{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:21:50Z","timestamp":1760242910401,"version":"build-2065373602"},"reference-count":49,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2016,10,12]],"date-time":"2016-10-12T00:00:00Z","timestamp":1476230400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21475123","21505011"],"award-info":[{"award-number":["21475123","21505011"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Zr(IV) can form phosphate and Zr(IV) (\u2013PO32\u2212\u2013Zr4+\u2013) complex owing to the high affinity between Zr(IV) with phosphate. Zr(IV) can induce the aggregation of gold nanoparticles (AuNPs), while adenosine triphosphate(ATP) can prevent Zr(IV)-induced aggregation of AuNPs. Herein, a visual and plasmon resonance absorption (PRA)sensor for ATP have been developed using AuNPs based on the high affinity between Zr(IV)with ATP. AuNPs get aggregated in the presence of certain concentrations of Zr(IV). After the addition of ATP, ATP reacts with Zr(IV) and prevents AuNPs from aggregation, enabling the detection of ATP. Because of the fast interaction of ATP with Zr(IV), ATP can be detected with a detection limit of 0.5 \u03bcM within 2 min by the naked eye. Moreover, ATP can be detected by the PRA technique with higher sensitivity. The A520nm\/A650nm values in PRA spectra increase linearly with the concentrations of ATP from 0.1 \u03bcM to 15 \u03bcM (r = 0.9945) with a detection limit of 28 nM. The proposed visual and PRA sensor exhibit good selectivity against adenosine, adenosine monophosphate, guanosine triphosphate, cytidine triphosphate and uridine triphosphate. The recoveries for the analysis of ATP in synthetic samples range from 95.3% to 102.0%. Therefore, the proposed novel sensor for ATP is promising for real-time or on-site detection of ATP.<\/jats:p>","DOI":"10.3390\/s16101674","type":"journal-article","created":{"date-parts":[[2016,10,12]],"date-time":"2016-10-12T10:18:49Z","timestamp":1476267529000},"page":"1674","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Visual and Plasmon Resonance Absorption Sensor for Adenosine Triphosphate Based on the High Affinity between Phosphate and Zr(IV)"],"prefix":"10.3390","volume":"16","author":[{"given":"Wenjing","family":"Qi","sequence":"first","affiliation":[{"name":"State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China"},{"name":"Chongqing Key Laboratory of Green Synthesis and Applications, College of Chemistry, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongyuan","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"Halawa","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China"},{"name":"Chinese Academy of Sciences, University of Chinese Academy of Sciences, No. 19A Yuquanlu, Beijing 100049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guobao","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,10,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.bios.2015.05.043","article-title":"High-sensitivity detection of ATP using a localized surface plasmon resonance (LSPR) sensor and split aptamers","volume":"73","author":"Park","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.bios.2014.07.022","article-title":"A label-free aptasensor for highly sensitive detection of ATP and thrombin based on metal-enhanced PicoGreen fluorescence","volume":"63","author":"Wang","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.bios.2014.07.064","article-title":"An exonuclease I-based label-free fluorometric aptasensor for adenosine triphosphate (ATP) detection with a wide concentration range","volume":"63","author":"Wei","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.bios.2014.07.070","article-title":"A cost-effective Z-folding controlled liquid handling microfluidic paper analysis device for pathogen detection via ATP quantification","volume":"63","author":"Jin","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.bios.2014.07.007","article-title":"Highly selective and sensitive electrochemical biosensor for ATP based on the dual strategy integrating the cofactor-dependent enzymatic ligation reaction with self-cleaving DNAzyme-amplified electrochemical detection","volume":"63","author":"Lu","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1113\/expphysiol.2014.078873","article-title":"The human carotid body releases acetylcholine, ATP and cytokines during hypoxia","volume":"99","author":"Kahlin","year":"2014","journal-title":"Exp. Physiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.ab.2007.08.003","article-title":"A novel kinase-based ATP assay using molecular beacon","volume":"372","author":"Ma","year":"2008","journal-title":"Anal. Biochem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1538","DOI":"10.1039\/c2an15999f","article-title":"A label-free G-quadruplex-based switch-on fluorescence assay for the selective detection of ATP","volume":"137","author":"He","year":"2012","journal-title":"Analyst"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4256","DOI":"10.1039\/C5CC00037H","article-title":"Label-free signal-on ATP aptasensor based on the remarkable quenching of tris(2,2\u2032-bipyridine)ruthenium(II) electrochemiluminescence by single-walled carbon nanohorn","volume":"51","author":"Liu","year":"2015","journal-title":"Chem. Commun."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"9807","DOI":"10.1021\/ac901925x","article-title":"[Ru(bpy)2dppz]2+ electrochemiluminescence switch and Its applications for DNA interaction study and label-free ATP aptasensor","volume":"81","author":"Hu","year":"2009","journal-title":"Anal. