{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T01:05:31Z","timestamp":1771031131859,"version":"3.50.1"},"reference-count":33,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2017,1,27]],"date-time":"2017-01-27T00:00:00Z","timestamp":1485475200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["TRR60"],"award-info":[{"award-number":["TRR60"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["TRR130"],"award-info":[{"award-number":["TRR130"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Deutsche Forschungsgemeinschaft within the Collaborative Research Center (SFB)","award":["SFB876 project B2"],"award-info":[{"award-number":["SFB876 project B2"]}]},{"name":"Deutsche Forschungsgemeinschaft within the Collaborative Research Center (SFB)","award":["SFB876 project C1"],"award-info":[{"award-number":["SFB876 project C1"]}]},{"name":"FP7 EC Project &quot;Nanodetector&quot;","award":["FP7-NMP-2011-SME-5,#280478"],"award-info":[{"award-number":["FP7-NMP-2011-SME-5,#280478"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The PAMONO-sensor (plasmon assisted microscopy of nano-objects) demonstrated an ability to detect and quantify individual viruses and virus-like particles. However, another group of biological vesicles\u2014microvesicles (100\u20131000 nm)\u2014also attracts growing interest as biomarkers of different pathologies and needs development of novel techniques for characterization. This work shows the applicability of a PAMONO-sensor for selective detection of microvesicles in aquatic samples. The sensor permits comparison of relative concentrations of microvesicles between samples. We also study a possibility of repeated use of a sensor chip after elution of the microvesicle capturing layer. Moreover, we improve the detection features of the PAMONO-sensor. The detection process utilizes novel machine learning techniques on the sensor image data to estimate particle size distributions of nano-particles in polydisperse samples. Altogether, our \ufb01ndings expand analytical features and the application \ufb01eld of the PAMONO-sensor. They can also serve for a maturation of diagnostic tools based on the PAMONO-sensor platform.<\/jats:p>","DOI":"10.3390\/s17020244","type":"journal-article","created":{"date-parts":[[2017,1,27]],"date-time":"2017-01-27T11:23:02Z","timestamp":1485516182000},"page":"244","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Application of the PAMONO-Sensor for Quanti\ufb01cation of Microvesicles and Determination of Nano-Particle Size Distribution"],"prefix":"10.3390","volume":"17","author":[{"given":"Victoria","family":"Shpacovitch","sequence":"first","affiliation":[{"name":"Leibniz Institute f\u00fcr Analytische Wissenschaften, ISAS e.V., Bunsen-Kirchhoff-Stra\u00dfe 11, 44139 Dortmund, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina","family":"Sidorenko","sequence":"additional","affiliation":[{"name":"MIVITEC GmbH, Wamslerstra\u00dfe.4, 81829 Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Lenssen","sequence":"additional","affiliation":[{"name":"Department of Computer Science VII, TU Dortmund University, Otto-Hahn-Stra\u00dfe. 16, 44227Dortmund,Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Temchura","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Molecular Virology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-N\u00fcrnberg, Schlossgarten 4, 91054 Erlangen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2530-8197","authenticated-orcid":false,"given":"Frank","family":"Weichert","sequence":"additional","affiliation":[{"name":"Department of Computer Science VII, TU Dortmund University, Otto-Hahn-Stra\u00dfe. 16, 44227Dortmund,Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heinrich","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Computer Science VII, TU Dortmund University, Otto-Hahn-Stra\u00dfe. 16, 44227Dortmund,Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"\u00dcberla","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Molecular Virology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-N\u00fcrnberg, Schlossgarten 4, 91054 Erlangen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Zybin","sequence":"additional","affiliation":[{"name":"Leibniz Institute f\u00fcr Analytische Wissenschaften, ISAS e.V., Bunsen-Kirchhoff-Stra\u00dfe 11, 44139 Dortmund, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7670-7529","authenticated-orcid":false,"given":"Alexander","family":"Schramm","sequence":"additional","affiliation":[{"name":"Children\u2019s Hospital, Oncology Laboratory, University Clinic Essen, Hufelandstra\u00dfe. 55, 45122 Essen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland","family":"Hergenr\u00f6der","sequence":"additional","affiliation":[{"name":"Leibniz