{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:13:28Z","timestamp":1760242408959,"version":"build-2065373602"},"reference-count":37,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2017,8,7]],"date-time":"2017-08-07T00:00:00Z","timestamp":1502064000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2017M3A9E2058046"],"award-info":[{"award-number":["NRF-2017M3A9E2058046"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","award":["HI14C3319"],"award-info":[{"award-number":["HI14C3319"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A microsized slit-embedded cantilever sensor (slit cantilever) was fabricated and evaluated as a biosensing platform in a liquid environment. In order to minimize the degradation caused by viscous damping, a 300 \u00d7 100 \u00b5m2 (length \u00d7 width) sized cantilever was released by a 5 \u00b5m gap-surrounding and vibrated by an internal piezoelectric-driven self-actuator. Owing to the structure, when the single side of the slit cantilever was exposed to liquid a significant quality factor (Q = 35) could be achieved. To assess the sensing performance, the slit cantilever was exploited to study the biophysical kinetics related to A\u03b2 peptide. First, the quantification of A\u03b2 peptide with a concentration of 10 pg\/mL to 1 \u03bcg\/mL was performed. The resonant responses exhibited a dynamic range from 100 pg\/mL to 100 ng\/mL (\u221256.5 to \u2212774 \u0394Hz) and a dissociation constant (KD) of binding affinity was calculated as 1.75 nM. Finally, the A\u03b2 self-aggregation associated with AD pathogenesis was monitored by adding monomeric A\u03b2 peptides. As the concentration of added analyte increased from 100 ng\/mL to 10 \u00b5g\/mL, both the frequency shift values (\u2212813 to \u22121804 \u0394Hz) and associate time constant increased. These results showed the excellent sensing performance of the slit cantilever overcoming a major drawback in liquid environments to become a promising diagnostic tool candidate.<\/jats:p>","DOI":"10.3390\/s17081819","type":"journal-article","created":{"date-parts":[[2017,8,9]],"date-time":"2017-08-09T06:32:14Z","timestamp":1502260334000},"page":"1819","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Study of Alzheimer\u2019s Disease-Related Biophysical Kinetics with a Microslit-Embedded Cantilever Sensor in a Liquid Environment"],"prefix":"10.3390","volume":"17","author":[{"given":"Myung-Sic","family":"Chae","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul 02447, Korea"},{"name":"School of Electrical Engineering, Korea University, Seoul 02841, Korea"}]},{"given":"Jinsik","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, College of Life Science and Biotechnology, Dongguk University, Seoul 10326, Korea"}]},{"given":"Yong","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Electrical Engineering, Kwangwoon University, Seoul 01897, Korea"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7344-6060","authenticated-orcid":false,"given":"Jeong","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Electrical Engineering, Kwangwoon University, Seoul 01897, Korea"}]},{"given":"Tae","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Electrical Engineering, Korea University, Seoul 02841, Korea"}]},{"given":"Kyo","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul 02447, Korea"}]}],"member":"1968","published-online":{"date-parts":[[2017,8,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.trac.2016.02.012","article-title":"Recent advances in biosensors for neurodegenerative disease detection","volume":"79","author":"Ganesh","year":"2016","journal-title":"Trends Analyt. 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