{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T03:19:25Z","timestamp":1784949565696,"version":"3.55.0"},"reference-count":23,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2016,11,23]],"date-time":"2016-11-23T00:00:00Z","timestamp":1479859200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Fluorescence detecting of exogenous EpCAM (epithelial cell adhesion molecule) or muc1 (mucin1) expression correlated to cancer metastasis using nanoparticles provides pivotal information on CTC (circulating tumor cell) occurrence in a noninvasive tool. In this study, we study a new skill to detect extracellular EpCAM\/muc1 using quantum dot-based aptamer beacon (QD-EpCAM\/muc1 ALB (aptamer linker beacon). The QD-EpCAM\/muc1 ALB was designed using QDs (quantum dots) and probe. The EpCAM\/muc1-targeting aptamer contains a Ep-CAM\/muc1 binding sequence and BHQ1 (black hole quencher 1) or BHQ2 (black hole quencher2). In the absence of target EpCAM\/muc1, the QD-EpCAM\/muc1 ALB forms a partial duplex loop-like aptamer beacon and remained in quenched state because the BHQ1\/2 quenches the fluorescence signal-on of the QD-EpCAM\/muc1 ALB. The binding of EpCAM\/muc1 of CTC to the EpCAM\/muc1 binding aptamer sequence of the EpCAM\/muc1-targeting oligonucleotide triggered the dissociation of the BHQ1\/2 quencher and subsequent signal-on of a green\/red fluorescence signal. Furthermore, acute inflammation was stimulated by trigger such as caerulein in vivo, which resulted in increased fluorescent signal of the cy5.5-EpCAM\/muc1 ALB during cancer metastasis due to exogenous expression of EpCAM\/muc1 in Panc02-implanted mouse model.<\/jats:p>","DOI":"10.3390\/s16111909","type":"journal-article","created":{"date-parts":[[2016,11,23]],"date-time":"2016-11-23T11:12:14Z","timestamp":1479899534000},"page":"1909","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Optical Aptamer Probes of Fluorescent Imaging to Rapid Monitoring of Circulating Tumor Cell"],"prefix":"10.3390","volume":"16","author":[{"given":"Ji","family":"Hwang","sequence":"first","affiliation":[{"name":"Preclinical Research Center, Biomedical Research Institute, Seoul National University Bundang Hospital, 82, Gumi-ro 173beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do 13620, Korea"},{"name":"The Institute for the 3Rs, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Surgery, Seoul National University Bundang Hospital, 82, Gumi-ro 173beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do 13620, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ho-Seong","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Surgery, Seoul National University Bundang Hospital, 82, Gumi-ro 173beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do 13620, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyunggon","family":"Kim","sequence":"additional","affiliation":[{"name":"Asan Institute for Life Sciences, Asan Medical Center, Seoul, Department of Medicine, University of Ulsan College of Medicine, 43 gil Olympic-ro, Pungnap dong, Songpa gu, Seoul 138-736, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Han","sequence":"additional","affiliation":[{"name":"The Institute for the 3Rs, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2016,11,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.cell.2011.11.025","article-title":"EMT and dissemination precede pancreatic tumor formation","volume":"148","author":"Rhim","year":"2012","journal-title":"Cell"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1093\/jnci\/djn419","article-title":"Anti-epithelial cell adhesion molecule antibodies and the detection of circulating normal-like breast tumor cells","volume":"101","author":"Sieuwerts","year":"2009","journal-title":"J. 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