{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T15:06:43Z","timestamp":1770563203611,"version":"3.49.0"},"reference-count":29,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A wavelength-interrogated surface plasmon resonance (SPR) sensor based on a nanoporous gold (NPG) film has been fabricated for the sensitive detection of trace quantities of benzo[a]pyrene (BaP) in water. The large-area uniform NPG film was prepared by a two-step process that includes sputtering deposition of a 60-nm-thick AuAg alloy film on a glass substrate and chemical dealloying of the alloy film in nitric acid. For SPR sensor applications, the NPG film plays the dual roles of analyte enrichment and supporting surface plasmon waves, which leads to sensitivity enhancement. In this work, the as-prepared NPG film was first modified with 1-dodecanethiol molecules to make the film hydrophobic so as to improve BaP enrichment from water via hydrophobic interactions. The SPR sensor with the hydrophobic NPG film enables one to detect BaP at concentrations as low as 1 nmol\u00b7L\u22121. In response to this concentration of BaP the sensor produced a resonance-wavelength shift of \u0394\u03bbR = 2.22 nm. After the NPG film was functionalized with mouse monoclonal IgG1 that is the antibody against BaP, the sensor\u2019s sensitivity was further improved and the BaP detection limit decreased further down to 5 pmol\u00b7L\u22121 (the corresponding \u0394\u03bbR = 1.77 nm). In contrast, the conventional SPR sensor with an antibody-functionalized dense gold film can give a response of merely \u0394\u03bbR = 0.9 nm for 100 pmol\u00b7L\u22121 BaP.<\/jats:p>","DOI":"10.3390\/s17061255","type":"journal-article","created":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T10:36:36Z","timestamp":1496313396000},"page":"1255","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Nanoporous Gold Films Prepared by a Combination of Sputtering and Dealloying for Trace Detection of Benzo[a]pyrene Based on Surface Plasmon Resonance Spectroscopy"],"prefix":"10.3390","volume":"17","author":[{"given":"Li","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China"},{"name":"School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiu-Mei","family":"Wan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China"},{"name":"School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ran","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan-Feng","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi-Mei","family":"Qi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1021\/ac0257546","article-title":"Detection of Polycyclic Aromatic Hydrocarbons Using Quartz Crystal Microbalances","volume":"75","author":"Stanley","year":"2003","journal-title":"Anal. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1039\/b609875d","article-title":"Excitation-emission matrix fluorescence spectroscopy in conjunction with multiway analysis for PAH detection in complex matrices","volume":"131","author":"Nahorniak","year":"2006","journal-title":"Analyst"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"17829","DOI":"10.1021\/jp203181c","article-title":"Preparation of Thiol Modified Fe3O4@Ag Magnetic SERS Probe for PAHs Detection and Identification","volume":"115","author":"Du","year":"2011","journal-title":"J. Phys. Chem. C"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3736","DOI":"10.1016\/S1452-3981(23)08046-X","article-title":"Detecting of Benzo[a]pyrene Using a Label-free Amperometric Immunosensor","volume":"9","author":"Zhang","year":"2014","journal-title":"Int. J. Electrochem. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.snb.2015.01.134","article-title":"Functionalized Au nanoparticles for label-free Raman determination of ppb level benzopyrene in edible oil","volume":"212","author":"Fu","year":"2015","journal-title":"Sens. Actuators B Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.jhazmat.2012.02.038","article-title":"Use of intestinal Pseudomonas aeruginosa in fish to detect the environmental pollutant benzo[a]pyrene","volume":"215","author":"Karami","year":"2012","journal-title":"J. Hazard. Mater."},{"key":"ref_7","unstructured":"(2017, May 08). National Primary Drinking Water Regulations, Available online: https:\/\/www.epa.gov\/ground-water-and-drinking-water\/national-primary-drinking-water-regulations."},{"key":"ref_8","unstructured":"(2017, May 27). Standards for Drinking Water Quality, Available online: http:\/\/www.nhfpc.gov.cn\/zhuz\/pgw\/201212\/33644.shtml."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.msec.2016.08.040","article-title":"Rapid, efficient and selective preconcentration of benzo[a]pyrene (BaP) by molecularly imprinted composite cartridge and HPLC","volume":"70","author":"Armutcu","year":"2017","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1007\/s10337-013-2575-7","article-title":"Detection of Benzo[a]pyrene in Fried Food by Ultrasound-Assisted Matrix Solid-Phase Dispersion and Isotope Dilution GC\u2013MS","volume":"76","author":"Liu","year":"2013","journal-title":"Chromatographia"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4010","DOI":"10.1039\/c2an35589b","article-title":"Surface enhanced Raman spectroscopic detection of polycyclic aromatic hydrocarbons (PAHs) using a gold nanoparticles-modified alginate gel network","volume":"137","author":"Bao","year":"2012","journal-title":"Analyst"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aca.2003.11.002","article-title":"Highly sensitive and selective fluorescence optosensor to detect and quantify benzo[a]pyrene in water samples","volume":"506","year":"2004","journal-title":"Anal. Chim. Acta"},{"key":"ref_13","unstructured":"(2017, May 27). ChemSpider Search and Share Chemistry, Benzo(a)pyrene. Available online: http:\/\/www.chemspider.com\/Chemical-Structure.2246.html."