{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T07:06:14Z","timestamp":1760425574379,"version":"build-2065373602"},"reference-count":45,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2017,9,13]],"date-time":"2017-09-13T00:00:00Z","timestamp":1505260800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21575076","21621003"],"award-info":[{"award-number":["21575076","21621003"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2016YFA0203101"],"award-info":[{"award-number":["2016YFA0203101"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Beijing Municipality Science and Technology Program","award":["D161100002116001"],"award-info":[{"award-number":["D161100002116001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The enrichment of low-abundance proteins in complex biological samples plays an important role in clinical diagnostics and biomedical research. This work reports a novel one-step method for the synthesis of aptamer-modified graphene oxide (GO\/Apt) nanocomposites, without introducing the use of gold, for the rapid and specific separation and enrichment of human \u03b1-thrombin from buffer solutions with highly concentrated interferences. The obtained GO\/Apt nanocomposites had remarkable aptamer immobilization, up to 44.8 nmol\/mg. Furthermore, GO\/Apt nanocomposites exhibited significant specific enrichment efficiency for human \u03b1-thrombin (&gt;90%), even under the presence of 3000-fold interference proteins, which was better than the performance of other nanomaterials. Finally, the GO\/Apt nanocomposites were applied in the specific capturing of human \u03b1-thrombin in highly concentrated human plasma solutions with negligible nonspecific binding of other proteins, which demonstrated their prospects in rare protein analysis and biosensing applications.<\/jats:p>","DOI":"10.3390\/s17091986","type":"journal-article","created":{"date-parts":[[2017,9,13]],"date-time":"2017-09-13T10:17:32Z","timestamp":1505297852000},"page":"1986","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["One-Step Facile Synthesis of Aptamer-Modified Graphene Oxide for Highly Specific Enrichment of Human A-Thrombin in Plasma"],"prefix":"10.3390","volume":"17","author":[{"given":"Yuan","family":"Xu","sequence":"first","affiliation":[{"name":"Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siyuan","family":"Tan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qionglin","family":"Liang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingyu","family":"Ding","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,9,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1021\/ar400162w","article-title":"Recent advances in molecular recognition based on nanoengineered platforms","volume":"47","author":"Mu","year":"2014","journal-title":"Acc. 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