/1097-0142(195309)6:5<963::AID-CNCR2820060515>3.0.CO;2-Q","article-title":"Field cancerization in oral stratified squamous epithelium. Clinical implications of multicentric origin","volume":"6","author":"Slaughter","year":"1953","journal-title":"Cancer"},{"key":"ref_80","first-page":"3604","article-title":"p53 mutations in nonmelanoma skin cancer of the head and neck: Molecular evidence for field cancerization","volume":"55","author":"Kanjilal","year":"1995","journal-title":"Cancer Res."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1067\/mjd.2001.110395","article-title":"Automatic differentiation of melanoma from melanocytic nevi with multispectral digital dermoscopy: A feasibility study","volume":"44","author":"Elbaum","year":"2001","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1111\/srt.12197","article-title":"Vascular patterns of non-pigmented tumoural skin lesions: Confocal perspectives","volume":"21","author":"Incel","year":"2015","journal-title":"Skin Res. Technol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1046\/j.1523-1747.2003.12372.x","article-title":"Demarcation of nonmelanoma skin cancer margins in thick excisions using multispectral polarized light imaging","volume":"121","author":"Yaroslavsky","year":"2003","journal-title":"J. Investig. Dermatol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/j.1365-2133.1992.tb16981.x","article-title":"The measurement of photodamage","volume":"127","author":"Marks","year":"1992","journal-title":"Br. J. Dermatol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1111\/j.1751-1097.2008.00443.x","article-title":"Reflectance confocal microscopy for in vivo skin imaging","volume":"84","author":"Longo","year":"2008","journal-title":"Photochem. 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