{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:14:50Z","timestamp":1760242490072,"version":"build-2065373602"},"reference-count":15,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2017,9,2]],"date-time":"2017-09-02T00:00:00Z","timestamp":1504310400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A simple method of nanoparticle decoration can be used in the detection of pneumococcus. After the pneumococcal bacteria were captured by an antibody (pneumococcal C-polysaccharide (PnC) antibody) between the interdigitated electrodes, the gold nanoparticles conjugated with the PnC antibodies were let to bind onto an outer membrane of the bacteria. Upon successfully dense decoration, the bacteria surface will become conductive owing to the metal nanoparticles, and a distinctive conductance change between the electrodes can be observed. Since this success ratio, or the probability of the conductance change, reflects the concentration of the analyte, a number of repeated measurements can be used in the quantification of the bacteria. In this way, we have successfully detected S. pneumoniae in the range of 10\u2013108 CFU\/mL. The limit of detection in this work is lower than that in the commercial detection kit. We hope that the nanoparticle decoration method will play a role in the facile detection of various bacteria.<\/jats:p>","DOI":"10.3390\/s17092012","type":"journal-article","created":{"date-parts":[[2017,9,4]],"date-time":"2017-09-04T11:11:52Z","timestamp":1504523512000},"page":"2012","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Electrical Detection of Pneumococcus through the Nanoparticle Decoration Method"],"prefix":"10.3390","volume":"17","author":[{"given":"Hannah","family":"Pyo","sequence":"first","affiliation":[{"name":"Department of Chemistry, Sungkyunkwan University (SKKU), Suwon 440-746, Korea"}]},{"given":"Cho","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Sungkyunkwan University (SKKU), Suwon 440-746, Korea"}]},{"given":"Daehee","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Sungkyunkwan University (SKKU), Suwon 440-746, Korea"}]},{"given":"Gyuhee","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Sungkyunkwan University (SKKU), Suwon 440-746, Korea"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3886-4579","authenticated-orcid":false,"given":"Sangho","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Sungkyunkwan University (SKKU), Suwon 440-746, Korea"}]},{"given":"Wan","family":"Yun","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Sungkyunkwan University (SKKU), Suwon 440-746, Korea"}]}],"member":"1968","published-online":{"date-parts":[[2017,9,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1128\/CMR.00120-13","article-title":"Biosensors for Whole-Cell Bacterial Detection","volume":"27","author":"Ahmed","year":"2014","journal-title":"Clin. 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Actuators B Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1016\/j.bios.2008.07.071","article-title":"Rapid detection of Bacillus anthracis using monoclonal antibody functionalized QCM sensor","volume":"24","author":"Hao","year":"2009","journal-title":"Biosens. Bioelectron."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.bios.2014.12.040","article-title":"Aptamer-conjugated silver nanoparticles for electrochemical dual-aptamer-based sandwich detection of staphylococcus aureus","volume":"68","author":"Abbaspour","year":"2015","journal-title":"Biosens. Bioelectron."},{"doi-asserted-by":"crossref","unstructured":"Huynh, D.T.N., Kim, A.-Y., and Kim, Y.-R. (2017). Identification of Pathogenic Factors in Klebsiella pneumoniae Using Impedimetric Sensor Equipped with Biomimetic Surfaces. 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