Institute f\u00fcr Analytische Wissenschaften, ISAS e.V., Bunsen-Kirchhoff-Stra\u00dfe 11, 44139 Dortmund, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,1,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s11468-009-9111-5","article-title":"Real-time Detection of Single Immobilized Nanoparticles by Surface Plasmon Resonance Imaging","volume":"5","author":"Zybin","year":"2010","journal-title":"Plasmonics"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1016\/j.snb.2011.09.050","article-title":"Analytical features of particle counting sensor based on plasmon assisted microscopy of nano objects","volume":"160","author":"Gurevich","year":"2011","journal-title":"Sens. Actuators B Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"10481","DOI":"10.3390\/s150510481","article-title":"Surface Plasmon Resonance: A Versatile Technique for Biosensor Applications","volume":"15","author":"Nguyen","year":"2015","journal-title":"Sensors"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1080\/02726340801921650","article-title":"Surface Plasmon Resonance for Biosensing: A Mini-Review","volume":"28","author":"Abdulhalim","year":"2008","journal-title":"Electromagnetics"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"4173","DOI":"10.1039\/C5AY00277J","article-title":"A peptide nucleic acid label-free biosensor for Mycobacterium tuberculosis DNA detection via azimuthally controlled grating-coupled SPR","volume":"7","author":"Silvestry","year":"2015","journal-title":"Anal. Methods"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1039\/C6LC00057F","article-title":"A surface acoustic wave (SAW)-enhanced grating-coupling phase-interrogation surface plasmon resonance (SPR) microfluidic biosensor","volume":"16","author":"Sonato","year":"2016","journal-title":"Lab Chip"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"16028","DOI":"10.1073\/pnas.1005264107","article-title":"Label-free imaging, detection, and mass measurement of single viruses by surface plasmon resonance","volume":"107","author":"Wang","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1021\/nn405868e","article-title":"Single-Nanoparticle Near-Infrared Surface Plasmon Resonance Microscopy for Real-Time Measurements of DNA Hybridization Adsorption","volume":"8","author":"Halpern","year":"2014","journal-title":"ACS Nano"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.ab.2015.06.022","article-title":"Application of surface plasmon resonance imaging technique for the detection of single spherical biological submicrometer particles","volume":"486","author":"Shpacovitch","year":"2015","journal-title":"Anal. Biochem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"U490","DOI":"10.1038\/nbt.2886","article-title":"Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor","volume":"32","author":"Im","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"8857","DOI":"10.1021\/ac5023056","article-title":"Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging","volume":"86","author":"Zhu","year":"2014","journal-title":"Anal. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Yanez-Mo, M., Siljander, P.R., Andreu, Z., Zavec, A.B., Borras, F.E., Buzas, E.I., Buzas, K., Casal, E., Cappello, F., and Carvalho, J. (2015). Biological properties of extracellular vesicles and their physiological functions. J. Extracell. Vesicles, 4.","DOI":"10.3402\/jev.v4.27066"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2667","DOI":"10.1007\/s00018-011-0689-3","article-title":"Membrane vesicles, current state-of-the-art: emerging role of extracellular vesicles","volume":"68","author":"Gyorgy","year":"2011","journal-title":"Cell Mol. Life Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1002\/cyto.a.22795","article-title":"Analytical challenges of extracellular vesicle detection: A comparison of different techniques","volume":"89","author":"Erdbrugger","year":"2016","journal-title":"Cytometry A"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1007\/BF01395428","article-title":"Determination of Optical Constants of Metals by Excitation of Surface Plasmons","volume":"241","author":"Kretschmann","year":"1971","journal-title":"Z. Phys."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.snb.2016.07.124","article-title":"Optimal conditions for SPR-imaging of nano-objects","volume":"239","author":"Zybin","year":"2017","journal-title":"Sens. Actuators B Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1007\/s00604-015-1599-0","article-title":"Computer assisted detection and quantification of single adsorbing nanoparticles by differential surface plasmon microscopy","volume":"183","author":"Sidorenko","year":"2016","journal-title":"Microchim. Acta"},{"key":"ref_18","unstructured":"Siedhoff, D., Libuschewski, P., Weichert, F., Zybin, A., Marwedel, P., and M\u00fcller, H. (2014). Bildverarbeitung f\u00fcr die Medizin. Lecture Notes in Informatics, Springer. (In Germany)."