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"13356","DOI":"10.1002\/chem.201001736","article-title":"Label-free and signal-on electrochemiluminescence aptasensor for ATP based on target-induced linkage of split aptamer fragments by using [Ru(phen)3]2+ intercalated into double-strand DNA as a probe","volume":"16","author":"Liu","year":"2010","journal-title":"Chem. Eur. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.bios.2011.09.047","article-title":"Quantum dots-based multifunctional dendritic superstructure for amplified electrochemiluminescence detection of ATP","volume":"31","author":"Jie","year":"2012","journal-title":"Biosens. Bioelectron."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1039\/b9nr00393b","article-title":"DNA aptasensor for the detection of ATP based on quantum dots electrochemiluminescence","volume":"2","author":"Huang","year":"2010","journal-title":"Nanoscale"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"7659","DOI":"10.1039\/c4cc01458h","article-title":"Ultrasensitive detection of ATP based on ATP regeneration amplification and its application in cell homogenate and human serum","volume":"50","author":"Guo","year":"2014","journal-title":"Chem. Commun."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"23900","DOI":"10.1039\/c2jm35379b","article-title":"Porphyrin functionalized graphene nanosheets-based electrochemical aptasensor for label-free ATP detection","volume":"22","author":"Zhang","year":"2012","journal-title":"J. Mater. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1016\/j.talanta.2009.01.008","article-title":"Microbiosensor based on glucose oxidase and hexokinase co-immobilised on platinum microelectrode for selective ATP detection","volume":"78","author":"Soldatkin","year":"2009","journal-title":"Talanta"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2101","DOI":"10.1016\/j.bios.2010.02.007","article-title":"A sensitive ligase-based ATP electrochemical assay using molecular beacon-like DNA","volume":"25","author":"Wang","year":"2010","journal-title":"Biosens. Bioelectron."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.aca.2014.12.049","article-title":"A sensitive electrochemical aptasensor for ATP detection based on exonuclease III-assisted signal amplification strategy","volume":"862","author":"Bao","year":"2015","journal-title":"Anal. Chim. Acta"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"15411","DOI":"10.1039\/C4CC08044K","article-title":"A ratiometric fluorescent probe with unexpected high selectivity for ATP and its application in cell imaging","volume":"50","author":"Tang","year":"2014","journal-title":"Chem. Commun."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1662","DOI":"10.1039\/c2ay25166c","article-title":"Graphene oxide as a nano-platform for ATP detection based on aptamer chemistry","volume":"4","author":"Pu","year":"2012","journal-title":"Anal. Methods"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.ab.2013.10.021","article-title":"A target-triggered strand displacement reaction cycle: The design and application in adenosine triphosphate sensing","volume":"446","author":"Cheng","year":"2014","journal-title":"Anal. Biochem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.bios.2013.08.002","article-title":"Target-induced structure switching of hairpin aptamers for label-free and sensitive fluorescent detection of ATP via exonuclease-catalyzed target recycling amplification","volume":"51","author":"Xu","year":"2014","journal-title":"Biosens. Bioelectron."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"5029","DOI":"10.1002\/chem.201203402","article-title":"Label-free colorimetric detection of arsenite utilizing G-\/T-rich oligonucleotides and unmodified Au nanoparticles","volume":"19","author":"Liang","year":"2013","journal-title":"Chem. Eur. J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3997","DOI":"10.1039\/c2ay25403d","article-title":"A silver-specific DNA-based bio-assay for Ag(\u0399) detection via the aggregation of unmodified gold nanoparticles in aqueous solution coupled with resonance Rayleigh scattering","volume":"4","author":"Zhan","year":"2012","journal-title":"Anal. Methods"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2251","DOI":"10.1007\/s00604-016-1861-0","article-title":"A label-free colorimetric progesterone aptasensor based on the aggregation of gold nanoparticles","volume":"183","author":"Du","year":"2016","journal-title":"Microchim. Acta"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"17424","DOI":"10.1039\/c3ra42138d","article-title":"Sensitive colorimetric detection of melamine in milk with an aptamer-modified nanogold probe","volume":"3","author":"Xing","year":"2013","journal-title":"RSC Adv."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2077","DOI":"10.1039\/c0cc05171c","article-title":"Rapid naked-eye detection of mercury ions based on non-crosslinking aggregation of double-stranded DNA-carrying gold nanoparticles","volume":"47","author":"Kanayama","year":"2011","journal-title":"Chem. Commun."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5022","DOI":"10.1021\/es9001983","article-title":"A localized surface plasmon rsonance light-scattering assay of mercury (II) on the basis of Hg2+-DNA complex induced aggregation of gold nanoparticles","volume":"43","author":"Liu","year":"2009","journal-title":"Environ. Sci. Technol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4459","DOI":"10.1039\/c2cc30384a","article-title":"Cationic polymers and aptamers mediated aggregation of gold nanoparticles for the colorimetric detection of arsenic(III) in aqueous solution","volume":"48","author":"Wu","year":"2012","journal-title":"Chem. Commun."