{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T16:18:32Z","timestamp":1774801112911,"version":"3.50.1"},"reference-count":43,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2017,5,9]],"date-time":"2017-05-09T00:00:00Z","timestamp":1494288000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this work, a novel nanocomposite consisting of electrosynthesized gold nanodendrites and chitosan nanoparticles (AuNDs\/CSNPs) has been prepared to fabricate an impedimetric immunosensor based on a screen printed carbon electrode (SPCE) for the rapid and sensitive immunoassay of botulinum neurotoxin A (BoNT\/A). BoNT\/A polyclonal antibody was immobilized on the nanocomposite-modified SPCE for the signal amplification. The structure of the prepared nanocomposite was investigated by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The charge transfer resistance (RCT) changes were used to detect BoNT\/A as the specific immuno-interactions at the immunosensor surface that efficiently limited the electron transfer of Fe(CN)63\u2212\/4\u2212 as a redox probe at pH = 7.4. A linear relationship was observed between the %\u2206RCT and the concentration logarithm of BoNT\/A within the range of 0.2 to 230 pg\u00b7mL\u22121 with a detection limit (S\/N = 3) of 0.15 pg\u00b7mL\u22121. The practical applicability of the proposed sensor was examined by evaluating the detection of BoNT\/A in milk and serum samples with satisfactory recoveries. Therefore, the prepared immunosensor holds great promise for the fast, simple and sensitive detection of BoNT\/A in various real samples.<\/jats:p>","DOI":"10.3390\/s17051074","type":"journal-article","created":{"date-parts":[[2017,5,9]],"date-time":"2017-05-09T11:50:48Z","timestamp":1494330648000},"page":"1074","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Fabrication of a Novel Highly Sensitive and Selective Immunosensor for Botulinum Neurotoxin Serotype A Based on an Effective Platform of Electrosynthesized Gold Nanodendrites\/Chitosan Nanoparticles"],"prefix":"10.3390","volume":"17","author":[{"given":"Rahim","family":"Sorouri","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran 1435116471, Iran"}]},{"given":"Hasan","family":"Bagheri","sequence":"additional","affiliation":[{"name":"Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran 1435116471, Iran"}]},{"given":"Abbas","family":"Afkhami","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Bu-Ali Sina University, Hamedan 6517838695, Iran"}]},{"given":"Jafar","family":"Salimian","sequence":"additional","affiliation":[{"name":"Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran 1435116471, Iran"}]}],"member":"1968","published-online":{"date-parts":[[2017,5,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1021\/ac504054u","article-title":"Centrifugal microfluidic platform for ultrasensitive detection of botulinum toxin","volume":"87","author":"Koh","year":"2015","journal-title":"Anal. 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