},{"key":"ref_19","unstructured":"Libuschewski, P., Siedhoff, D., Timm, C., Gelenberg, A., and Weichert, F. (2013, January 11\u201314). Fuzzy-enhanced real-time capable detection of biological viruses using a portable biosensor. Proceedings of the International Joint Conference on Biomedical Engineering Systems and Technologies (Biosignals), Barcelona, Spain."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5088","DOI":"10.1016\/j.vaccine.2013.08.055","article-title":"HIV-derived lentiviral particles promote T-cell independent activation and differentiation of naive cognate conventional B2-cells in vitro","volume":"31","author":"Gardt","year":"2013","journal-title":"Vaccine"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2403","DOI":"10.1089\/104303400750038507","article-title":"Rev-independent expression of synthetic gag-pol genes of human immunodeficiency virus type 1 and simian immunodeficiency virus: Implications for the safety of lentiviral vectors","volume":"11","author":"Wagner","year":"2000","journal-title":"Hum. Gene Ther."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3662","DOI":"10.1016\/j.vaccine.2008.04.069","article-title":"Enhancement of immunostimulatory properties of exosomal vaccines by incorporation of fusion-competent G protein of vesicular stomatitis virus","volume":"26","author":"Temchura","year":"2008","journal-title":"Vaccine"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0168-1702(02)00316-7","article-title":"Budding and secretion of HIV Gag-Env virus-like particles from recombinant human adenovirus infected cells","volume":"92","author":"Luo","year":"2003","journal-title":"Virus Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.ymeth.2006.07.018","article-title":"Virus-like particles: Passport to immune recognition","volume":"40","author":"Grgacic","year":"2006","journal-title":"Methods"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"7902","DOI":"10.1038\/sj.onc.1208936","article-title":"Prediction of clinical outcome and biological characterization of neuroblastoma by expression profiling","volume":"24","author":"Schramm","year":"2005","journal-title":"Oncogene"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/0893-6080(91)90009-T","article-title":"Approximation Capabilities of Multilayer Feedforward Networks","volume":"4","author":"Hornik","year":"1991","journal-title":"Neural Netw."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1037\/h0042519","article-title":"The Perceptron: A Probabilistic Model for Information Storage and Organization in the Brain","volume":"65","author":"Rosenblatt","year":"1958","journal-title":"Psychol. Rev."},{"key":"ref_28","unstructured":"Pereira, F., Burges, C.J.C., Bottou, L., and Weinberger, K.Q. (2012). Advances in Neural Information Processing Systems 25, Curran Associates, Inc."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.1109\/TPAMI.2012.231","article-title":"Learning Hierarchical Features for Scene Labeling","volume":"35","author":"Farabet","year":"2013","journal-title":"IEEE Trans. Pattern Anal. Mach. Intell."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2278","DOI":"10.1109\/5.726791","article-title":"Gradient-based learning applied to document recognition","volume":"86","author":"Lecun","year":"1998","journal-title":"Proc. IEEE"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1038\/323533a0","article-title":"Learning representations by back-propagating errors","volume":"323","author":"Rumelhart","year":"1986","journal-title":"Nature"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2680","DOI":"10.1021\/ac0619231","article-title":"Direct immobilization of protein G variants with various numbers of cysteine residues on a gold surface","volume":"79","author":"Lee","year":"2007","journal-title":"Anal. Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.nbd.2011.01.029","article-title":"Lysosomal dysfunction increases exosome-mediated alpha-synuclein release and transmission","volume":"42","author":"Seow","year":"2011","journal-title":"Neurobiol. Dis."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/17\/2\/244\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:27:09Z","timestamp":1760207229000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/17\/2\/244"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1,27]]},"references-count":33,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2017,2]]}},"alternative-id":["s17020244"],"URL":"https:\/\/doi.org\/10.3390\/s17020244","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1,27]]}}}