{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T13:56:05Z","timestamp":1768485365091,"version":"3.49.0"},"reference-count":20,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2016,12,30]],"date-time":"2016-12-30T00:00:00Z","timestamp":1483056000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Natural Science Foundation","award":["61527815, 61501426, 31500800, 61601435"],"award-info":[{"award-number":["61527815, 61501426, 31500800, 61601435"]}]},{"name":"the Key Programs of the Chinese Academy of Sciences","award":["KJZD-EW-L11\u20132"],"award-info":[{"award-number":["KJZD-EW-L11\u20132"]}]},{"name":"the Beijing Science and Technology Plan","award":["Z141100000214002"],"award-info":[{"award-number":["Z141100000214002"]}]},{"name":"Open Project of Beijing Key Laboratory on Research of Parkinson's Disease"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Changes in the structure and function of the hippocampus contribute to epilepsy, schizophrenia and other neurological or mental disorders of the brain. Since the function of the hippocampus depends heavily on the glutamate (Glu) signaling pathways, in situ real-time detection of Glu neurotransmitter release and electrophysiological signals in hippocampus is of great significance. To achieve the dual-mode detection in mouse hippocampus in vivo, a 16-channel implantable microelectrode array (MEA) was fabricated by micro-electromechanical system (MEMS) technology. Twelve microelectrode sites were modified with platinum black for electrophysiological recording and four sites were modified with glutamate oxidase (GluOx) and 1,3-phenylenediamine (mPD) for selective electrochemical detection of Glu. The MEA was implanted from cortex to hippocampus in mouse brain for in situ real-time monitoring of Glu and electrophysiological signals. It was found that the Glu concentration in hippocampus was roughly 50 \u03bcM higher than that in the cortex, and the firing rate of concurrently recorded spikes declined from 6.32 \u00b1 4.35 spikes\/s in cortex to 0.09 \u00b1 0.06 spikes\/s in hippocampus. The present results demonstrated that the dual-mode MEA probe was capable in neurological detections in vivo with high spatial resolution and dynamical response, which lays the foundation for further pathology studies in the hippocampus of mouse models with nervous or mental disorders.<\/jats:p>","DOI":"10.3390\/s17010061","type":"journal-article","created":{"date-parts":[[2016,12,30]],"date-time":"2016-12-30T08:48:53Z","timestamp":1483087733000},"page":"61","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["In Situ Real-Time Monitoring of Glutamate and Electrophysiology from Cortex to Hippocampus in Mice Based on a Microelectrode Array"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7421-5747","authenticated-orcid":false,"given":"Xinyi","family":"Fan","sequence":"first","affiliation":[{"name":"State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100190, China"}]},{"given":"Yilin","family":"Song","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100190, China"}]},{"given":"Yuanlin","family":"Ma","sequence":"additional","affiliation":[{"name":"Institute of Mental Health, the Sixth Hospital of Peking University, Beijing 100191, China"}]},{"given":"Song","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100190, China"}]},{"given":"Guihua","family":"Xiao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100190, China"}]},{"given":"Lili","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100190, China"}]},{"given":"Huiren","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100190, China"}]},{"given":"Dai","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Mental Health, the Sixth Hospital of Peking University, Beijing 100191, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5997-7252","authenticated-orcid":false,"given":"Xinxia","family":"Cai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100190, China"}]}],"member":"1968","published-online":{"date-parts":[[2016,12,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1124\/jpet.107.131698","article-title":"Second-by-second measures of L-glutamate in the prefrontal cortex and striatum of freely moving mice","volume":"324","author":"Hascup","year":"2008","journal-title":"J. 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