{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T03:22:56Z","timestamp":1762917776391,"version":"build-2065373602"},"reference-count":20,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2017,4,2]],"date-time":"2017-04-02T00:00:00Z","timestamp":1491091200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Escherichia coli O157:H7 is known to cause serious diseases including hemorrhagic colitis and hemolytic uremic syndrome. A gold nanoparticle lateral flow immunoassay (Au-LFIA) was used to detect Escherichia coli O157:H7 in ground pork samples. False-positive results were detected using Au-LFIA; a Citrobacter freundii strain was isolated from the ground pork samples and identified by using CHROmagarTM plates, API 20E, and 16S RNA sequencing. Since C. freundii showed cross-reactivity with E. coli O157:H7 when Au-LFIA test strips were used, a novel method combining modified enrichment with a lateral flow immunoassay for accurate and convenient detection of E. coli O157:H7 in ground pork was developed in this study to minimize these false positives. MacConkey broth was optimized for E. coli O157:H7 enrichment and C. freundii inhibition by the addition of 5 mg\/L potassium tellurite and 0.10 mg\/L cefixime. Using the proposed modified enrichment procedure, the false-positive rate of ground pork samples spiked with 100 CFU\/g C. freundii decreased to 5%.<\/jats:p>","DOI":"10.3390\/s17040753","type":"journal-article","created":{"date-parts":[[2017,4,3]],"date-time":"2017-04-03T13:32:30Z","timestamp":1491226350000},"page":"753","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Strategy for Accurate Detection of Escherichia coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay"],"prefix":"10.3390","volume":"17","author":[{"given":"Song","family":"Cheng","sequence":"first","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China"}]},{"given":"Ming-hui","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China"}]},{"given":"Gang-gang","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China"}]},{"given":"Zhi-biao","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Science and Technology, Nanchang University, Nanchang 330047, China"}]},{"given":"Dao-feng","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China"}]},{"given":"Yong-hua","family":"Xiong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China"}]},{"given":"Hua","family":"Wei","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China"}]},{"given":"Wei-hua","family":"Lai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China"}]}],"member":"1968","published-online":{"date-parts":[[2017,4,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1428","DOI":"10.1016\/S0140-6736(10)60963-4","article-title":"Escherichia coli O157","volume":"376","author":"Pennington","year":"2010","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.ijfoodmicro.2013.10.026","article-title":"Detection of viable Escherichia coli O157:H7 in ground beef by propidium monoazide real-time PCR","volume":"170","author":"Liu","year":"2014","journal-title":"Int. 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