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2266","DOI":"10.1039\/c2ay25596k","article-title":"A highly sensitive resonance scattering based sensor using unmodified gold nanoparticles for daunomycin detection in aqueous solution","volume":"4","author":"He","year":"2012","journal-title":"Anal. Methods"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3242","DOI":"10.1039\/c3ay40175h","article-title":"Responsive disassembly of the gold nanoparticle aggregates triggered by the competitive adsorption for lighting up the colorimetric sensing","volume":"5","author":"Liu","year":"2013","journal-title":"Anal. Methods"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"15078","DOI":"10.3390\/s121115078","article-title":"Simple, fast and selective detection of adenosine triphosphate at physiological pH using unmodified gold nanoparticles as colorimetric probes and metal ions as cross-linkers","volume":"12","author":"Deng","year":"2012","journal-title":"Sensors"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.bios.2015.11.043","article-title":"A sensitive aptasensor for colorimetric detection of adenosine triphosphate based on the protective effect of ATP-aptamer complexes on unmodified gold nanoparticles","volume":"78","author":"Huo","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4251","DOI":"10.1021\/ac200525a","article-title":"Study of Single-Stranded DNA Binding Protein-Nucleic Acids Interactions using Unmodified Gold Nanoparticles and Its Application for Detection of Single Nucleotide Polymorphisms","volume":"83","author":"Tan","year":"2011","journal-title":"Anal. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.snb.2011.03.077","article-title":"Aptamer-based colorimetric biosensing of dopamine using unmodified gold nanoparticles","volume":"156","author":"Zheng","year":"2011","journal-title":"Sens. Actuators B Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1016\/j.bios.2008.02.008","article-title":"Colorimetric determination of urinary adenosine using aptamer-modified gold nanoparticles","volume":"23","author":"Chen","year":"2008","journal-title":"Biosens. Bioelectron."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"9148","DOI":"10.1021\/ac401807b","article-title":"Using graphene quantum dots as photoluminescent probes for protein kinase sensing","volume":"85","author":"Wang","year":"2013","journal-title":"Anal. Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"6274","DOI":"10.1039\/c2cc31641b","article-title":"Synthesis of adenosine functionalized metal immobilized magnetic nanoparticles for highly selective and sensitive enrichment of phosphopeptides","volume":"48","author":"Zhang","year":"2012","journal-title":"Chem. Commun."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/j.bios.2011.10.035","article-title":"Highly sensitive protein kinase activity assay based on electrochemiluminescence nanoprobes","volume":"31","author":"Zhao","year":"2012","journal-title":"Biosens. Bioelectron."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4893","DOI":"10.1039\/c0cc00886a","article-title":"Visual and light scattering spectrometric detections of melamine with polythymine-stabilized gold nanoparticles through specific triple hydrogen-bonding recognition","volume":"46","author":"Qi","year":"2010","journal-title":"Chem. Commun."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.aca.2013.05.030","article-title":"Visual and surface plasmon resonance sensor for zirconium based on zirconium-induced aggregation of adenosine triphosphate-stabilized gold nanoparticles","volume":"787","author":"Qi","year":"2013","journal-title":"Anal. Chim. Acta"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"12637","DOI":"10.1002\/chem.201201914","article-title":"Visual Detection of Glucose Using Conformational Switch of I-Motif DNA and Non-Crosslinking Gold Nanoparticles","volume":"18","author":"Li","year":"2012","journal-title":"Chem. Eur. J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.aca.2015.12.040","article-title":"Molecular structure and thermodynamic predictions to create highly sensitive microRNA biosensors","volume":"909","author":"Larkey","year":"2016","journal-title":"Anal. Chim. Acta"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"9676","DOI":"10.1021\/ac202610e","article-title":"Copper quantum clusters in protein matrix: Potential sensor of Pb2+ ion","volume":"83","author":"Goswami","year":"2011","journal-title":"Anal. Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"11595","DOI":"10.1021\/ja4035939","article-title":"A dual-emission fluorescent nanocomplex of gold-cluster-decorated silica particles for live cell imaging of highly reactive oxygen species","volume":"135","author":"Chen","year":"2013","journal-title":"J. Am. Chem. Soc."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2883","DOI":"10.1021\/ac1032403","article-title":"Fluorescent Ag clusters via a protein-directed approach as a Hg(II) ion sensor","volume":"83","author":"Guo","year":"2011","journal-title":"Anal. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"7143","DOI":"10.1021\/la7006843","article-title":"Tunable stabilization of gold nanoparticles in aqueous solutions by mononucleotides","volume":"23","author":"Zhao","year":"2007","journal-title":"Langmuir"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"9284","DOI":"10.1039\/c2cc34279k","article-title":"GOx signaling triggered by aptamer-based ATP detection","volume":"48","author":"Sitaula","year":"2012","journal-title":"Chem. Commun."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/1011-1344(94)07078-3","article-title":"Irradiation with He-Ne laser increases ATP level in cells cultivated in vitro","volume":"27","author":"Karu","year":"1995","journal-title":"J. Photochem. Photobiol. 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