{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T15:37:29Z","timestamp":1764603449204,"version":"build-2065373602"},"reference-count":20,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2017,6,19]],"date-time":"2017-06-19T00:00:00Z","timestamp":1497830400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81371691","31571005","81501617"],"award-info":[{"award-number":["81371691","31571005","81501617"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["106112016CDJZR238807"],"award-info":[{"award-number":["106112016CDJZR238807"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Program of International S&amp;T Cooperation","award":["2014DFG31380"],"award-info":[{"award-number":["2014DFG31380"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A portable multichannel surface plasmon resonance (SPR) biosensor device is presented in this study. As an optical biosensor device, the core component of its light path is a semi-cylindrical prism, which is used as the coupling unit for the excitation of the SPR phenomena. Based on this prism, a wedge-shaped incident light beam including a continuous angle range (10\u00b0) is chosen to replace the commonly-used parallel light beam in traditional SPR devices, in which the incident angle is adjusted by a sophisticated mechanical system. Thus, complicated, cumbersome, and costly mechanical structures can be avoided in this design. Furthermore, the selection of a small and high-stability semiconductor laser and matrix CCD detector as well as a microfluidic system aids in the realization of a miniaturized and multichannel device. Several different samples were used to test the performance of this new device. For ethanol with different concentrations, the sensing response was of good linear relativity with the concentration (Y = 3.17143X + 2.81518, R2 = 0.97661). Mouse IgG and goat anti-mouse IgG were used as biological samples for immunological analysis, and BSA as the control group. Good specific recognition between mouse IgG and goat anti-mouse IgG has been achieved. The detection limit of antibody to antigen coated on the sensing surface was about 25 mg\/L without surface modification.<\/jats:p>","DOI":"10.3390\/s17061435","type":"journal-article","created":{"date-parts":[[2017,6,19]],"date-time":"2017-06-19T10:29:26Z","timestamp":1497868166000},"page":"1435","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Design and Performance of a Portable and Multichannel SPR Device"],"prefix":"10.3390","volume":"17","author":[{"given":"Xiao-ling","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Biorheological Science and Technology, Chongqing University, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China"}]},{"given":"Yan","family":"Liu","sequence":"additional","affiliation":[{"name":"Chongqing Engineering Research Center of Medical Electronics Technology, Chongqing University, Bioengineering College, Chongqing University, Chongqing 400030, China"}]},{"given":"Ting","family":"Fan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biorheological Science and Technology, Chongqing University, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China"},{"name":"Chongqing Engineering Research Center of Medical Electronics Technology, Chongqing University, Bioengineering College, Chongqing University, Chongqing 400030, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6902-3302","authenticated-orcid":false,"given":"Ning","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biorheological Science and Technology, Chongqing University, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China"},{"name":"Chongqing Engineering Research Center of Medical Electronics Technology, Chongqing University, Bioengineering College, Chongqing University, Chongqing 400030, China"}]},{"given":"Zhong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, China"}]},{"given":"Xi","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biorheological Science and Technology, Chongqing University, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China"}]},{"given":"Zhen-yu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Chongqing Medical University, Chongqing 400016, China"}]},{"given":"Jun","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biorheological Science and Technology, Chongqing University, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China"}]}],"member":"1968","published-online":{"date-parts":[[2017,6,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/0250-6874(83)85036-7","article-title":"Surface plasmon resonance for gas detection and biosensing","volume":"4","author":"Liedberg","year":"1983","journal-title":"Sens. Actuators"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.snb.2010.05.061","article-title":"On the application of gold based SPR sensors for the detection of hazardous gases","volume":"149","author":"Nooke","year":"2010","journal-title":"Sens. Actuators B Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1016\/j.bios.2007.08.025","article-title":"Angle-scanning SPR imaging for detection of biomolecular interactions on microarrays","volume":"23","author":"Beusink","year":"2008","journal-title":"Biosens. Bioelectron."