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2485","DOI":"10.1364\/OE.24.002485","article-title":"Highly sensitive multi-core flat fiber surface plasmon resonance refractive index sensor","volume":"24","author":"Rifat","year":"2016","journal-title":"Opt. Exp."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1951","DOI":"10.1364\/OL.38.001951","article-title":"Surface plasmon resonance optical cavity enhanced refractive index sensing","volume":"38","author":"Giorgini","year":"2013","journal-title":"Opt. Lett."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.3390\/s9031339","article-title":"Toxin Detection by Surface Plasmon Resonance","volume":"9","author":"Hodnik","year":"2009","journal-title":"Sensors"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3342","DOI":"10.1021\/jp2102429","article-title":"Application of Porous TiO2 Thin Films as Wavelength-Interrogated Waveguide Resonance Sensors for Bio\/Chemical Detection","volume":"116","author":"Zhang","year":"2012","journal-title":"J. Phys. Chem. C"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1016\/j.snb.2014.07.033","article-title":"Hierarchical mesoporous silica film modified near infrared SPR sensorwith high sensitivities to small and large molecules","volume":"203","author":"Li","year":"2014","journal-title":"Sens. Actuators B Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1016\/j.snb.2005.07.060","article-title":"Enhancement of surface plasmon resonance (SPR) signals using organic functionalized mesoporous silica on a gold film","volume":"114","author":"Oh","year":"2006","journal-title":"Sens. Actuators B Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"6066","DOI":"10.1021\/ac100654b","article-title":"Properties of A Metal Clad Waveguide Sensor Based on A Nanoporous-Metal-Oxide\/Metal Multilayer Film","volume":"82","author":"Hotta","year":"2010","journal-title":"Anal. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"867","DOI":"10.3866\/PKU.WHXB201302222","article-title":"Surface Plasmon Resonance Sensing Properties of Nanoporous Gold Thin Films","volume":"29","author":"Zhang","year":"2013","journal-title":"Acta Phys. Chim. Sin."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1186\/s11671-016-1327-7","article-title":"Surface Plasmon\u2019s Dispersion Properties of Porous Gold Films","volume":"11","author":"Stetsenko","year":"2016","journal-title":"Nanoscale Res. Lett."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"093701(1-4)","DOI":"10.1063\/1.3560482","article-title":"Localized surface plasmon resonance of nanoporous gold","volume":"98","author":"Lang","year":"2011","journal-title":"Appl. Phys. Lett."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3949","DOI":"10.1021\/la903293b","article-title":"Self-Assembled Monolayers of Aromatic \u03c9-Aminothiols on Gold: Surface Chemistry and Reactivity","volume":"26","author":"Dietrich","year":"2010","journal-title":"Langmuir"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.aca.2016.02.009","article-title":"Au nanoparticles grafted on Fe3O4 as effective SERS substrates for label-free detection of the 16 EPA priority polycyclic aromatic hydrocarbons","volume":"915","author":"Du","year":"2016","journal-title":"Anal. Chim. Acta"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1016\/j.snb.2004.11.069","article-title":"Compact surface plasmon resonance (SPR) immunosensor using multichannel for simultaneous detection of small molecule compounds","volume":"108","author":"Kawazumi","year":"2005","journal-title":"Sens. Actuators B Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3761","DOI":"10.1016\/j.bios.2011.02.028","article-title":"Sensitive immunosensor for benzo[a]pyrene detection based on dual amplification strategy of PAMAM dendrimer and amino-modified methylene blue\/SiO2 core-shell nanoparticles","volume":"26","author":"Lin","year":"2011","journal-title":"Biosens. Bioelectron."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4538","DOI":"10.1021\/acs.analchem.5b00648","article-title":"Detection of the Carcinogenic Water Pollutant Benzo[a]pyrene with an Electro-Switchable Biosurface","volume":"87","author":"Lux","year":"2015","journal-title":"Anal. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0003-2670(99)00030-6","article-title":"Flow injection immunosensing of polycyclic aromatic hydrocarbons with a quartz crystal microbalance","volume":"387","author":"Liu","year":"1999","journal-title":"Anal. Chim. Acta"}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/17\/6\/1255\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:37:34Z","timestamp":1760207854000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/17\/6\/1255"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6,1]]},"references-count":29,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2017,6]]}},"alternative-id":["s17061255"],"URL":"https:\/\/doi.org\/10.3390\/s17061255","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6,1]]}}}