{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T13:54:41Z","timestamp":1780494881289,"version":"3.54.1"},"reference-count":27,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2016,12,16]],"date-time":"2016-12-16T00:00:00Z","timestamp":1481846400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this study, we developed lateral flow assay (LFA) biosensors for the detection of hepatitis B surface antigens using well-controlled gold nanoparticles (AuNPs). To enhance colorimetric signals, a seeded growth method was used for the preparation of size-controlled AuNPs with a narrow size distribution. Different sizes of AuNPs in the range of 342\u2013137.8 nm were conjugated with antibodies and then optimized for the efficient detection of LFA biosensors. The conjugation stability was investigated by UV-vis spectroscopy of AuNP dispersion at various pH values and concentrations of antibody. Based on optimized conjugation conditions, the use of 42.7 \u00b1 0.8 nm AuNPs exhibited superior performance for the detection of LFAs relative to other sizes of AuNPs.<\/jats:p>","DOI":"10.3390\/s16122154","type":"journal-article","created":{"date-parts":[[2016,12,16]],"date-time":"2016-12-16T10:55:28Z","timestamp":1481885728000},"page":"2154","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":84,"title":["Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen"],"prefix":"10.3390","volume":"16","author":[{"given":"Dong","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering, Kangwon National University, Samcheok 25913, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Kim","sequence":"additional","affiliation":[{"name":"Nanobio Application Team, National NanoFab Center (NNFC), Daejeon 34141, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seok","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Kangwon National University, Samcheok 25913, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinwoon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon 22212, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nam","family":"Heo","sequence":"additional","affiliation":[{"name":"Department of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon 22212, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moon-Keun","family":"Lee","sequence":"additional","affiliation":[{"name":"Nanobio Application Team, National NanoFab Center (NNFC), Daejeon 34141, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seok","family":"Lee","sequence":"additional","affiliation":[{"name":"Nanobio Application Team, National NanoFab Center (NNFC), Daejeon 34141, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Byeong","family":"Kim","sequence":"additional","affiliation":[{"name":"Research and Development Center, Tomorrow &amp; Solution (T&amp;S), E19-604, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In","family":"Kim","sequence":"additional","affiliation":[{"name":"Research Center 2, Eudipia Inc., Cheongju 361-951, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1612-4473","authenticated-orcid":false,"given":"Yun","family":"Huh","sequence":"additional","affiliation":[{"name":"Department of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon 22212, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bong","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Kangwon National University, Samcheok 25913, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2016,12,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7634","DOI":"10.1021\/nn3034056","article-title":"Hybrid nanocluster plasmonic resonator for immunological detection of hepatitis B virus","volume":"6","author":"Draz","year":"2012","journal-title":"ACS Nano"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"5173","DOI":"10.1021\/ac504832c","article-title":"Rapid fluorescent lateral-flow immunoassay for hepatitis B virus genotyping","volume":"87","author":"Song","year":"2015","journal-title":"Anal. 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