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/j.ab.2006.01.042","article-title":"Surface plasmon resonance imaging analysis of protein-protein interactions using on-chip-expressed capture protein","volume":"351","author":"Kim","year":"2006","journal-title":"Anal. Biochem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"6416","DOI":"10.1093\/nar\/gkl738","article-title":"Fabrication and characterization of rna aptamer microarrays for the study of protein-aptamer interactions with spr imaging","volume":"34","author":"Li","year":"2006","journal-title":"Nucleic Acids Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1246\/cl.2006.678","article-title":"A novel receptor-based surface-plasmon-resonance affinity biosensor for highly sensitive and selective detection of dopamine","volume":"35","author":"Kumbhat","year":"2006","journal-title":"Chem. Lett."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1016\/j.bios.2006.03.012","article-title":"Quantitative and simultaneous detection of four foodborne bacterial pathogens with a multi-channel SPR sensor","volume":"22","author":"Taylor","year":"2006","journal-title":"Biosens. Bioelectron."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.optcom.2012.12.064","article-title":"SPR based immunosensor for detection of legionella pneumophila in water samples","volume":"294","author":"Enrico","year":"2013","journal-title":"Opt. Commun."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1016\/S0956-5663(03)00032-0","article-title":"Immunosensor for detection of legionella pneumophila using surface plasmon resonance","volume":"18","author":"Oh","year":"2003","journal-title":"Biosens. Bioelectron."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.aca.2005.12.069","article-title":"Real-time detection of chlorpyrifos at part per trillion levels in ground, surface and drinking water samples by a portable surface plasmon resonance immunosensor","volume":"561","author":"Mauriz","year":"2006","journal-title":"Anal. Chim. Acta"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1016\/j.bios.2014.09.082","article-title":"Miniature multi-channel SPR instrument for methotrexate monitoring in clinical samples","volume":"64","author":"Zhao","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/S0925-4005(03)00113-8","article-title":"Performance of the spreeta 2000 integrated surface plasmon resonance affinity sensor","volume":"91","author":"Chinowsky","year":"2003","journal-title":"Sens. Actuators B Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/S0925-4005(03)00533-1","article-title":"A portable surface plasmon resonance (SPR) sensor system with temperature regulation","volume":"96","author":"Naimushin","year":"2003","journal-title":"Sens. Actuators B Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2268","DOI":"10.1016\/j.bios.2006.11.026","article-title":"PorTable 24-analyte surface plasmon resonance instruments for rapid, versatile biodetection","volume":"22","author":"Chinowsky","year":"2007","journal-title":"Biosens. Bioelectron."},{"key":"ref_15","first-page":"1759","article-title":"Ellipsometry as a tool to study the adsorption behavior of synthetic and biopolymers at the air\u2013water interface","volume":"17","author":"Benjamins","year":"2010","journal-title":"Biopolymers"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1021\/je60061a006","article-title":"Specific refractive index increments of polymer systems at four wavelengths","volume":"19","author":"Chincholi","year":"1974","journal-title":"J. Chem. Eng. Data"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.sna.2009.12.010","article-title":"A pdms microfluidic system with poly(ethylene glycol)\/su-8 based apertures for planar whole cell-patch-clamp recordings","volume":"166","author":"Xu","year":"2011","journal-title":"Sens. Actuators A Phys."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0022-1759(89)90295-0","article-title":"Immunoassay based on surface plasmon oscillations","volume":"120","author":"Mayo","year":"1989","journal-title":"J. Immunol. Methods"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/978-1-4757-0779-3_3","article-title":"Conformational flexibility in immunoglobulins","volume":"7","author":"Edmundson","year":"1978","journal-title":"Contemp. Top. Mol. Immunol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"29R","DOI":"10.1039\/an996210029r","article-title":"Oriented immobilization of antibodies and its applications in immunoassays and immunosensors","volume":"121","author":"Lu","year":"1996","journal-title":"Analyst"}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/17\/6\/1435\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:39:37Z","timestamp":1760207977000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/17\/6\/1435"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6,19]]},"references-count":20,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2017,6]]}},"alternative-id":["s17061435"],"URL":"https:\/\/doi.org\/10.3390\/s17061435","relation":{},"ISSN":["1424-8220"],"issn-type":[{"type":"electronic","value":"1424-8220"}],"subject":[],"published":{"date-parts":[[2017,6,19]]}}}