{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:41:56Z","timestamp":1761896516562,"version":"build-2065373602"},"reference-count":33,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2017,4,11]],"date-time":"2017-04-11T00:00:00Z","timestamp":1491868800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Colorectal cancer (CRC) is an aggressive human malignancy with a complex genomic landscape harboring KRAS mutations. In 40%\u201360% of patients with CRC, constantly active KRAS proteins affect the prognosis, surgical strategy, and clinical benefit from therapy with anti-epidermal growth factor receptor (EGFR) agents. For this reason, there is a greater demand for minimally-invasive diagnostic devices to characterize the genetic pattern and prevent the acquired mechanism to drug resistance. The rapid developments in cutting-edge diagnostic techniques are expected to play a growing role in medicine and represent an attractive promise to identify potential responders to personalized medicine. Here we propose a new method to simultaneously detect the main KRAS mutations on the portable real-time PCR Q3 platform. This platform is based on hybrid silicon-plastic technology implemented in a miniaturized chip able to achieve a sample-in answer-out rapid analysis, allowing a new approach to genetic counseling and testing.<\/jats:p>","DOI":"10.3390\/s17040831","type":"journal-article","created":{"date-parts":[[2017,4,11]],"date-time":"2017-04-11T11:41:42Z","timestamp":1491910902000},"page":"831","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Miniaturized Real-Time PCR on a Q3 System for Rapid KRAS Genotyping"],"prefix":"10.3390","volume":"17","author":[{"given":"Maria","family":"Guarnaccia","sequence":"first","affiliation":[{"name":"Institute of Neurological Sciences, Italian National Research Council, Via Paolo Gaifami, 18-95125 Catania, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9297-8115","authenticated-orcid":false,"given":"Rosario","family":"Iemmolo","sequence":"additional","affiliation":[{"name":"Institute of Neurological Sciences, Italian National Research Council, Via Paolo Gaifami, 18-95125 Catania, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salvatore","family":"Petralia","sequence":"additional","affiliation":[{"name":"STMicroelectronics, Stradale Primosole, 50-95121 Catania, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5874-7284","authenticated-orcid":false,"given":"Sabrina","family":"Conoci","sequence":"additional","affiliation":[{"name":"STMicroelectronics, Stradale Primosole, 50-95121 Catania, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7590-1792","authenticated-orcid":false,"given":"Sebastiano","family":"Cavallaro","sequence":"additional","affiliation":[{"name":"Institute of Neurological Sciences, Italian National Research Council, Via Paolo Gaifami, 18-95125 Catania, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,4,11]]},"reference":[{"key":"ref_1","first-page":"5171","article-title":"Kras mutation testing in metastatic colorectal cancer","volume":"18","author":"Tan","year":"2012","journal-title":"World J. 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