{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T08:25:29Z","timestamp":1783758329027,"version":"3.55.0"},"reference-count":59,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2017,9,15]],"date-time":"2017-09-15T00:00:00Z","timestamp":1505433600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of Korea (NRF) funded by the Korea government","award":["NRF-2016R1A2B4010031"],"award-info":[{"award-number":["NRF-2016R1A2B4010031"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>We developed a versatile and highly sensitive biosensor platform. The platform is based on electrochemical-enzymatic redox cycling induced by selective enzyme immobilization on nano-sized carbon interdigitated electrodes (IDEs) decorated with gold nanoparticles (AuNPs). Without resorting to sophisticated nanofabrication technologies, we used batch wafer-level carbon microelectromechanical systems (C-MEMS) processes to fabricate 3D carbon IDEs reproducibly, simply, and cost effectively. In addition, AuNPs were selectively electrodeposited on specific carbon nanoelectrodes; the high surface-to-volume ratio and fast electron transfer ability of AuNPs enhanced the electrochemical signal across these carbon IDEs. Gold nanoparticle characteristics such as size and morphology were reproducibly controlled by modulating the step-potential and time period in the electrodeposition processes. To detect cholesterol selectively using AuNP\/carbon IDEs, cholesterol oxidase (ChOx) was selectively immobilized via the electrochemical reduction of the diazonium cation. The sensitivity of the AuNP\/carbon IDE-based biosensor was ensured by efficient amplification of the redox mediators, ferricyanide and ferrocyanide, between selectively immobilized enzyme sites and both of the combs of AuNP\/carbon IDEs. The presented AuNP\/carbon IDE-based cholesterol biosensor exhibited a wide sensing range (0.005\u201310 mM) and high sensitivity (~993.91 \u00b5A mM\u22121 cm\u22122; limit of detection (LOD) ~1.28 \u00b5M). In addition, the proposed cholesterol biosensor was found to be highly selective for the cholesterol detection.<\/jats:p>","DOI":"10.3390\/s17092128","type":"journal-article","created":{"date-parts":[[2017,9,15]],"date-time":"2017-09-15T10:22:10Z","timestamp":1505470930000},"page":"2128","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Development of a Sensitive Electrochemical Enzymatic Reaction-Based Cholesterol Biosensor Using Nano-Sized Carbon Interdigitated Electrodes Decorated with Gold Nanoparticles"],"prefix":"10.3390","volume":"17","author":[{"given":"Deepti","family":"Sharma","sequence":"first","affiliation":[{"name":"Department of Mechanical Engineering , Ulsan National Institute of Science and Technology (UNIST), 50 UNIST-gil, Ulsan 44919, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jongmin","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering , Ulsan National Institute of Science and Technology (UNIST), 50 UNIST-gil, Ulsan 44919, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junyoung","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering , Ulsan National Institute of Science and Technology (UNIST), 50 UNIST-gil, Ulsan 44919, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heungjoo","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering , Ulsan National Institute of Science and Technology (UNIST), 50 UNIST-gil, Ulsan 44919, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,9,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.talanta.2015.03.029","article-title":"One-step electrochemical detection of cholesterol in the presence of suitable K3Fe(CN)6\/phosphate buffer mediator by an electrochemical approach","volume":"140","author":"Rahman","year":"2015","journal-title":"Talanta"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.bios.2014.08.029","article-title":"ZnO-CuO composite matrix based reagentless biosensor for detection of total cholesterol","volume":"67","author":"Batra","year":"2015","journal-title":"Biosens. 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