{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T03:53:23Z","timestamp":1773978803709,"version":"3.50.1"},"reference-count":57,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2017,8,18]],"date-time":"2017-08-18T00:00:00Z","timestamp":1503014400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Efficient direct electron transfer (DET) between a cellobiose dehydrogenase mutant from Corynascus thermophilus (CtCDH C291Y) and a novel glassy carbon (GC)-modified electrode, obtained by direct electrodeposition of gold nanoparticles (AuNPs) was realized. The electrode was further modified with a mixed self-assembled monolayer of 4-aminothiophenol (4-APh) and 4-mercaptobenzoic acid (4-MBA), by using glutaraldehyde (GA) as cross-linking agent. The CtCDH C291Y\/GA\/4-APh,4-MBA\/AuNPs\/GC platform showed an apparent heterogeneous electron transfer rate constant (ks) of 19.4 \u00b1 0.6 s\u22121, with an enhanced theoretical and real enzyme surface coverage (\u0393theor and \u0393real) of 5287 \u00b1 152 pmol cm\u22122 and 27 \u00b1 2 pmol cm\u22122, respectively. The modified electrode was successively used as glucose biosensor exhibiting a detection limit of 6.2 \u03bcM, an extended linear range from 0.02 to 30 mM, a sensitivity of 3.1 \u00b1 0.1 \u03bcA mM\u22121 cm\u22122 (R2 = 0.995), excellent stability and good selectivity. These performances compared favourably with other glucose biosensors reported in the literature. Finally, the biosensor was tested to quantify the glucose content in human saliva samples with successful results in terms of both recovery and correlation with glucose blood levels, allowing further considerations on the development of non-invasive glucose monitoring devices.<\/jats:p>","DOI":"10.3390\/s17081912","type":"journal-article","created":{"date-parts":[[2017,8,21]],"date-time":"2017-08-21T04:12:17Z","timestamp":1503288737000},"page":"1912","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":85,"title":["A Third Generation Glucose Biosensor Based on Cellobiose Dehydrogenase Immobilized on a Glassy Carbon Electrode Decorated with Electrodeposited Gold Nanoparticles: Characterization and Application in Human Saliva"],"prefix":"10.3390","volume":"17","author":[{"given":"Paolo","family":"Bollella","sequence":"first","affiliation":[{"name":"Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro, Rome 5 00185, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7278-0478","authenticated-orcid":false,"given":"Lo","family":"Gorton","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry\/Biochemistry and Structural Biology, Lund University, P.O. Box 124, Lund SE-221 00, Sweden"}]},{"given":"Roland","family":"Ludwig","sequence":"additional","affiliation":[{"name":"Food Biotechnology Laboratory, Department of Food Science and Technology, BOKU\u2014University of Natural Resources and Life Sciences, Muthgasse 18, Vienna A-1190, Austria"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8990-8506","authenticated-orcid":false,"given":"Riccarda","family":"Antiochia","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro, Rome 5 00185, Italy"}]}],"member":"1968","published-online":{"date-parts":[[2017,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2482","DOI":"10.1021\/cr068069y","article-title":"Electrochemical glucose sensors and their applications in diabetes management","volume":"108","author":"Heller","year":"2008","journal-title":"Chem. 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