{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:25:22Z","timestamp":1761513922203,"version":"build-2065373602"},"reference-count":32,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2016,12,16]],"date-time":"2016-12-16T00:00:00Z","timestamp":1481846400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21305091"],"award-info":[{"award-number":["21305091"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"General Administration of Quality Supervision, Inspection and Quarantine of the People\u2019s Republic of China","award":["2014QK141"],"award-info":[{"award-number":["2014QK141"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Pb(II) can cause serious damaging effects to human health, and thus, the study of Pb2+ detection methods to sensitively and selectively monitor Pb(II) pollution has significant importance. In this work, we have developed a label-free fluorescence sensing strategy based on a Pb(II) DNAzyme cleavage and the ThT\/G-quadruplex complex. In the presence of Pb(II), a G-rich tail was cut and released from the substrate strand, which then would form a G-quadruplex structure by combination with ThT dye. The fluorescence signal increase was then measured for sensitive Pb(II) quantification with a limit of detection of 0.06 nM. Our sensor also demonstrated high selectivity against six different metal ions, which is very important for the analysis of complex samples.<\/jats:p>","DOI":"10.3390\/s16122155","type":"journal-article","created":{"date-parts":[[2016,12,16]],"date-time":"2016-12-16T10:55:28Z","timestamp":1481885728000},"page":"2155","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["A Sensitive and Label-Free Pb(II) Fluorescence Sensor Based on a DNAzyme Controlled G-Quadruplex\/Thioflavin T Conformation"],"prefix":"10.3390","volume":"16","author":[{"given":"Yanli","family":"Wen","sequence":"first","affiliation":[{"name":"Biometrology Laboratory, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lele","family":"Wang","sequence":"additional","affiliation":[{"name":"Biometrology Laboratory, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lanying","family":"Li","sequence":"additional","affiliation":[{"name":"Biometrology Laboratory, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Xu","sequence":"additional","affiliation":[{"name":"Biometrology Laboratory, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Liu","sequence":"additional","affiliation":[{"name":"Biometrology Laboratory, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,12,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.snb.2015.12.006","article-title":"Curcumin based biocompatible nanofibers for lead ion detection","volume":"226","author":"Raj","year":"2016","journal-title":"Sens. Actuators B Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"5979","DOI":"10.1039\/C5CC00288E","article-title":"DNAzyme tunable lead (II) gating based on ion-track etched conical nanochannels","volume":"51","author":"Shang","year":"2015","journal-title":"Chem. Commun."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"6642","DOI":"10.1021\/ja034775u","article-title":"A colorimetric lead biosensor using DNAzyme-directed assembly of gold nanoparticles","volume":"125","author":"Liu","year":"2003","journal-title":"J. Am. Chem. Soc."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"10107","DOI":"10.1021\/ja051075b","article-title":"A highly selective fluorescent chemosensor for Pb2+","volume":"127","author":"Kwon","year":"2005","journal-title":"J. Am. Chem. Soc."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"6278","DOI":"10.1039\/c1cc11486g","article-title":"Metal ion-modulated graphene-DNAzyme interactions: Design of a nanoprobe for fluorescent detection of lead(II) ions with high sensitivity, selectivity and tunable dynamic range","volume":"47","author":"Wen","year":"2011","journal-title":"Chem. Commun."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.foodchem.2015.04.061","article-title":"Application of solvent-assisted dispersive solid phase extraction as a new, fast, simple and reliable preconcentration and trace detection of lead and cadmium ions in fruit and water samples","volume":"187","author":"Behbahani","year":"2015","journal-title":"Food Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/j.talanta.2015.10.023","article-title":"Direct extraction of lead (II) from untreated human blood serum using restricted access carbon nanotubes and its determination by atomic absorption spectrometry","volume":"147","author":"Barbosa","year":"2016","journal-title":"Talanta"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0003-2670(94)85005-4","article-title":"Determination of cadmium, copper and lead in environmental samples. An evaluation of flow injection on-line sorbent extraction for flame atomic absorption spectrometry","volume":"285","author":"Ma","year":"1994","journal-title":"Anal. Chim. Acta"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.chemgeo.2004.06.017","article-title":"The application of laser ablation-inductively coupled plasma-mass spectrometry to in situ U-Pb zircon geochronology","volume":"211","author":"Jackson","year":"2004","journal-title":"Chem. Geol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/S0039-9140(99)00318-5","article-title":"Determination of trace elements in granites by inductively coupled plasma mass spectrometry","volume":"51","author":"Liang","year":"2000","journal-title":"Talanta"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1039\/a706969c","article-title":"Determination of organic lead in soils and waters by hydride generation inductively coupled plasma atomic emission spectrometry","volume":"123","author":"Elfering","year":"1998","journal-title":"Analyst"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1007\/s002160100769","article-title":"Comparison of inductively coupled plasma-atomic emission spectrometry, anodic stripping voltammetry and instrumental neutron-activation analysis for the determination of heavy metals in airborne particulate matter","volume":"369","year":"2001","journal-title":"Fresenius\u2019 J. Anal. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1021\/cr030183i","article-title":"Functional nucleic acid sensors","volume":"109","author":"Liu","year":"2009","journal-title":"Chem. Rev."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"9838","DOI":"10.1021\/ja0717358","article-title":"A DNAzyme catalytic beacon sensor for paramagnetic Cu2+ ions in aqueous solution with high sensitivity and selectivity","volume":"129","author":"Liu","year":"2007","journal-title":"J. Am. Chem. Soc."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2056","DOI":"10.1073\/pnas.0607875104","article-title":"A catalytic beacon sensor for uranium with parts-per-trillion sensitivity and millionfold selectivity","volume":"104","author":"Liu","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4346","DOI":"10.1002\/anie.200800960","article-title":"A highly selective DNAzyme sensor for mercuric ions","volume":"47","author":"Hollenstein","year":"2008","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3896","DOI":"10.1039\/b926910j","article-title":"A highly selective lead sensor based on a classic lead DNAzyme","volume":"46","author":"Lan","year":"2010","journal-title":"Chem. Commun."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.jcis.2015.11.073","article-title":"In situ regulation nanoarchitecture of Au nanoparticles\/reduced graphene oxide colloid for sensitive and selective SERS detection of lead ions","volume":"465","author":"Zhao","year":"2016","journal-title":"J. Colloid Interface Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5005","DOI":"10.1021\/ac1009047","article-title":"Catalytic and Molecular Beacons for Amplified Detection of Metal Ions and Organic Molecules with High Sensitivity","volume":"82","author":"Zhang","year":"2010","journal-title":"Anal. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2125","DOI":"10.1016\/j.bios.2010.09.018","article-title":"Improving Pb2+ detection using DNAzyme-based fluorescence sensors by pairing fluorescence donors with gold nanoparticles","volume":"26","author":"Kim","year":"2011","journal-title":"Biosens. Bioelectron."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1021\/ja067278x","article-title":"Electrochemical detection of parts-per-billion lead via an electrode-bound DNAzyme assembly","volume":"129","author":"Xiao","year":"2007","journal-title":"J. Am. Chem. Soc."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1039\/b718624j","article-title":"Immobilization of DNAzyme catalytic beacons on PMMA for Pb 2+ detection","volume":"8","author":"Dalavoy","year":"2008","journal-title":"Lab Chip"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1093\/nar\/gkl1056","article-title":"Efficient signaling platforms built from a small catalytic DNA and doubly labeled fluorogenic substrates","volume":"35","author":"Chiuman","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1021\/ar300011g","article-title":"\u201cFitting\u201d Makes \u201cSensing\u201d Simple: Label-Free Detection Strategies Based on Nucleic Acid Aptamers","volume":"46","author":"Du","year":"2013","journal-title":"Accounts Chem. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"90300","DOI":"10.1039\/C6RA15750E","article-title":"Highly sensitive and selective optical detection of lead(II) using a label-free fluorescent aptasensor","volume":"6","author":"Huang","year":"2016","journal-title":"RSC Adv."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.1021\/ac902638v","article-title":"Lead(II)-induced allosteric G-quadruplex DNAzyme as a colorimetric and chemiluminescence sensor for highly sensitive and selective Pb2+ detection","volume":"82","author":"Li","year":"2010","journal-title":"Anal. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1021\/ac1028787","article-title":"Fluorescence detection of Lead(II) ions through their induced catalytic activity of DNAzymes","volume":"83","author":"Li","year":"2011","journal-title":"Anal. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.talanta.2015.10.004","article-title":"A label-free DNAzyme fluorescence biosensor for amplified detection of Pb2+-based on cleavage-induced G-quadruplex formation","volume":"147","author":"Fu","year":"2016","journal-title":"Talanta"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.aca.2014.01.053","article-title":"Label-free fluorescent biosensor based on the target recycling and Thioflavin T-induced quadruplex formation for short DNA species of c-erbB-2 detection","volume":"817","author":"Chen","year":"2014","journal-title":"Anal. Chim. Acta"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1021\/ja309588h","article-title":"Thioflavin T as an Efficient Inducer and Selective Fluorescent Sensor for the Human Telomeric G-Quadruplex DNA","volume":"135","author":"Mohanty","year":"2013","journal-title":"J. Am. Chem. Soc."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"5620","DOI":"10.1021\/bi4006072","article-title":"Multiple and cooperative binding of fluorescence light-up probe thioflavin t with human telomere DNA G-Quadruplex","volume":"52","author":"Gabelica","year":"2013","journal-title":"Biochemistry"},{"key":"ref_32","unstructured":"Faverie, A.R., Gu\u00e9din, A., Bedrat, A., Yatsunyk, L.A., and Mergny, J.-L. (2014). Thioflavin T as a fluorescence light-up probe for G4 formation. Nucleic Acids Res."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/16\/12\/2155\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T19:28:41Z","timestamp":1760210921000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/16\/12\/2155"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12,16]]},"references-count":32,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2016,12]]}},"alternative-id":["s16122155"],"URL":"https:\/\/doi.org\/10.3390\/s16122155","relation":{},"ISSN":["1424-8220"],"issn-type":[{"type":"electronic","value":"1424-8220"}],"subject":[],"published":{"date-parts":[[2016,12,16]]}}}